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The microalgal strain Aurantiochytrium sp. shy contains considerable amounts of poly-unsaturated fatty acids (PUFAs), chiefly docosahexaenoic acid (DHA) with potential pharmaceutical and health-related attributes. Effects of various concentrations of glucose, meat extract, monosodium glutamate and sea salt on the algal biomass and DHA production have been investigated in this study. Maximum algal biomass (7.1 g/l) was obtained when the culture medium contained 60 g/l of glucose, 6 g/l of meat extract, 6 g/l monosodium glutamate and sea salt at 25ppt. Lipid contents of the alga exceeded 30% of its dry cell weight, with palmitic acid and DHA as the most abundant components. When the effect of a single additive was concerned, meat extract was significantly effective, while interaction between meat extract and glucose was the most effective in comparison with other interactions (P < 0.0001). According to the results, glucose can assure more algal and fatty acids production when adequate amounts of meat extract exist in the culture medium. Optimal results attained when the ratios of glucose to meat extract and C/N concentrations were 10 and 5, respectively. Due to its remarkable growth rate and the capability to produce substantial quantities of biomass and fatty acids, Aurantiochytrium sp.shy was found to be a major source of the beneficial ingredients, whose productivity can magnify if its culture conditions is optimized using favorable blend of growth-promoting materials.