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Cellulase is one of the industrial enzymes which its production and utilization is increasingly taking into consideration due to global heed to second-generation bioethanol production. Cellulase produced by different organisms such as fungi, bacteria, insects, and plants. With increase in utilization of this enzyme and need for reduction in the enzymes price for production of second-generation bioethanol, the production of recombinant enzyme has been considered noticeably.
In this study, by investigation of corn steep liquor as nitrogen source and second carbon source after glycerol, a new medium is designed based on SYN6 salt medium then biomass and endoglucanase II production by methylotrophic yeast was optimized. Experiments designed by one-factor and response surface methodology used for optimization.
Results showed that optimum conditions for biomass and endoglucanase production are 5.5% (w/v) and 6.15% (w/v) of corn steep liquor respectively. New optimized conditions increased 41.4% and 69.7% for biomass and recombinant enzyme production respectively.