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According to the formation and evolution of life along with static magnetic fields,the permanent exposure has given adaptive ability to beings. Therapeutic magnetism is one of the branches of complementary medicine which uses  the low intensity and non-harmful magnetic fields to the body. By studying in infertile couples (20% male factor), the only cause of infertility and in 50% of cases it is considered as an intermediate factor. One of the influential factors in infertility in men is sperm. In the present study, normal specimens in the magnetic field under the intensities of 1,6 and 12 millitesla and at 1,3 and 5 h intervals.
Sperm movement rate was evaluated by CASA, as well as sperm viability, by eosin staining of necrosin and morphology by staining Papanicula. The results of this step on normal sperm showed a significant reduction in the sperm movement ,which  that was not affected by the field.
Morphological studies also show that sperm motility is not affected by magnetic field.. the survival rate of sperm  was affected by the magnetic field was significantly reduced, and the sperm morphology remained unchanged

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Signal processing has a key role in signal based fault diagnosis in rotating machinery for finding beneficial discriminating features. Task of Signal processing is conversion of the raw data into beneficial features to facilitate the diagnostic operations. the features should be robust regarding noise and working condition of the machine and simultaneously sensitive to the machine defects. Therefore, assignment of more efficient analyzing techniques in order to achieve more beneficial features of the signal and faster and more accurate fault detection is taken into consideration by researchers. In order to finding such features, the current research applies at first wavelet packet denoising and then applies wavelet packet based Hilbert transform as well as improved Hilbert-Huang transform separately to decompose vibration signal into narrow frequency bands in order to extracting instantaneous frequencies. The findings show that the wavelet packet based Hilbert transform generates better results in comparison to the improved Hilbert-Huang transform in detecting frequencies of the broken rotor bar fault.

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Objective: This study attempted to generate monospecific antibodies through immunization with recombinant proteins and subsequent purification by synthetic peptides (the PrIPeP model).

Methods: The SRY gene was cloned on a pet-28a vector and the recombinant protein was expressed in the Escherichia coli (E.coli) BL21 strain. The purified antigen was emulsified in Freund’s adjuvant and injected into rabbits according to a standard time table. Then, a specific peptide was designed, synthesized, and conjugated to sepharose 4B to generate an affinity purification column. As a control, the peptide was conjugated to KLH and used for immunization, as above. Antisera against the conjugated peptide (Pep-antisera) and SRY recombinant protein (Pro-antisera) were evaluated by ELISA and subsequently subjected to the affinity purification column. Sensitivity and specificity of the purified antibodies against SRY recombinant protein as well as negative controls (recombinant HSFY, RBMY, and RPSFY) were assessed by Western blot analysis.

Results: Titration by ELISA confirmed proper immunization and specificity of both antigens. Western blot analysis validated the specificity and sensitivity of the IgG class purified antibodies.

Conclusion: By applying the PrIPeP model, it is possible to develop antibodies against the native structure of a protein whilst avoiding challenges of peptide-carrier protein conjugation.