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Aim and Background: Amylloid fibrils are filamentus protein aggregates derived from various proteins and peptides. They can be distinguished from other type of features according to their appear shape and electron microscope images, also by dye binding methods, which can indicate induced cross beta structures. Amyloid fibrils are correlated to creating general disease, amyloidosis. Disease such Alzheimer, Parkinson, diabetes type II, and others disease which in each of them, the special kind of protein subjected to form amyloid or amyloid like fibrils. A variety of proteins which they are not converted to amyloid fibrils invivo, can be transform to amyloids in special unstabilizing conditions.
Materials and Methods: Congored spectrophotometric method, ThT fluorescence and CD Data was used for fibril formation assay and Transmission Electron microscopy was used for final affirmation of fibrils.
Results: results shows that maximum amyloid formation was in 5 mg.ml-1 protein concentration, 50 ºC and 7.4 buffer pH.

Conclusion:
With the new approach obtained from the kappa casein, amyloid fibers can be introduced as new nanomaterials, Thus the results, given the diverse applications of nanomaterials, can affirm process optimization of amyloid production from accessible and inexpensive protein in milk.

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In recent years, many studies have been performed on for use in various of science. The proper design and synthesis of these has a direct impact on their -chemical properties and their applications, especially in the field of biological sciences. There are several methods for magnetic synthesis. One of the simplest and most efficient methods for synthesis of magnetic is a chemical co-precipitation method, but of magnetic is one of the limitations of this method. In this study, various protocols for the synthesis of magnetic by co-precipitation method and silica coating of magnetic were performed and the effect of different factors such as the type of alkaline compound, the use of , temperature and in dispersion, aggregation of magnetic nanoparticles and their stability in aqueous solutions was investigated. Finally, a simple and reproducible protocol for magnetic synthesis with appropriate size distribution and high dispersion in aqueous solutions was optimized for use in biological applications.

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Government interventions in agricultural markets in order to stabilize commodity prices and producer incomes have had a long tradition. Intervention has been at both the state and country levels and has been for the most part in the form of commodity-based schemes. This study represents an attempt to develop an appropriate methodology for analyzing the aggre-gate effects of a particular type of policy rule. This type of policy rule is one for which the unit of observation is the individual farm unit rather than the individual unit of commodity. The methodology developed in this paper represents an initial attempt to provide the necessary mi-cro-macro modeling with supply response which is required for analyzing the aggregate effects of whole-farm income support programs. The methodology will be illustrated by an empirical application of the aggregate impacts of the whole-farm program in Saskatchewan, Canada.

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Objective: To study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. Materials and Methods: Amyloid fibrils were extracted from CHO cells and binding affinity to Thioflavin T and Congo red was investigated. Then the apple-green birefringence of extracted fibrils was detected under polarized light to confirm the presence of aggregated protein in the extracts. To better investigate amyloid formation in CHO cells, we decided to overexpress an amyloidogenic protein in these cells. To do so, we amplified ProIAPP gene and subcloned it in EGFP-N1 expression vector. Then, CHO cells were transfected with EGFP-N1-ProIAPP and EGFP-N1 as a control. The phenotypes of around 100 transfected cells were characterized for several days after the transfection, using fluorescence microscopy. Additionally, the presence of amyloid structure in these cells was detected by Congo red staining under exposure to polarized light. Cell viability assay was performed using Trypan blue staining. Results: Low level natural amyloid was detected in CHO cells. In addition, the ProIAPP-EGFP transfected cells exhibited aggregated phenotype in which the cells with round morphology had far more aggregates than oval ones. Conclusion: We found amyloid fibrils in CHO cells at low level. Overexpression of amylin protein in CHO cells caused aggregation phenotypes. These cells can be used as a model of cell culture for studying protein aggregation. Amyloidogenic properties of this protein could immensely help us to study the mechanisms that are involved in amyloid formation in mammalians including humans.

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Customer satisfaction represents a modern approach for quality in enterprises and organizations and serves the development of a truly customer-focused management and culture. Measuring customer satisfaction offers an immediate, meaningful and objective feedback about clients' preferences and expectations. In this way, company's performance may be evaluated in relation to a set of satisfaction dimensions that indicate the strong and the weak points of a organization. In this research and for the first time in Iran; Multicriteria satisfaction analysis is offered for measuring and analysis of customer satisfaction in export development bank of Iran (EDBI). The integrated methodology evaluates the satisfaction level of a set of customers based on their values and expressed preferences. This research has been performed in according to request of Customer Affairs and Branch Coordination Department in EDBI that by solution of several linear programming model (with respect to Hierarchical structure of customers' satisfaction dimensions), average satisfaction level and importance (weight) of each dimension will be achieved and finally by development of a set of quantitative indices and perceptual maps makes possible the provision of an effective support for the satisfaction evaluation problem.

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Due to the influence of Internet and mobile service in every part of our lives in addition to pervasive demand for them, next generation wireless networks should be able to address different kind of objectives or demands. New generation of cellular networks must achieve high user quality of experience (QoE) in order to satisfy the user demands and survive in market. To meet this demands, drastic revision need to be made in previous network architecture. This paper reviews some of the key technologies which are emerged to improve future network architecture and meet the demands of users, especially in Fifth generation (5G) cellular network. In this paper, the prime focus is on the air interface of 5G
which includes millimeter wave communication, multiple access technologies, carrier aggregation (CA), and massive Multiple-Input Multiple-Output (MIMO).

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Four hundred strains isolated from Tunisian sheep and goat raw milks were initially screened for their ability to survive the GastroIntestinal Tract (GIT). Forty-three among the four hundred bacteria were resistant to pepsin, pH 2, pancreatin and bile salts at 0.3%, even after 5 hours of incubation. Identification using 16S rRNA gene sequencing was established and we obtained as a species Lactobacillus plantarum (29 isolates from sheep milk and 11 from goat milk) and Lactobacillus pentosus (2 isolates from sheep milk and 1 from goat milk). We showed the ability for auto-aggregation and/or hydrophobicity properties. Finally, both M63 and C78 strains showed an important level of adhesion to three intestinal epithelial cells Caco-2 TC7, HT29-MTX, and HT29-CL.16E. Taken together, these properties allow the lactobacilli strains to be considered promising beneficial strains for developing functional foods for consumers.
 

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In the present research work, the potential probiotic properties of Lactococcus lactis KMCM3 and Lactobacillus helveticus KMCH1 isolated from raw camel milk and traditional fermented camel milk (Chal), respectively, were studied. The probiotic properties of isolates that were investigated included the hemolysis, antibiotic resistance, antibacterial features, resistance to low pH and bile salts, survival under simulated GastroIntestinal Tract (GIT) conditions, adhesion ability to hydrocarbon, and their auto-aggregation and co-aggregation rates. None of isolates exhibited hemolytic activity. They were susceptible against tetracycline, penicillin, ampicillin, chloramphenicol, erythromycin and vancomycin. Lac. lactis KMCM3 and L. helveticus KMCH1 retained their viability at pH 3.0 (8.68 and 8.6 log cfu mL^-1, respectively), 0.3% w/v bile salts (8.23 and 8.58 log cfu mL^-1, respectively) and under simulated GIT conditions (8.31 and 8.46 log cfu mL^-1, respectively). Both of these isolates inhibited the growth of E. coli, S. aureus, L. monocytogenes, B. cereus and S. enterica subsp. enterica serovar Typhimurium with MIC values of 6.25 to 25 mg mL^-1. In addition, They exhibited an ability to adhere to hydrocarbon (xylene), and possessed a high auto-aggregation and co-aggregation rate (more than 40%).
 

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Multi-bar collapsible drum mechanisms are used in various industries such as steel factories, tire factories, etc. In this article, the analysis of a collapsible multi-bar drum mechanism and its optimization is discussed using SAM software. At first, the modeling of the collapsible mechanism is carried out in Catia, and the motion analysis and possible interferences of the mechanism are evaluated. Then the mechanism is entered and analyzed in the SAM software. In this software, the position of the joints along with their movement path is determined, and therefore, it is possible to optimize the position of the mechanism joints in order to avoid interference of the parts.