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Showing 2 results for Bmp-2
B. Onagh, S. Hasannia, F. Heidari,
Volume 10, Issue 3 (9-2019)
Abstract
Bone morphogenetic proteins (BMPs) belong to the superfamily transforming growth factor-beta. These molecules play a role in fetal development and differentiation of different cells. In this regard, two homodimer molecules BMP-2 and BMP-7 play an important role in the formation of ectopic bone So that two types of recombinant form are available for ectopic use. After binding of the homodimer BMP-2 to its receptor at the cell surface, the accumulation of homodimers of type I and II receptors results in a biological response within the cell. Despite the existence of recombinant types of BMP-2 and BMP-7 due to the dangers of their use, the strategy of using monoclonal antibodies to trap endogenous types is still a priority in research programs. Instead of using monoclonal antibodies, the alternative method is to use the natural receptors of the ligand in the body. In this regard, due to the proper Kd binding of the ectodomain component of the receptor II of the BMP molecule in this project, the expression and purification of this part were attempted to trap BMP-2 endogenous. The protein component of the type II receptor ectodomain was expressed and purified by the bacterial host, which, by evaluating CD, of this recombinant protein showed a similar structure to that of the natural type. Also, its binding to the BMP-2 ligand with ELISA was evaluated and then calculated as Kd. Based on the results, the type II receptor ectodomain can be connected to the BMP-2 with a suitable binding property at the nM concentration, and in subsequent studies, it can be used as an alternative to a monoclonal antibody to Trap endogenous BMP molecules.
Zahra Shahsaman, Sadegh Hasannia,
Volume 12, Issue 4 (12-2021)
Abstract
Bone morphogenetic proteins (BMPs) are a subfamily of multifunctional superfamily transforming growth factor-beta (TGF-β), thus they have a lot of similarity in biosynthesis, structure, signaling and biological function with other members of the superfamily. They are involved in growth and differentiation of embryo to maintenance of adult cells. Among this family member, BMP-2 is a valuable protein that acts in different processes such as spinal fusions, articular cartilage damage therapy, tumor inhibition, gingivitis and dental treatment. The high importance of this protein and its low production rate in body caused several researches in the field of producing recombinant BMP-2 in different hosts. Recombinant production of the protein in bacterial host caused the decrease in production costs and therefore led to the common use of BMP-2 in treatment of various diseases. To date, positive effects of intact BMP-2 and its derivative peptides, in order to osteoinduction in fracture treatment and jaw bone regeneration for dental implantation, were considerable. Considering high clinical significance of BMP-2, there is a necessity for more investigations in relation to this protein.
