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Probiotics are live microbial feed supplements, which beneficially affect the host by improving its intestinal microbial balance[8].Recently, researches have shown that lactic Acid Bacteria(LAB), especially Bacillus (because of having endospore) remain stable at cooking temperature and retain their probiotic benefits in baked goods [3]. The purposeof this study,was using of Bacillus coagolans, as a resistant probiotic,for enrichment of  bread. First, these probiotic bacteria were determined by the tests of salt tolerance, heat resistance, bile tolerance, tolerance of acid and pepcin, resistance of antibiotics, and preventing the growth of pathogenic starins[18].Then a certain number cells of  BC were entered in bread dough,before& after  baking the number of live bacteria were calculated by colony count in 1g of dough and bread. The number of BC decreased from 10⁸ to 10⁶ units per gram, after baking. Also,the amount of starch decreasd and changed into simple sugers. The pH was estimated about 4.5-5 and TTA (Total Titritable Acidity) was between 6-8. Finally, the enrichment of bread was  evaluated  by experts and its quality and taste were compared with a control sample. The Results showed this bacterium survives in baked bread and makes good chemical changes on it.

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During the last decade, application of edible coatings as efficient carriers for the transfer of bioactive compounds including probiotic microorganisms has become more prominent for production of food products with stressful condition for these bacteria. In this study, the effect of optimizing a coating formulation based on carboxymethylcellulose and sucrose as carrier of probiotic strain for rock candy coating was evaluated.  Surface response method based on the central composite design was applied to evaluate the coating movement on the rock candy, the amount of coating remaining on the product after immersion and texture characteristics of the coating, such as adhesion. In addition, the textural and rheological properties of the coating solution under different concentrations of carboxymethyl cellulose and sucrose were investigated. The optimized formulation for rock candy coating was obtained with 97.9% carboxymethyl cellulose and 46.5% sucrose. The results showed that by increasing carboxymethyl cellulose concentration (from 0.8 to 1.2%) and sucrose (from 40 to 50%), the viscosity of the coating solution ranged from 9.27 to 82.62 Pa.s. Also, the flow behavior index of the coating solution confirmed the pseudoplastic behavior of the coating at carboxymethyl cellulose concentrations of 0.8 and higher. While, increasing the concentration of sucrose at a constant concentration of carboxymethyl cellulose had a greater effect on the textural parameters of the coating solution. In addition, the use of Bacillus coagulans spores in this product showed high viability of this strain (more than 90%) under product storage conditions (ambient temperature and dry place).

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Today, consumption of milk and its products is one of the signs of development of human societies. Milk is a healthy, nutritious, delicious and thirsty drink that is consumed by a large group of people, especially children. Therefore, the aim of this study was using grape syrup as a flavoring in probiotic milk containing Bacillus coagulants and investigating effects of grape syrup on physicochemical and microbial properties of probiotic milk. For this purpose, two quantitative factors including grape syrup concentrations (0.17, 1, 3, 5 and 5.83 %) and storage time (0.96, 2, 4.5, 7 and 8.04 days) in five levels were evaluated by using a central composite design. The results showed that different concentrations of grape syrup and storage time on pH, acidity, dry matter, fat and viability of B. coagulation of probiotic flavored milk were significant (P<0.05). The optimum condition was obtained based on maximum pH and viability of B. coagulation and minimum acidity. The optimum condition was 1% grape syrup and 2 days’ storage time, which its total desirability was 95%.

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Today, yogurt is produced as the most important probiotic dairy product worldwide and marketed. The combination of prebiotics with probiotics is called synbiotics, which have more beneficial effects on host health. This study was performed to compare the effect of inulin and green banana flour on the properties of synbiotic yogurt during 28 days of refrigerated storage. Inulin and green banana flour were used at 2% levels in synbiotic yogurt containing Lactobacillus plantarum and Bacillus coagulans and compared with control (probiotic) yogurt. The results showed that with increasing storage time, the acidity of all treatments increased and the pH value decreased. Addition of inulin and green banana flour increased the acidity and decreased the pH in the synbiotic samples, but no significant difference was observed between the two prebiotics used. Dry matter content in synbiotic treatments was significantly higher than control treatments (P <0.05).The presence of both prebiotic compound had a positive effect on the survival capability of probiotic bacteria in yogurt. The effect of inulin on the survival of Lactobacillus plantarum was greater than of green banana flour, but in treatment of Bacillus coagulans there was no significant difference between the two prebiotics. Count of Lactobacillus plantarum and Bacillus coagulans was higher than the recommended limit of 6 Log cfu /g in all samples of synbiotic yogurt during storage for 4 weeks. Survival of Bacillus coagulans were higher than Lactobacillus plantarum. According to the results of this study, it can be said that green banana flour has pre-biotic properties and is effective in maintaining the survival of probiotic bacteria, so it can be concluded that this combination has a high potential for use in healthy foods.

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Today, consumers' interest in consuming healthy foods with high nutritional value has drawn the attention of everyone, especially researchers, to the use of healthy foods, further the use of probiotics and prebiotics in bakery products, especially sourdough bread. Bulk bread samples prepared with sourdough containing probiotic Bacillus coagulans and prebiotic fructooligosaccharide (FOS) were characterized for their physical, chemical, and sensory attributes. The results showed that by adding sourdough containing B.coagulans to bulk bread compared to the control, acidity, specific volume, moisture, height, crust hardness, cohesiveness, springiness, chewiness, and sensory evaluation decreased but hardness increased. In this study, water activity was not influenced by factors. In contrast, adding sourdough containing B.coagulans and FOS to bulk bread significantly affected hardness, chewiness, crust penetration, color, and hardness during storage, as well as sensory evaluation. However, it significantly reduced the specific volume, height, and moisture indices compared to the control sample. Consequently, sourdough containing B.coagulans and FOS has provided bread with desirable properties and may be used as a starter culture for creating bulk bread with high nutritional and functional properties.