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In this study the effect of three dietary treatments including; two concentrations (10⁶ and 10⁷ cells per ml) of the freshwater alga Chlorella vulgaris and one treatment of baker's yeast, Saccharomyces cerevisiae) on growth, protein and total and free amino acid composition of freshwater rotifer Brachionus calyciflorus were studied. Results showed that rotifers fed with high concentration of algae (10⁷cell/ml) had significantly higher growth rate (0.56 ± 0.03) than other treatments. Maximum density of rotifers fed high concentration (1790 ± 10.7 ind/ml) significantly was higher than low concentration (525±41.1 ind./ml) and baker's yeast +oil treatments (115±2.1 ind/ml). The crud protein of rotifers fed yeast was significantly higher than rotifers fed with low density of algae (p<0.05). Total amino acids of rotifers fed yeast, low concentration and high concentration of algae were obtained 250.66±16, 112.15±10 and 198.82±14 mg/ g of sample, respectively that there were significant difference between them(p<0.05). The highest and lowest amounts of free amino acids were observed in rotifers fed yeast (8.77±1.1) and low concentration of algae (4.04±0.3), respectively (p<0.05). Based on this study it can be concluded that rotifers fed with algae showed a higher growth rate compared with those fed the yeast, although protein and free amino acids in rotifers fed yeast were higher in compared algae treatments. In addition, treatment with high concentrations of algae, amino acids showed a relative increase in the body. 

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Phosphate ions is one of the chemical pollutants that enters to the surface and underground water through municipal, industrial and agricultural wastewater. The aim of this study is to investigate the removal of phosphate from wastewater by Chlorella vulgaris and Spirulina platensis. To this purpose the microalgae added to 350 ml wastewater in lab experiment. In order to evaluate the effect of phosphorus removal, the experiment were performed during 8 days. The initial dry weight of Chlorella vulgaris and Spirulina platensis in wastewater samples were 0.02 and 0.05 g/L, respectively. During the growth period, the concentration of phosphate in aqueous solution were measured on days 1st, 4th, 6th and 8th by APHA standard methods through Bio spec-1601 spectrophotometer. Based on the results, the percentage of phosphorus removal by Chlorella vulgaris was 40.65, 38.0124.45 and 72.21 , and by Spirulina platensis was 20.13, 20.01, 10.44 and 42.79 for days 1 to 4, 4 to 6, 6 to 8 and 1 to 8, respectively. The final dry weight of Chlorella vulgaris and Spirulina platensis in wastewater samples were 3.93 and 1.93 g/L, respectively. From this study it can be concluded that the algae are able to remove phosphorus from wastewater and Chlorella vulgaris has higher ability of reducing phosphorus from waste water, and could be good candidate to reduce the phosphate in wastewater treatment.