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The white cheese has high percentage of fat, (saturated and cholesterol) which is not desirable from human health point of view. The aim of this study was to produce low cholesterol white cheese (Feta) by the substitution of milk fat with sunflower oil (S.O) and determine its effects on the chemical, microbial and sensory parameters of the white cheese. For this S.O (1.5, 2.5 and 3.5 percent) and monoglyceried stearat as emulsifier (5percent) were added to the milk containing (0.05, 1 and 2 percent fat). The chemical, microbial and sensory tests were done on the samples. The fatty acids profile and the cholesterol were also determined (GC). The results showed that by addition of the S.O, cholesterol and saturated fatty acids (SFAs) were reduced and the percent of unsaturated fatty acids (USFAs) increased significantly (p<0.05). In cheese samples with 1.5, 2.5 and 3.5 percents of S.O, the amount of cholesterol reduced from 115.1 to 66.2, 29.1 and 18.8 milligrams respectively and the percents of the reduction of SFAs were 43.2, 30.2 and 16.7 milligrams. The amounts of the USFAs increased significantly (p<0.05) in compare with the control. In sensory evaluation tests the samples with 1.5 and 2.5 percents of S.O preferred in acceptance by panels. The samples with 3.5 percent S.O had significant differences (P<0.05) in sensory properties with the other samples. The ratio of 1:2.5 (milk fat: S.O) was the proper substitution ratio which on that the amount of cholesterol and SFAs were reduced about 59 and 75 percents in the samples with the 29.1 and 30.2 milligram cholesterol and SFAs to compare with the control and the percent of USFAs increased from 27.3 to 68.3.

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Probiotic products highly affect the health of consumers by reducing the risk of heart attacks and improving the desirable microflora in the intestinal tract. In this study the effect of yogurt (500g/d for 3 wk) enriched with either Bifidobacterium bifidum or Lactobacillus acidophilus on the level of cholesterol, Low Density Lipoprotein (LDL) and High Density Lipoprotein (HDL) of serum and as well on the intestinal microflora were investigated in 24 healthy volunteers. Statistical analysis indicated that lower levels of cholesterol and HDL were found after consumption of either one of bio-yogurts as compared to the control states (P<0.05), whereas changes of LDL were not significant. Also, in comparison with control periods the results of microbial counts indicated that the number of fecal coliforms excreted during the consumption of either one of the yogurt types were not noticeable, while the number of Bifidobacterium bifidum and Lactobacillus acidophilus increased significantly (P<0.05).

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Mayonnaise is a kind of oil in water emulsion, that most widely used sauce in the world. Egg yolk is a key ingredient in mayonnaise, due to excellent emulsifying properties. Nevertheless, one main problem with egg yolk is its high cholesterol content, so different attempts have been carried out to develop low cholesterol mayonnaise. The aim of this study was to prepare low cholesterol mayonnaise containing wheat germ protein isolate (WGPI) and xanthan gum (XG) as egg yolk substitutes. The mayonnaise prepared with different composition of WGPI, egg yolk (0-9% of each component) and XG (0-0.5%), as formula variables by applying the optimal mixture design approach. Effect of this substitution on rheological characteristics and microstructure of mayonnaise samples were evaluated. The results indicated that all mayonnaise samples exhibited thixotropic shear thining behavior under steady shear tests, which fitted by Power law model and the factors related to this model were specified in the samples. Also, it was observed that mayonnaises exhibited weak- gel like properties, by using dynamic oscillatory shear tests and had significant effects on acquired parameters from frequency sweep test, such as storage modulus, loss modulus and tan delta. According to rheological and microstructure results, the low cholesterol mayonnaise contained WGPI and XG shows similar characteristics to those of a typical mayonnaise. Formulation 10 (71.5% and 4.2% egg replacement with WGPI and XG, respectively) shows the most similarity to control sample.  

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  Production of imitation dairy products is one of the novel accomplishments in dairy industries.  Imitation dairy products range from complete dairy substitutes, with no dairy ingredients, such as soy milk, to products that have a high percentage of dairy ingredients, contain some non-dairy constituent.These products are appeal to manufacturers and consumers in terms of economical and nutritional features, however, it must be noticed the imitation dairy products must be resemble in terms of apparent, sensory and textural attributes as much as possible to real dairy product. Cheese is the product which is produced in high amount but also other dairy products such as butter, types of cream, yoghurt, milk, milk powder, infant formulae and concentrated milks are produced as imitation dairy products. Imitation dairy products alike of every novel and synthetic products have encountered with pro and con. In this article, in addition of introduction of this products, we decided deal to agreeable and against aspects about of them.

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 In this experiment, a total of 128 Hy-line W₃₆ hens at 63 weeks of age were used. The hens were put at random into 4 treatment groups (4 replicates and 32 hens per treatment) and were fed four different diets: three diets with different levels of Spirulina (1.5, 2.0 and 2.5%) and one control group based on wheat and soybean meal. All birds were housed in commercial cages, had ad libitum access to water, and were fed 100 g bird^-1 per day. Egg production, feed intake, feed conversion ratio, egg weight, yolk index, Haugh unit, shell thickness, shell weight, specific gravity, egg yolk cholesterol, and yolk color were compared with the control group. Egg yolk color was measured by the BASF Ovo-color fan. Our results indicated that these traits did not show any significant changes with the Spirulina addition (P> 0.05), while a significant increase in egg yolk color was observed in the treatments that received the Spirulina (P< 0.0001). Yolk color scores of the control group and different levels of Spirulina (1.5, 2.0 and 2.5%) were 1.5, 10.5, 11.4 and 11.6 in BASF color fan, respectively. There were not significant differences between the treatments with 2.0 and 2.5% of Spirulina. In conclusion, this study can suggest use of 2.0~2.5% of Spirulina in diet to produce an aesthetically pleasing yolk color.

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In this study, the effects of Culban seed (Vicia peregrine L.) on live body weight, feed intake, feed efficiency, egg production, egg quality characteristics, egg yolk fatty acid profiles and yolk cholesterol content in laying hens were investigated. The experiment consists of seven treatment groups with ten replications containing one laying hen in each and for 32 weeks old seventy laying hen were used in the study. The hens were fed the experimental diets containing 10 and 20% Culban seed meal in raw and autoclaved form. In addition, the autoclaved form was supplemented with enzyme (Bacozymex). There was also a control group which was fed a Culban meal free-diet. The lighting regime was 16.5 hours light and 7.5 hours darkness. The experiment lasted sixty three days. The supplementation with Culban seed had significant effects on feed intake, feed conversion ratio, egg production, albumen index, egg weight, eggshell weight, shell strength, haugh unit, yolk lipid, yolk cholesterol ratio and yolk total poly unsaturated fatty acids. The presence of culban seed in feed has negative effects on the overall performance of laying hens, mortality, internal and external quality of eggs. Therefore, in alternative protein sources Culban seed. Therefore Culban seed could be recommended as a protein source (up to 20%) for laying hen diets.

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Objective: Familial hypercholesterolemia (FH) is an autosomal dominant disease mainly attributed to mutations in the low-density lipoprotein receptor gene (LDLR). This study aims to investigate molecular changes in the LDLR gene in patients with high cholesterol in individuals from Ardebil Province, Iran.
Methods: We evaluated 100 patients with suspected FH from Ardebil Province. DNA samples using primers LDLR gene and exon 10 PCR-SSCP method was tested and modified bands on gel electrophoresis detected and subsequently examined by DNA sequencing.
Results: We evaluated 100 patients with suspected FH, 43 males and 66 females. The average age of 50.68 and 281.81 had average cholesterol levels of the subjects. In this study, we identified a polymorphism 1413G(A LDLR gene. Allele G, 70% of the population studied and the A allele is 30% of the subjects to be included.
Conclusion: The findings of this study showed that polymorphism of the LDLR gene in FH 1413GA was not the main role, but could indirectly affect, and possibly other exons of the gene or other genes in the development of FH in the region have.

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Phytosterols are among the most important plant sterol compounds. This study was conducted to identify and determine the amount of phytosterols in distributed yogurts in Tehran by gas chromatography method without derivatization. A total of 60 yogurt samples, including 20 pasteurized high-fat yogurt samples, 20 pasteurized cream yogurt samples, and 20 traditional high-fat yogurt samples, were collected randomly from dairy product supply centers in Tehran from January to March 2022. Then, the amount of cholesterol and four phytosterol compounds including brassicasterol, campesterol, stigmasterol, and beta-sitosterol in yogurt samples were determined using gas chromatography assay without derivatization. The results showed that the total sterols in high-fat, creamy, and traditional yogurt samples were 504.08±55.79, 816.21±51.87, and 332.8±41.22  mg/100mL, respectively. The highest and lowest percentage of cholesterol belongs to cream (%54.75±065) and traditional yogurt (%46.57±0.65), respectively(p<0.05). Also, the highest percentage of phytosterol belongs to traditional yogurt(%54.75±065) and there was a significant difference with other yogurts(p<0.05). The results also showed that the total phytosterols were more than the permissible limit of the national standard (3%) in all samples. According to the results, phytosterols are used in various yogurts distributed in Tehran, which is against the national standard of Iran. Therefore, periodic and regular evaluation of phytosterols in distributed yogurts should be done throughout the year by regulatory bodies and prevent the violation of consumers' rights.
 

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In this study, molecular identification of Lactiplantibacillus pentosus v390 was performed using 16S rRNA gene analysis with FYM27 and R1492 primers. The viability of the strain was assessed under acidic conditions at pH 5.2, 5.3, and 5.4, and resistance to bile at concentrations of 0%, 3.0%, 5.0%, and 7.0% was investigated. Antioxidant activity, cholesterol absorption, hydrophobicity, potential for DNase enzyme production, biogenic amines, hemolytic activity, and resistance to common therapeutic antibiotics were evaluated. The antimicrobial effect of the strain against pathogenic bacteria (Shigella dysenteriae, Salmonella enterica serovar Typhimurium, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Bacillus subtilis) was examined using the well and disk diffusion method. The results showed that the L. pentosus v390 strain had the ability to survive at different pH levels, but after three hours of storage at pH 5.2, the bacterial count decreased. The strain demonstrated growth capability at various bile salt concentrations. L. pentosus v390 was intermediate to the antibiotic Nitrofurazone, resistant to Nalidixic and Imipenem antibiotics, and sensitive to Vancocin, Gentamicin, Chloramphenicol, Penicillin, and Ciprofloxacin antibiotics. The hydrophobicity, antioxidant activity (DPPH and ABTS), and cholesterol absorption of the strain were 38.0 ± 50.46%, 40.0 ± 20.37%, 45.0 ± 9.39%, and 47.0 ± 50.36%, respectively. No DNase enzyme production, biogenic amines, or hemolytic activity were observed from the strain. L. pentosus v390 exhibited stronger antimicrobial effects against Gram-positive bacteria. The results indicated that L. pentosus v390 has desirable probiotic properties that require further research to confirm its application potential in food product development.
 

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Introduction: Statins are one of the approved drugs used in the clinic, which are prescribed to reduce the amount of cholesterol in the blood of patients. However, the effects of the drug in reducing the amount of fat and the occurrence of side effects are not the same in the patients. Considering the role of LncRNAs in regulating gene expression, the possible role of HOTAIR LncRNA and atorvastatin treatment in regulating HMGCR gene expression as the main regulating gene in cholesterol synthesis has been investigated.
Methods: Bioinformatics analyses were used to find common regulatory factors between the HMGCR gene and candidate LncRNAs. MTT assay was used to determine the optimal dose of atorvastatin treatment on the HepG2 cell line. RNA extraction, cDNA synthesis, and quantitative analysis of gene expression were performed by qPCR. Finally, HMGCR protein expression was evaluated via the Western blot technique.
Results: Bioinformatic analyses showed that there is a relationship between HMGCR expression and some LncRNAs (HOTAIR, TUG1, MALAT1, GAS5, JPX, DLX6AS). In the cell culture, atorvastatin treatment increased the expression of HMGCR at mRNA and protein levels in the HepG2 cell line. Among the candidate lncRNAs, HOTAIR LncRNA expression decreased by 80% under atorvastatin treatment. Downregulating of the HOTAIR gene led to increased HMGCR gene expression at the RNA and protein levels.
Conclusion: The results of this study indicated that, aside from blocking the HMGCR enzyme binding site, atorvastatin can regulate the expression of HMGCR mRNA and protein by changing the HOTAIR expression.