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Laccase (benzenediol: oxygen oxidoreductase), is a multicopper polyphenol oxidase enzyme which has glycoprotein structure. The Researches are indicated that laccase enzyme can play role in detoxification of aromatic pollutants (as petroleum derivatives) and conversion them to less toxic compounds. On the other hand, because of its extensive, fibrous root system; Festuca arundinacea, creates an appropriate environment that causes increased catalysis of petroleum contaminants. Considering the fact that increasing in catalysis of pollutants can be provided by presence and changes in activity of various plant enzymes, in this research; the changes in laccase activity of Festuca's vegetative organs under soil pollution with different concentration of diesel fuel has been investigated. For this purpose, at first, the seeds of Festuca were cultivated in pots containing diesel fuel polluted soils and also control pots in greenhouse conditions. Then in specified time treatments, plants were harvested and plant extract containing laccase were extracted from aerial parts and roots of the plant, separately. After doing centrifuge, changes in enzyme activity were calculated by spectrophotometer. The results show that creation of soil pollution treatments compared with control samples, leads to increase in laccase activity in many cases. In other words, by increasing in laccase activity, the plant will increase its potency of decomposition and assimilation of pollutant hydrocarbons.

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In this research, biodiesel was initially produced from waste vegetable oil by transesterification reaction. The main properties of this fuel were compared with the ASTM D-6751 standard.then, performance of MF-399 tractor engine was tested and evaluated by using 5 to 25 percent biodiesel and diesel blends. Test results showed that, the power and torque of MF-399 tractor engine were increased, using biodiesel and diesel blends. This is because of good combustion of biodiesel due to high oxygen content of this fuel. There was also a slight increase in the fuel consumption and specific fuel consumption of biodiesel and diesel blends due to low calorific value of biodiesel. Results show that the B5D95 blend has the best performance and the lowest increase in specific fuel consumption among the other blends. The fuel consumption and specific fuel consumption of B25D75 was lower than the B20D80 blend. Therefore, if the goal is using high amount of biodiesel, B25D75 blend is recommended for use in MF-399 tractor engine.

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The aim of this study is numerical investigation of a evaporating and non-reacting diesel spray operating in a high pressure and high temperature constant volume combustion chamber, as an essential step in simulation of liquid fuels combustion. To this end, the impact of droplets diameter distribution on estimating two critical characteristic parameter i.e. liquid and vapor penetration lengths is studied using the open-source OpenFOAM code. In order to determine droplets diameter distribution effect, three different distribution ranging from 0.25-100 micron is chosen and the liquid and vapor penetration lengths are individually calculated for each distribution. The results are validated against the experimental data published by Sandia National Laboratory. The results show while the droplets diameter distribution has a remarkable effect on the predicted value of the liquid length, so that leads to overestimate liquid penetration lengths up to more than two times; its effect on the vapor length prediction is negligible. Also assuming a nozzle diameter distribution leads to non-physically increase in the value of liquid length. This non-physically prediction may lead to misleading prediction of spray impingement to piston and the cylinder walls resulting an error in unburnt hydrocarbons concentration as well as the engine efficiency estimation.