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Showing 3 results for Disk Diffusion
Volume 4, Issue 3 (9-2015)
Abstract
Some plant extracts have been reported to exhibit antimicrobial properties. Plant based pesticides appear to be an alternative for the synthetic pesticides because of their less dangerous impacts. The objective of this study was to assess the inhibitory activity of Brassica napus water extract on mycelial growth of six phytopathogenic fungi Rhizoctonia solani, Phythophtora drechseleri, Pythium aphanidermatum, Verticellium dahliae, Fusarium oxysporum, and Gaeumannomyces graminis. Antifungal activity test was performed by disc diffusion method. Concentrations of 100 and 50 ppm had the highest and the lowest inhibitory effects on all studied species respectively. The shoot extract (SE) was significantly exerted higher antifungal activity than root extract (RE). At 100 ppm, V. dahliae (17.02% inhibition by RE) and F. oxysporum (50% inhibition by SE) were the most sensitive species however, R. solani (1.8 and 15%) was the most resistant fungus to both extracts. Active compounds of B. napus extract were determined by high performance liquid chromatography (HPLC). Phenolic compounds had the highest concentrations in extracts and are probably the main cause of the mycelial growth inhibition. Application of canola aqueous extract or incorporation of canola in crop rotation program can be considered as a method for management of some soil-borne phytopathogenic fungi.
H. Abbasi , E. Kamrani, N. Amrollahi Biuki, M. Ehsanpoor ,
Volume 9, Issue 3 (9-2018)
Abstract
Aims: No study has been conducted on the antibacterial activity of sea anemone in the Persian Gulf region; thus, the aim of the present study was to investigate the antibacterial, antifungal, and cytotoxic effect of Stichodactyla haddoni from Persian Gulf.
Materials and Methods: In this experimental study, sea anemone samples were collected from the coast of Persian Gulf (Hormoz Island) and their extraction was performed with organic solvents such as methanol, dichloromethane, and acetone. The effect of the extract obtained on human pathogenic bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, and fungus strains such as Candida albicans, Aspergillus niger was tested, using agar disk diffusion method. The cytotoxicity of this anemic was evaluated, using brine shrimp test (BST) method on Artemia salina. The data were analyzed by SPSS 19, using Probit regression test.
Findings: The extracts studied in this study showed little anti-bacterial effect. In antifungal assay, inhibition zone was showing maximum of 17mm at 16µg concentration against Aspergillus niger of acetone extract of S.haddoni. The methanolic extracts of S.haddoni tissue had a higher cytotoxicity with less LC50 (609 330/μg/ml) than acetone and diclormethanic extracts.
Conclusion: Methanolic, acetone, and diclormethanic extracts, from S.haddoni have an antifungal effect on their antibacterial properties. It also has significant cytotoxic effects, which is higher in methanolic extract than acetone and diclormethanic extracts.
Volume 18, Issue 112 (6-2021)
Abstract
Resistance of microbial strains to antibiotics and controlling food safety are two of greatest global problems. In this research phenolic content, flavonoids, antioxidant capacity and antibacterial properties of Sclerorhachis platyrachis essential oil were evaluated. Phenolic content and flavonoids of Sclerorhachis platyrachis essential oil were measured using Folin ciocalteu method and aluminum chloride colorimetric method respectively. Antioxidant capacity evaluated using DPPH, ABTS and β-carotene bleaching assay. Agar well diffusion, agar disk diffusion, minimum inhibitory concentration and minimum bactericidal concentration were used to evaluate antibacterial activity of essential oil against some pathogens. Results showed that total phenolic content and flavonoid of Sclerorhachis platyrachis were 55.63 mg GAE/g and 62.28 mg QE/g respectively. Antioxidant capacity using DPPH, ABTS and β-carotene bleaching assay were 52.39, 68.3 and 49.95 percent respectively. In disk diffusion method the highest and lowest zones were 16.2 and 9.7 mm, belonged to Staphylococcus aureus and Enterobacter aerogenes respectively. In all strains MBC was higher than MIC and Gram-negative bacteria were more resistant to essential oil. Sclerorhachis platyrachis essential oil showed favorable antibacterial and antioxidant capacity and its usage will inhibit growth of pathogenic bacteria.
