Aims: In irreversible electroporation process, the membrane of cancer cells is damaged irreversibly by electric pulses of high-intensity field, which in turn leads to cell death. Factors influencing the field distribution include voltage, pulse width, and electric conductivity of tissue. The present study was conducted with the aim of evaluating conductivity changes of liver tissue during irreversible electroporation and calculation of the electric field distribution.
Materials and Methods: In the present experimental study, using simulation, the relationship between pulse width and voltage intensity of each pulse was investigated in conductivity changes during irreversible electroporation, and the electric field distribution was calculated. In this simulation, in order to solve the equations, the software COMSOL 5 was used. Needle electrodes were used, and the liver tissue was considered as the target tissue. Eight pulses with the stimulated frequency of 1Hz, pulse width of 100µs and 2ms, and the intensity of the electric fields ranging from 1000 to 3000v/cm were used as electric pulses.
Findings: Conductivity of tissue increased during sending the electrical pulses. The conductivity changes in the tip of the electrodes were more than the area between the two rows of electrodes. As the intensity of the pulsed electric field increased, the tissue conductivity also increased. When the conductivity of the tissue was constant and variable, the maximum electric field intensity was obtained 3879 and 3448v/cm.
Conclusion: While electric pulse transmission, tissue conductivity increases. The electric field distribution depends on the conductivity at the desired point and by changing this conductivity due to the electroporation, the electric field distribution also changes and the maximum intensity of the electric field decreases.

Introduction: Sometimes materials that do not have the ability to penetrate into the membrane will need to be widely entered into the cell. Therapeutic methods are among the methods that sometimes feel this change in permeability when using different drugs and genes. Electroporation (EP) is a new technique that increases the permeability of cell membranes when cells exposed to an external electric field more than a threshold voltage and is used to introduce different non-permanent molecules. The major application of EP in the treatment of cancer combined with chemotherapy drugs such as Bleomycin and Cisplatin is electrochemotherapy. The aim of this study was to review the electroporation of cells, using electric and magnetic fields with approach of cancer treatment.
Conclusion: In pre-clinical studies, this method has first been optimized on the animal and cell, and after clinical trials, today, the standard and clinical protocol of electrochemotherapy has been proposed as a safe and effective method for some tumors. This is a simple method with minimal side effects, but in new pre-clinical studies, with the use of high frequency electrons, low electromagnetic fields, and the use of pulsed magnetic fields, it has been tried to overcome the limitations of this standard method.