Showing 3 results for Heparin
Shahla Hemmati, , , ,
Volume 5, Issue 1 (11-2014)
Abstract
Abstract Sharks are relatively large sea creatures by an extensive cartilaginous skeleton. The shark cartilage is a rich source of bioactive molecules including collagen protein and glycosaminoglycan. In the present study, Cetyl Piridinium Chloride cationic salt was used for extracting of glycosaminoglycans from dryed cartilage of Carcharhinus dussumieri and their anticoagulant properties were examined. FTIR spectrum was also used to identifing and structurally compare with heparin. The total amount of the extracted glycosaminoglycan was 42.8 mg/g of the dry cartilage. Also, FTIR spectrum results confirmed the presence of heparin- like compounds in the extract. Finally, the anticoagulant properties of extracted glycosaminoglycans was examined by the Activated Partial Thromboplastin Time anticoagulant test (APTT) method in 410, 763, and 1250 concentrations, and Prothrombin Time (PT) method in the 1250 concentration on the human plasma. The anticoagulant time was 43, 50, and 85s in 410, 763, and 1250 concentrations of extracts, respectively, which extended the coagulation time 1.3, 1.5, and 2.5 folds.
M. Bahri , S. Hasannia, B. Dabirmanesh , H.h. Zadeh,
Volume 10, Issue 4 (12-2019)
Abstract
Introduction: Nowadays, bone tissue repair with increasing bone disorders and injuries have special importance. Bone tissue engineering provided specific solutions to these problems. The present study was conducted with the aim of purification of recombinant fusion peptide containing hydroxyapatite affinity tag using the ceramic chromatography column.
Material & methods: In this study, a fusion peptide was designed which at one side comprised the heparin-binding domain sequence, which can be attached to various types of growth factors involved in tissue repair and entrap these factors at the site of the lesion. On the other side, it contained a tag, which included a sequence derived from a laboratory study based on phage expression. The reason for keeping the sequence of this tag is to attach the peptide to the scaffold containing hydroxyapatite and purifying the recombinant peptide by the hydroxyapatite column. Therefore, the gene sequence was optimized and synthesized for expression in the prokaryotic host of E.coli strain BL21. Then the gene sequence was subcloned by double digestion with the SacI and BamHI enzymes into the expression vector of pET-21a(+). The expression of the recombinant peptide was investigated by SDS-PAGE and western blot. In order to optimize the purification conditions, two-step purification was carried out by applying fundamental changes in the main work method of the manufacturer company and was purified with acceptable purity. Finally, the existence of peptide assemblies was investigated by the SLD method.
Finding: The results of PCR cloning, enzymatic digestion using SacI and BamHI enzymes and sequencing indicated the accuracy of the cloning process. On the other hand, expression of the fusion peptide was confirmed by SDS-PAGE and Western blot techniques, and its migration onto the gel resulted in a band cleavage of about 12 kDa. Changes made to the manufacturer's workflow allowed the purification process to be optimized and the results of the DLS method showed the purity of the purified peptide.
Conclusion: The results indicate the desirable expression and remarkable purity of the fusion peptide designed in this study.
Volume 17, Issue 3 (5-2017)
Abstract
In-stent restenosis is one of the important inefficient reasons about Drug Eluting Stents (DESs). Awareness of how polymer coated drug distributes by these devices provides valuable informations about its efficacy. Porous media theory has been employed in the modeling of drug polymer and the injured arterial wall composed of media and adventitia. The stabished coupled PDEs describing local pharmacokinets of heparin has been solved numerically by finite volume method. Two approaches, single phase and two phases models, has been chosen for coating and the effect of local mass non-equilibrium dynamics in the coating on drug distribution has been evaluated by allocating three magnitude for solid-liquid transfer time characteristic. Moreover, the effect of lost drug by vasavasorum and microcapilaries has been considered as well as cell metabolism. The results show a significant change in drug concentration distribution in the presence of phase change happening. Reducing in solid-liquid transfer time characteristic is associated with drastic reducing in both drug egression from polymer and wash out from adventitia and has a pleasant effect. Also, consumtion of drug declines concentration level in the wall dramatically, specially in adventitia.
