Search published articles
Showing 3 results for Hepatitis B Surface Antigen
Volume 9, Issue 2 (8-2023)
Abstract
Backgrounds: Several studies have elucidated vitamin D as an important immunomodulatory factor regulating immune responses to different viral infections and vaccines. This study aimed to evaluate the impact of 25(OH) D serum levels on immune responses to hepatitis B virus (HBV) vaccine.
Materials & Methods: This study was conducted on 134 healthy individuals aged 18-35 years, referring to health centers for HBV vaccination in Mane and Samalghan city in North Khorasan, Iran from June to September 2021. Demographic data were collected through a questionnaire. Serum 25(OH) D levels were analyzed using commercial sandwich ELISA kits. Anti-hepatitis B surface antibody (anti-HBsAb) levels were determined in blood samples 4-6 weeks post-vaccination.
Findings: The prevalence of vitamin D deficiency and insufficiency among the participants was 46.3 was 34.3%, respectively. The level of 25(OH) D was insignificantly higher in women than in men. There was no significant association between serum 25(OH) D levels and participants' ethnicities and BMI ranges. Anti-HBsAb titer was significantly higher in participants with sufficient vitamin D levels compared to those with insufficient and deficient levels (1835 ± 252.55 vs. 1129 ± 120.7 and 1363 ± 0.125 ng/ml). Serum anti-HBsAb levels post HBV vaccination were significantly higher in women and younger individuals than in men and older individuals, respectively.
Conclusion: This study findings suggest that participants with different serum vitamin D levels produce seroprotective antibody titers post HBV vaccination, while those with sufficient vitamin D levels may produce higher titers against HBV vaccine.
H. Rashedi , S. Arjmand , H. Rashedi, S.o. Ranaei Siadat, M. Pouryaqubi,
Volume 9, Issue 3 (9-2018)
Abstract
Aims: Hepatitis B is a viral infection, which can cause serious liver problems. Hepatitis B surface antigen (HBsAg), which is produced as recombinant, is used to produce the Hepatitis B vaccine. The aim of this study was to detect DNA aptamer with high affinity against HBsAg by Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
Materials and Methods: In the present experimental study, SELEX method was used to isolate and sequence a DNA aptamer with high affinity against HBsAg. The affinity of this monoclonal nucleotide sequence was calculated by fluorimetric method. The difference of initial absorption and residual value as a measure for the number of associated sequences were calculated with Prism 5 software by nonlinear regression method, Binding-saturation and one site-total model were performed, and the amount of electron affinity (Kd) was determined.
Findings: After performing the SELEX procedure and evaluating the amplified sequence with agarose gel, the result was positive control sample containing a bond in the range of 72nucleotides, indicating successful amplification of the selected sequence, using selective primers. During cloning steps from existing colonies of PCR reaction with aptamer specific primers, the presence of aptamer was confirmed in Escherichia coli bacteria. The reported aptamer had a stable secondary structure with a free energy of ΔG of less than -6.9kJ and Tm higher than 45°C.
Conclusion: The selected DNA aptamer has a high affinity to the target protein (HbsAg) and can be considered as an alternative for mAbs in chromatography column.
Volume 10, Issue 4 (12-2024)
Abstract
Background: Viral infections may play a significant role in the development of heart failure, especially in people with related cardiac conditions such as myocarditis. Hepatitis B and D viruses (HBV-HDV) are potentially fatal liver infections. This study examined the influence of metabolic syndrome and its associated disorders.
Materials & Methods: This cross-sectional study investigated hepatitis D antibody and hepatitis B surface antigen (HBsAg) in 239 people aged 35 to 65 years in Mashhad, Khorasan Razavi Province, Iran in 2018-2019. There were two study groups: those with cardiovascular diseases (CVD) and healthy individuals. Serum samples of all subjects in both groups were examined using enzyme-linked immunosorbent assay (ELISA).
Findings: HDV infection was detected in none of the study groups. Only one patient (0.8%) in the case group tested positive for HBsAg. The average LDL (low-density lipoprotein) (p= .8) and cholesterol (p= .3) levels in terms of lipid profiles were similar in both groups. Although the mean high-density lipoprotein (HDL) level in the patient group was lower, the mean triglyceride level in this group was higher than in the control group. Fasting blood sugar (FBS) (p= .009) and aspartate aminotransferase (AST) levels were significantly higher in CVD patients, while 59.3% of them exhibited metabolic syndrome.
Conclusion: This study results demonstrate no connection between HBV/HDV infection and cardiovascular diseases. The findings confirm that metabolic syndrome and hyperglycemia are underlying factors in cardiovascular diseases. Changes in HDL and triglyceride levels could impact cardiovascular diseases more than other lipids.
