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Genus Medicago belonged to family Fabaceae is one of the most important forage legumes. Annual species of this genus are indigenous to Mediterranean region and used for prevention of soil erosion, green manure and forage. In this research, genetic variability and classification of 14 annual medicago truncatula genotypes using ISSR markers was done. 9 out of 15 ISSR primers which possessed suitable polymorphism and amplification were used for fingerprinting of studied genotypes. Totally, 71 bands were amplified via ISSR primers which 11 bands were monomorphic and 60 bands were polymorphic across genotypes. Based on Jaccard similarity coefficient, minimum similarity (0.25) was seen between TN8.3 (Tunisia) and TN6.18 (Tunisia) and maximum similarity (0.82) was seen between TN8.3 (Tunisia) and SA28064 (Cyprus). Population structure analysis using STRUCTURE software subdivided them into 9 subpopulation. In this study, maximum admixture was occurred in TN1.21 (Tunisia), A10 (Australia), F83005-5 (France), SA22322 (Syria), A20 (Australia), and DZA315-16 (Algeria) genotypes. Results revealed that annual self-pollinated M. truncatula had noticeable genetic variation which Is accurately detectable using ISSR molecular markers

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Rye is one of Iran's most important crops, known as Secale, belonging to the Poaceae. In this research, genetic diversity of 39 families of rye populations from different regions of Iran, the USA, and the Soviet :union: was evaluated with the ISSR marker. The results showed that 8 ISSR initiators produced 48 bands which included 18 polymorphic bands (37.5% polymorphism). The mean of polymorphic information content (PIC) and marker index value (MI) for ISSR primers was 0.15 and 7.2 respectively. The highest PIC (3.0) was related to primer 5+6 and the highest MI (0.96) reached for primer 1+6. After observing polymerase chain reaction products on an agarose gel and scoring DNA bands, the analysis was performed with NTSYS software. The cluster analysis dendrogram of UPGMA and Jaccard's similarity coefficient divided the rye populations into 9 groups, the results were compiled with grouping by principal component analysis. The results of analysis of Molecular Variance indicated an in-species variation more than inter-species variation. The mean Nei genetic variation (h) was 350 and the mean of Shannon index (I) in rye species was 523, which indicates a relatively good variety within species. The results showed that the ISSR marker was a useful tool in determining genetic variation of inter and intra specific of rye.