Showing 4 results for In Vitro Culture
F. Rezanejad, A.s. Hosseini,
Volume 10, Issue 3 (9-2019)
Abstract
Physalis alkekengi L. is planted in gardens and green spaces because of the beautiful and colorful sepals surrounding the fruit. The species is widely used in traditional medicine and treating a range of diseases. Micropropagation of P. alkekengi was evaluated using the node and internode explants. After sterilization and seed germination, sterile seedlings were transformed to basal MS medium to create sterilized seedlings as a source of explants. Regeneration of nodes and internodes explants was studied at various concentrations of growth regulators of 2, 4-D and BAP as well as in medium lacking growth regulators or control (11 various media). The experiment was conducted in a completely randomized design with three replicates. The internodal explants produced shoot on media 2 (0.2mgl-12, 4-D), 3 (0.2mgl-12, 4-D+0/2mgl-1 BAP ), and 4 (0.5mgl-12, 4-D+0.2mgl-1 BAP ) and then were rooted on these media. The nodal explants in control and different hormonal treatments generated shoots; interestingly, shoots generated in control medium successfully established roots on the same medium after 7 days (70%). The other regenerated shoots in different media (10) were rooted on ½ MS medium containing 1mgl-1IBA. The rooted plants were transplanted into pots containing sand as well as perlite to be well acclimated before transfer to the greenhouse. They grew well later in the greenhouse at a 100% success This study shows high in vitro regeneration capability of this species as an important medicinal and ornamental plant. Therefore, it is suggested to use this species in molecular and genetic studies, somaclonal variation, and the production of herbal medicine.
Fereshteh Heidargholinezhad, Yousef Hamidoghli, Valiollah Ghasemiomran, Porya Biparva,
Volume 15, Issue 4 (10-2024)
Abstract
The production of secondary metabolites of medicinal plant
Volume 16, Issue 6 (11-2014)
Abstract
Taxus chinensis var. mairei is a rare and endangered medicinal plant species distributed in China. In order to promote fast propagation and preserve the natural resources, conditions for in vitro germination and seedling development of embryos of T. chinensis var. mairei from Anhui or Zhejiang were investigated. Results showed that in vitro germination rate of excised embryos cultured under 14 hours photoperiod was higher than that in darkness. But, nearly all embryos germinated under 14 hours photoperiod failed to develop into seedlings. Comparatively, 23.3 and 36.3% of embryos from Anhui and Zhejiang, respectively, which germinated in darkness, developed into full seedlings. Addition of plant growth regulators [gibberellic acid(GA3), indole-3-acetic acid (IAA), 6-benzylaminopurine (BA)] and organic additives (casein hydrolysate and yeast extract) in mediums promoted germination and seedling development. (Woody plant medium) WPM medium supplemented with 0.5 mg L-1 GA3, 0.5 mg L-1 IAA, 0.5 mg L-1 BA and 1 g L-1 activated charcoal was optimal for the culture of embryos from Anhui, while WPM medium supplemented with 0.5 mg L-1 GA3, 500 mg L-1 casein hydrolysate and 1 g L-1 activated charcoal was optimum for embryos from Zhejiang. Moreover, the germination and seedling survival rate of embryos of T. chinensis var. mairei decreased with increasing maturity of the seeds. In conclusion, darkness during germination is necessary for subsequent seedling development and immature seeds are optimal for embryo culture of this species.
Volume 22, Issue 4 (10-2019)
Abstract
Aims: The aim of the present study was to evaluate the effects of lysophosphatidic acid (LPA) supplementation of human ovarian tissue culture media on the morphology of tissue and alteration in angiogenesis by expression of vascular endothelial growth factor (VEGF) after transplantation.
Materials & Methods: In the present experimental study, the human ovarian tissues (n=8) after collection from female-to-male transsexual people, were cut into small fragments (n=98). Then, vitrified-warmed and cultured 24 hours in two groups in the presence and absence of LPA, and finally they were transplanted to γ-irradiated mice (n=13). After two weeks the morphology of tissues was studied by hematoxylin and eosin staining and VEGF protein was detected by immunohistochemistry. The expression of VEGF gene was evaluated by real time RT-PCR.
Results: The morphology of both transplanted tissues was well preserved and follicles at different developmental stages were seen in all studied groups. Significantly a higher expression of VEGF gene was observed in the LPA-treated group compared to the non-treated once (p<0.05). Several blood vessels were shown positive reactions for VEGF antibody as green color in stroma of ovarian tissue sections in all studied groups.
Conclusion: Supplementation of human ovarian tissue culture media with LPA before transplantation could increase the expression of VEGF gene related to angiogenesis.
