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, , , , ,
Volume 6, Issue 1 (10-2015)
Abstract
In this study,we investigated the biosurfactant -producing microorganisms . Samples of the oil wells , , stable and Ilam were collected and transferred to the Scientific and Industrial Research Organisation biotechnology collaboration of 45 strains isolated from the IROST and the best combinations of isolates selected strains produced using different culture conditions were the order of 3M mineral salts medium containing glucose , molasses and Lindhard Taguchi method was used in these experiments, various factors were analyzed . The best conditions are obtained to produce rhamnolipid with medium Lindhard in vitro rate of 8/8 g rhamnolipid and ability emulsification of crude oil, 88 percent of the dry weight of cell 2/2 g , therefore, the intended environment for the production the fermenter was used in the state parameters shaking, C/N ratio and aeration efficiency and maximum production of rhamnolipid 2/14 grams per liter of capacity emulsification of crude oil 2/98 and the dry cell 4/3 g of was 23 % and the highest oil recovery of crude oil saturation in the core when the system is equal to 42% was achieved.
H. Karbalaei-Heidari, L. Taghavi, P. Hasani Zadeh,
Volume 11, Issue 1 (3-2020)
Abstract
Biosurfactants are valuable microbial metabolites that have considerable applications in different industries. They offer so many advantages over their synthetic counterparts such as biodegradability, low toxicity, activity at extreme conditions, ability to be produced from renewable wastes and by-products. In the present study, biosurfactant production of Halomonas sp. MM93 in nutrient broth medium at 30°C after 72h was investigated using oil spreading and hemolysis tests. The emulsification capacity of the biosurfactant was also evaluated in a defined production medium during 96h. Effect of olive oil, n-Hexan, and kerosene as hydrophobic carbon sources to induction of biosurfactant production by the strain MM93 was also investigated. Due to the importance of stability in the case of industrial use, the effect of extreme temperature, pH and salinity on the stability of bacterial culture supernatant was evaluated. This strain created a clear zone of 2.5cm diameter in an oil-spreading test and its E24 index was 45%. Halomonas sp. MM93 could reduce the surface tension of the culture medium from 70 to 40 mN/m. Also, the produced biosurfactant showed remarkable stability at high temperature (100°C), extreme acidic and alkaline conditions (pH=2-12), and high salinity (20g/L). According to obtained data, native isolated moderately halophilic bacterium, Halomonas sp. MM93 could be considered as a potent strain in terms of producing stable biosurfactants for various industries especially the processes of increasing microbial recovery of oil that need Compounds with High surface activity and high stability.
