Showing 5 results for Nanocurcumin
S.s. Mortazavi Farsani, M. Sadeghizadeh, H. Shirzad, F. Najafi,
Volume 10, Issue 4 (12-2019)
Abstract
Aims: Hematopoietic stem cells are responsible for the production of blood cells in the bone marrow. During the process of differentiation, these cells commitment to two precursor cell lines include myeloid and lymphoid cells. Various blood cells, excluded lymphocytes, generates from myeloid cells. Some patients with severe anemia or thrombocytopenia receive hematopoietic stem cell through transplantation. Finding a potential component for inducing differentiation of hematopoietic stem cells before transplantation, could be an appropriate strategy for the acceleration of blood cells production in recipient persons. Various studies indicate the ability of Curcumin for inducing of cell differentiation. This component can alter many of cellular mechanisms.
Material and methods: The aim of this project was to evaluate the effects of Nanocurcumin on mRNA expression levels of GATA1, GATA2, c-Myb and Hhex genes and alteration of cellular ROS in umbilical cord blood-derived hematopoietic stem cells. Nanocurcumin was synthesized from Curcumin, Oleic acid, and PEG400. The rate of Nanocurcumin delivery into the cells was also evaluated.
Findings: Our results show that intracellular ROS and expression levels of GATA1, c-Myb, and Hhex transcription factors were significantly increased after treatment with Nanocurcumin (p<0.05). These transcription factors involve in myeloid differentiation.
Conclusion: Enhancement of these transcription factors expression making Nanocurcumin a potential candidate for applying in myeloid differentiation media and basic and clinical studies.
S. Karimian Bahnamiri, A. Maghsoudi, F. Yazdian,
Volume 10, Issue 4 (12-2019)
Abstract
Aims: Curcumin is a natural molecule that due to its various curative effects including antibacterial properties, it can be used as a medicine, albeit after reducing its disadvantages. The aim of the present study is to develop a method for preparation of nanoparticles of curcumin using PAA, PVA, and PEI polymers with a view to improve its stability, increasing bioavailability and aqueous solubility as well as study its effectiveness against methicillin-resistant to Staphylococcus aureus.
Materials & Methods: In order to synthesize polymeric nanoparticles including curcumin with the nano-precipitation method, optimizing the effective concentration of polymer, curcumin, and water were determined by using the Response Surface Method (RSM). Synthesized nanoparticles were characterized by Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS) and zeta potential measurement methods. Furthermore, minimal concentration inhibitory of synthesized nanoparticles against the Staphylococcus aureus resistant to methicillin was measured.
Findings: The created nanoparticles were round, discrete and smooth in surface morphology and the average particle size for PAA, PVA, and PEI were 149±7nm, 175±8nm, and 184±9nm respectively. The minimum inhibitory concentration for PAA, PVA and PEI nanoparticles against the Staphylococcus aureus were 0.480±0.024, 0.390±0.019 and 0.340±0.017mg/ml. The concentration of solvent, polymer, and curcumin was important to obtain small size particles.
Conclusion: The results indicated that the water solubility of curcumin significantly improved by particle size reduction up to the nano range. The inhibitory property of curcumin nanoparticles has greatly increased due to the smaller particle size and their increased penetration into the bacteria and nanoparticles loaded with curcumin could be a promising drug carrier for the treatment of cancer, infections and other diseases.
Volume 14, Issue 2 (6-2011)
Abstract
Objective: Curcumin, is the active component in turmeric (Curcuma long). This agent induces apoptosis via inhibiting various signaling pathways. However, its poor aqueous solubility prevents its widespread application. In this study, dendrosomes with water-solubility, nano-sized dimensions and nontoxic properties, was used for curcumin delivery to cells.
Materials & Methods: In the present study, the potential of dendrosomes for use as a drug delivery system was assessed in AGS, HT3, 5637, hBMSC and U87 cell lines. In order to achieve optimal concentration of drug and the most suitable cell line, the effects of different concentrations of free and dendrosomal curcumin was examined on the cell lines. Propidium iodide staining was used for determining apoptosis induction and the expression of Bax gene was investigated by semi-Q RT-PCR.
Results: Dendrosomal formulation significantly improved water solubility of highly hydrophobic curcumin in AGS cells. Flow cytometry analysis indicated 48 percent of cells treated with dendrosomal curcumin and 20 percent of cells treated with free curcumin (at the optimal concentration of drug) underwent apoptosis after 18h. Semi-Q RT-PCR results exhibited the increase of expression of Bax pro-apoptotic gene in cells treated with dendrosomal curcumin.
Conclusion: Dendrosomal formulation, compared to free curcumin, enhanced curcumin solubility and increased apoptosis induction in treated cells. These data, together with the observation of a 50 % increase of Bax gene expression confirmed the apoptotic effects of dendrosomal formulation of curcumin.
Volume 19, Issue 3 (11-2016)
Abstract
Objective: The human genome consists of protective genes, which contain a sequence known as the antioxidant responsive element (ARE) located in their promoter regions. ARE is specific to the transcription factor NF-E2 related factor2 (Nrf2). This signaling pathway is the major defense mechanism against oxidative stress that comprises the chemoprotective response. The cell line that expresses the ARE reporter is sensitive for the detection of ARE activating compounds. It can help to identify toxicity risk and antioxidant activity of chemicals and drugs.
Methods: We used a stable Huh7 ARE-reporter cell line in this study. Metabolic activity of these cells in different concentrations of lead (0 to 80 micromole) was evaluated by the MTT test. We assessed the effects of oxidative stress. We exposed the Huh7 ARE-reporter cell line to different concentrations of lead, an oxidative stress inducer, and nanocurcumin, an antioxidant compound, after which we investivated luciferase activity. Real-time PCR was used to detected AREKEAP1 pathway gene expression.
Result: Lead, at 30 μM, suppressed 50% of the cells’ metabolic activity.Cells treated with both lead (30 μM) and nanocurcumin at 4 μM and 8 μM had decreased luciferase activity compared to those treated with only lead. This activity increased when we increased the nanocurcumin concentration to 16 μM. Real-time PCR analysis showed decreased NQO1 and NRF2 expressions in cells treated with both lead (30 μM) and nanocurcumin (4 μM and 8 μM) compared to just lead treated cells. However, NQO1 and NRF2 had increased expressions in cells treated with both lead (30 μM) and nanocurcumin (16 μM) compared to just lead treated cells.
Conclusion: Nano curcumin, as an antioxidant, significantly reduced the toxic effects of lead toxic. This effect probably occurred through the AREKEAP1 pathway. Hence, nanocurcumin could be used as an antioxidant to reduce oxidative stress.
Volume 19, Issue 4 (9-2017)
Abstract
Objective: There are numerous strategies to prevent hepatotoxicity caused by doxorubicin therapy. These strategies include exercise as well as herbal antioxidants such as curcumin to reduce the toxic effects of doxorubicin. This study aims to evaluate the effects of six weeks of continuous training with and without nanocurcumin supplementation on doxorubicin-induced hepatotoxicity in an aging rat model.
Methods: We randomly divided 42 Wistar male rats into 7 groups: control saline, control doxorubicin, nanocurcumin + doxorubicin, nanocurcumin + saline, continuous training + doxorubicin, continuous training + saline, and continuous training + nanocurcumin + doxorubicin. The rats received intraperitoneal injections of D-galactose (100 mg/kg) to induce ageing. The training groups ran on a treadmill for six weeks, five days per week with a gradual increase from 25 min/day to 54 min/day at a velocity of 15 m/min to 20 m/min. In the last fifteenth days, rats scheduled to received doxorubicin had a cumulative dose of 15 mg/kg of body weight (daily: 1 ml/kg). Nanocurcumin supplement (daily: 100 mg/kg body weight) was administered to the respective groups. Assessment and analysis were conducted after homogenization of the liver tissue biopsy.
Results: Doxorubicin caused a significant decrease in glutathione peroxidase and a slight increase in malondialdehyde in the liver. On the other hand, continuous training with doxorubicin treatment prevented the decrease of glutathione peroxidase and increase in malondialdehyde in the liver that was caused by doxorubicin. Also, six weeks of continuous training with nanocurcumin supplementation caused a significant decrease in malondialdehyde and increased glutathione peroxidase in the liver compared to the control doxorubicin group.
Conclusion: Based on the results, the combination of nanocurcumin supplementation and continuous training in the doxorubicin-induced aging rat model have led to a precautionary effect and up-regulation of antioxidant defense. Continuous training appeared to have more beneficial effects than nanocurcumin supplementation in reducing doxorubicin-induced hepatotoxicity.
