Showing 12 results for Salicylic Acid
Volume 3, Issue 4 (12-2014)
Abstract
The effect of salicylic acid (SA) on induction of resistance against root-knot nematode (Meloidogyne javanica) and the effect of M. javanica to induce biochemical defense responses in tomato (Solanum lycopersicum L.) roots at six-leaf stage were investigated. Meanwhile, the effect of different concentrations of SA on mortality of second stage juveniles of M. javanica was examined. Changes in the activity of cytoplasmic peroxidase (POX), catalase (CAT) and phenylalanine ammonia lyase (PAL) enzymes in the roots of tomato seedlings were measured during seven successive days after inoculation with M. javanica in greenhouse. SA was used as soil drench and leaf spray. The efficiency of treatments were evaluated by measuring diameter of galls, number of galls per plant, number of egg masses per plant, number of eggs per individual egg mass, root and foliage fresh weights. The results showed that use of SA as soil drench and leaf spray significantly reduce diameter of galls 28% and 32%, number of galls per plant 40% and 44%, number of egg masses per plant 45% and 49% and number of eggs per individual egg mass 53% and 55% compared to control (inoculated with nematode only). The activity of the enzymes (POX, CAT and PAL) increased in comparison with plants treated with distilled water. The maximum level of larva mortality was observed at 7 mM SA with no significant difference at concentration of 6 mM. SA caused 21.2% mortality of larvae at concentration of 5 mM.
Volume 6, Issue 1 (3-2017)
Abstract
One of the effective plant disease management strategies is based on the employment of resistance inducers. In the present study, to assay, the effects of Salicylic acid (chemical inducer) and Piriformospora indica (biological inducer) on wheat powdery mildew (Blumeria graminnis f. sp. tritici), the expression rate of Non-expresser of pathogenesis-related genes1 (NPR1) genewas evaluated using qPCR. For this purpose, Falat and Tajan cultivars were selected as susceptible and resistant genotypes to powdery mildew, respectively. To evaluate the rate of gene expression, the P. indica colonized Falat along with mock plants were inoculated with Powdery mildew. In another experiment, Falat treated with SA and control plants were inoculated with Powdery mildew 48 h after treatment with SA. Gene expression was assayed in Falat compared with resistant cv. Tajan. Sampling was carried out at 0, 6, 12, 24 and 48 hours after infection. Comparisons of gene expression patterns showed that after infection, the expression levels of NPR1 increased in induced and non-induced Falat and Tajan cultivars. The maximum gene expression levels were observed at 24 hours post infection. But the expression levels of the gene at this timewere much higher in induced treatments compared with control. The current study showed that NPR1 can be involved in resistance strategy. Thus, using NPR1 gene as a desired gene in genetic engineering for increasing the potential of plant resistance to pathogens can be considered. Moreover, the high response of NPR1 gene in induced plants indicated that both SA and P. indica play a critical role in inducing resistance.
S. Mahmoodi Tarkhorani , F. Sanjarian Dehaghani, M. Monsef Shokri ,
Volume 10, Issue 1 (3-2019)
Abstract
Aims: Thymus Garden (Thymus vulgaris L.) is one of the economically important plants which is extremely sensitive to oxidative stress and drought stress during germination time. Salicylic acid, as an herbal hormone, plays an important role in increasing plant tolerance to biotic and abiotic stresses. The current study was conducted aiming to increase the plant resistance to environmental stress by increasing its enzymatic and non-enzymatic antioxidant capacity by salicylic acid treatment.
Materials & Methods: In this experimental study, the plant seeds were soaked in 2mM salicylic acid solution a randomized complete block design with three replicates for 16 hours, and they were then planted in pots. Pots were transferred to growth chamber with constant and controlled conditions for 16 hours of light: 8 hours of dark at a temperature of 25°C for 14 days. At the end of the experiment, the growth parameters of plants, germination percentage, phenol content, and the activity of the important antioxidant enzymes, such as superoxide dismutase, catalase, polyphenol oxidase and peroxidase, were measured and compared with the control group.
Findings: Although salicylic acid did not have a significant impact on plant growth, it has led to an effective of antioxidant enzymes in the plant. Moreover, this treatment has increased the antioxidant content of the plant.
Conclusion: Treatment with salicylic acid could result in an increase in Garden Thyme tolerance to stress conditions.
Volume 10, Issue 1 (10-2021)
Abstract
Septoria tritici blotch (STB) caused by Zymoseptoria tritici is one of the most important wheat diseases in the world and causes significant annual damage to wheat crops around the globe. The use of resistant cultivars is the most effective method for the management of this disease. Recently, the use of acquired systemic induced resistance has been proposed to manage wheat leaf blotch. In this study, the effect of salicylic acid (SA) on the relative changes of PAL and PR2 gene expression was investigated using qPCR technique. The expressions of catalase, peroxidase, and ascorbate peroxidase enzymes were also assessed in a sensitive wheat cultivar. Controlled and contaminated plants were sampled and compared at 0, 12, 24, 48, 96, and 240 hours after inoculation. The results showed that treatment with salicylic acid significantly reduced the level of disease compared to control plants. Comparison of gene expression patterns also showed that the expression of both PAL and PR2 genes in control and SA pre-treated plants increased after fungal inoculation; however, their expression was significantly higher (2.6 and 1.3 folds respectively for PAL and PR2) in plants treated with salicylic acid than the control. Moreover, treatment with salicylic acid significantly affected the activity of all three enzymes. It can be concluded that the high response of PAL and PR2 genes to salicylic acid pre-treatment, as well as increased activity of peroxidase along with the reduced activity of catalase and ascorbate peroxidase enzymes indicate the effective role of SA in inducing wheat resistance against STB.
Volume 13, Issue 3 (12-2024)
Abstract
Root and foliar application of exogenous salicylic acid (SA), a critical plant defense hormone, has enhanced adult host resistance to Fusarium head blight (FHB), one of the most devastating fungal wheat diseases. In contrast, information on the direct effects of FHB pathogens on in vitro growth has been hardly available. To elucidate this, the antifungal activity of different concentrations of SA was investigated on four Fusarium pathogens with diverse pathogenicity under in vitro conditions. SA inhibited mycelial growth and conidial germination of all pathogens in potato dextrose agar medium in a concentration-dependent manner, with the greatest inhibition achieved using the highest SA concentrations. The antifungal activity of SA on mycelial growth was found to be pH-dependent and more efficient in acidic conditions than in alkaline conditions. However, the inhibitory effect of SA on conidia germination is not due to acidification of the medium. Microscopic observations reveal that mycelium from inhibition zones showed a morphological alteration compared to normal mycelium from the untreated control. Our results demonstrate for the first time that, although the inhibitory effect of SA required acidic growth conditions to be observed, SA has a significant and direct impact on F. culmorum, F. solani, F. verticillioides and F. equiseti through a reduction in efficiency of germination and growth at higher concentrations. This study has shown that SA could be considered a potent and promising antifungal to FHB infection that is safe for the environment.
Volume 13, Issue 6 (11-2011)
Abstract
The present research was carried out under hydroponic culture to evaluate the interaction between salicylic acid (SA), nickel (Ni) on vegetative and reproductive growth of strawberry plants as a factorial experiment in a completely randomized design. Well-rooted daughter plants of Pajaro cultivar, were potted in 3 L plastic pots and were sprayed with SA at concentrations of 0, 1, 2 and 3 mM and NiSO4 at 0, 150 and 300 mg.L-1, after establishment. Results indicated that SA at 2 mM increased root and shoot fresh weight, number of inflorescences and fruits, fruit nitrogen concentration and yield. The effect of Ni was promoting too, as 150 mg.L-1 of Ni led to significant increments of fruit number, inflorescences, leaf area per plant and yield. However, the best results were found when SA was applied accompanied by Ni.
Volume 16, Issue 86 (4-2019)
Abstract
The aim of this study was to investigate the effect of Aloe vera gel (50 and 100% w / w), salicylic acid (0 and 2 mM) and thyme extract (0, 0.1 and 0.5% v / v) on shelf life of fresh pistachios. Disk diffusion test was done in a completely randomized design and the percentage of Aspergillus development, microbial count, sensory evaluation and color tests were assessed in a factorial based on a completely randomized design. The results showed that the mold growth development (in disk diffusion test) and total counts of mold, yeast and aerobic mesophilic bacteria were lower in the samples containing Aloe vera gel, salicylic acid and thyme extract. Also, the highest score and color parameters in color and sensory evaluation tests were found for the samples with above mentioned coating, which had a significant difference with the control (P <0.05). Control and treatment with Aloe vera gel of 50% concentration (a) were identified as the worst coatings, and Aloe vera gel coating containing thyme extract and salicylic acid at high concentrations (AST) were found to be the best treatment with the ability to improve the shelf life of fresh pistachios.
Volume 16, Issue 88 (6-2019)
Abstract
The Mazafati date is one of the most important export products of Kerman province, which their quality characteristics may change during storage. The use of edible coatings is a new technique for increasing the shelf life of various agricultural products. The aim of this study was to investigate the effect of Aloe vera containing salicylic acid (2 mM) and green tea extract (0.5%) on physicochemical, microbial and sensory properties of stored dates at 4 and 25 °C for 150 days. The results of analysis of variance showed that the coating, temperature and time of storage had significant effects on the evaluated parameters (p <0.05). According to the results, the lowest weight loss was observed in samples coated with Aloe vera containing green tea extract and salicylic acid. This type of coating prevented significant changes in pH, acidity, total soluble solids (°brix), total and reducing sugar contents of date samples compared to the control during the storage period. During the storage period, the pH and total sugar content decreased, and acidity, total soluble solids and reducing sugar content increased. Total phenolic content did not have a clear trend during the storage period and its amount in the coated samples was significantly higher than the control. Aloe vera containing green tea extract and salicylic acid also effectively prevented microbial growth. In addition, panelists gave higher scores to dates coated with Aloe vera containing green tea extract and salicylic acid. The changes in physicochemical, microbial and sensory parameters in the stored dates at 25 °C were significantly higher than that stored at the temperature of 4 °C. In general, the use of Aloe vera containing green tea extract and salicylic acid, and storage at 4 °C is recommended due to the reduction of microbial growth and maintaining the quality characteristics of Mazafati date.
Volume 16, Issue 92 (9-2019)
Abstract
Apricot is a climacteric fruit with high respiration rate, which ripen quickly during the postharvest stage and has a short shelf life. Therefore, the use of environmentally friendly compounds for delaying the ripening process is one of the safe methods for extending the shelf life and reducing the postharvest losses of this fruit. Hence, the effect of some chemical treatments on quality and shelf life of apricot fruit cultivar ‘Shahroudi’ was carried out in a completely randomized design with seven treatments and four replications. The treatments were fruit immersion in individual or combination solutions of salicylic acid, oxalic acid and nitric oxide at concentrations of 2, 2 and 1 mM, respectively. The fruits were then packed and transferred to 2 °C with relative humidity of about 85 ± 5%. After 4 weeks of storage, their chemical, sensorial and qualitative properties were evaluated. The results showed that the highest fruit firmness was obtained in salicylic acid (11.46N) and nitric oxide treatment (9.85N). Soluble solids content had the highest values in salicylic acid and combined treatments, respectively. In terms of organoleptic evaluation, salicylic acid and nitric oxide treatments significantly preserved the texture, taste and appearance of the fruits. The highest shelf life was observed in salicylic acid (27.5 days) and nitric oxide (25.5 days) treated fruits, while it was only 14 days in control. In general, it can be concluded that salicylic acid and nitric oxide were the best treatments.
Volume 18, Issue 5 (9-2016)
Abstract
The main objective of this study was to assess the effectiveness of individual application and combination effect of salicylic acid treatments (0, 0.5, 1 mM) in both unpacked and Modified Atmosphere Packaging (MAP) on changing biochemical compounds and extending postharvest life of nectarine. Fruits were stored at 0°C with 90% RH for 40 days. Experimental fruits were analyzed for weight loss, soluble solids, titratable acidity, fruit firmness, total phenolic compounds, flavonoids, antioxidant capacity and overall quality during the storage period at 10 day intervals. The results showed that total phenolic, flavonoid concentrations and antioxidant activity fluctuated progressively until the 30 days of storage, and then decreased until the end of the experiment in all applied treatments. Salicylic acid treatment especially at 1 mM salicylic acid concentration with MAP had a positive effect on biochemical compounds. Furthermore, the combined treatment of MAP had a clear advantage over the other treatments in reducing weight loss, retarding softening, increasing shelf life, and maintaining higher overall fruit quality. The results indicated that salicylic acid with MAP can be used as a safe alternative chemical to keep the quality and for storage of nectarine.
Volume 24, Issue 1 (1-2022)
Abstract
Systemic Acquired Resistance (SAR) as a management strategy for plant parasitic nematode is a state of resistance increased after a previous infection of plant to a biotic pathogen. Induction of SAR is accompanied by local and systemic enhancement of Salicylic Acid (SA). SA increase in plant is concomitant with PR1 expression. We examined the effect of three chemicals including SA, Abscisic Acid (ABA), and DL-β-Amino-n-Butyric Acid (BABA) on the root-knot nematode Meloidogyne incognita on tomato plants. The expression of PR1 genes and 9-Cis-Epoxycarotenoid Dioxygenase (NCED) as markers for SAR and ABA-related activity genes was investigated in growth chamber conditions. Results showed that all elicitors reduced the population of nematode as compared to the control. Shoot length, shoot fresh and dry weight of nematode infected tomatoes pre-treated with BABA increased by 20, 25, and 8 % and number of eggs, galls, egg masses and reproduction factor decreased by 33, 18, 18, and 20%, respectively. All elicitors increased the expression of PR1 and NCED genes in nematode infected tomato. These data suggest that SA, BABA and ABA activate similar defenses in tomato plants, which is partly SA- and ABA-related. SA, BABA, and ABA pretreated tomatoes infected with M. incognita trigger a SAR-response and lead to the control of the nematode under controlled conditions.
Volume 26, Issue 1 (1-2024)
Abstract
Induced resistance in plants can be manipulated using the application of elicitors. In this study, the effects of Salicylic Acid (SA), chitosan, γ-Aminobutyric Acid (GABA), Serenad Aso (Bacillus subtilis QST713), and combined application of these elicitors were studied on the resistance of canola to cabbage aphid, Brevicoryne brassicae L. (Hemiptera: Aphididae). The number of B. brassicae attracted to canola plants treated with different inducers was significantly lower compared to the control in the field experiments. The lowest population density of aphid was observed on plants treated with SA+GABA and SA+chitosan. Moreover, the highest amounts of glucosinolates in canola leaves were recorded in SA+GABA treatment. The contents of sugar, lipid, and glycogen of B. brassicae were lowest when fed on the plants treated with SA integrated with GABA or chitosan. Furthermore, the activity of catalase, esterase, peroxidase and glutathione S-transferase enzymes was the lowest in SA+GABA and SA+chitosan. These findings suggest that exogenous application of SA integrated with GABA or chitosan on canola plants are successful in suppressing the B. brassicae population and can be considered in integrated management programs against this and other aphids.
