Showing 4 results for Total Protein
P. Fathi Rezaei, E. Rakee,
Volume 10, Issue 3 (9-2019)
Abstract
Datura (Datura stramonium) is known as a rich source of tropane alkaloids, including scopolamine and hyoscyamine as parasympatholytics that competitively antagonize acetylcholine. Production of secondary metabolites often occurs in plants against the various elicitors or signal molecules. In this study, the effect of yeast extract as a biotic elicitor on tropane alkaloid production was evaluated. Surface-sterilized Datura seeds were cultured on 1/2 MS medium supplemented with different concentrations of yeast extract (0, 1.5, and 3g/L). One month after germination, alkaloid yield of Datura plantlets was measured by the use of High-Performance Liquid Chromatography (HPLC). Also, total protein content and antioxidative enzymes activity were determined by spectrophotometry method. According to the results, the fresh weight of root and shoot parts of Datura plantlets at 1.5g/L yeast extract was increased about 2 and 4 times, respectively. Yeast extract (1.5g/L) caused to 1.7 times increase of hyoscyamine amount of root and shoot parts and 2.5 folds scopolamine of Datura plantlets. Moreover, on yeast extract (1.5g/L)-treated plantlets, total protein content, and activity of catalase and guaiacol peroxidase were almost the same as the control group. Altogether, yeast extract (1.5g/L) can be used as a good candidate for enhancement production of tropane alkaloids especially hyoscyamine and scopolamine with high medicinal value.
Volume 14, Issue 2 (3-2012)
Abstract
Wheat Streak Mosaic Virus (WSMV) causes extensive economic damage to wheat (Triticum aestivum L.) in many regions of the world. The present study was conducted to find out if the variations in biochemical changes in reaction to WSMV infection in F2 generation of either potentially resistant (Adl-Cross) or susceptible (Marvdasht) cultivars are genetically inherited. A factorial experiment was employed with two factors of: genotypes (Adl-Cross, Marvdasht, reistant F2s and susceptible F2s), and inoculation (either infected or non infected) at Shiraz University, Iran during 2007-2009. Leaves of seedlings were harvested at different time intervals for total protein, total phenolic compounds and peroxidase activity analysis. Results indicated that virus infection caused stress in all genotypes. Total protein reduction in the inoculated resistant Adl-Cross and in its F2s was not significant whereas it was significant in the inoculated susceptible Marvdasht and its susceptible F2s. Viral infection reduced peroxidase activity in the susceptible Marvdasht cultivar and in its susceptible F2s whereas in Adl-Cross and in its resistant F2s the activity was increased. It is speculated that peroxidase enzyme may affect synthesis of compounds effective in resistance to wheat streak mosaic virus. The trend in the increase in phenolic compounds indicated that their formation and accumulation is faster in the resistant genotypes as compared with the susceptible ones. It appears that the extent of total protein, total phenolic compounds as well as peroxidase activity changes in response to WSMV are inherited by the next generations and these biochemical changes in a genotype could be adopted as selective factors in the preliminary experimental stages of selection for tolerance to the virus.
Volume 16, Issue 91 (9-2019)
Abstract
The objective of this study was to evaluate the effects of pasteurization temperatures and cold shelf storage on chemical, microbial, and quality characteristics functional milk-carrot drinks. For this purpose, milk-carrot drinks based on cow milk were prepared with different concentration of carrot juice (10, 20, 30, 40, and 50%) and pasteurized at 65, 70, and 75 °C for 30 min. Physicochemical characteristic such as total acidity, pH, Sugar (total sugar, reducing sugar, and non-reducing sugar), total protein, coliform, pasteurization efficiency, stability, phase separation, and sensory parameters were measured during storage times (1, 4, 7, 10, and 13 d) at 4 °C. The results indicated that total acidity and pH of milk-carrot samples pasteurized at 70 °C had lower variation than other treatments during cold shelf. The highest non-reducing sugar content was belong to 50% carrot juice (75 °C) at 13 d and the highest reducing sugars was observed at 4 d for all samples in pasteurization temperatures were. Pasteurization temperatures and storage time had not significant effect on total protein of samples with different carrot juice content. Pasteurization efficiency test showed that all of the pasteurization temperature-time treatments could completely inactivated alkaline phosphatase enzyme. But in coliform test, samples with 50% carrot juice (65 °C) at 13 d and 30% carrot juice (75 °C) at 13 d showed 3 and 12 colonies respectively. Stability test and phase separation indicated that all treatments during cold shelf had necessary stability. Sensory evaluation determined that samples containing 40 and 50% carrot juice had the highest total acceptation. I t could be concluded that the optimum conditions for functional milk-carrot drink formulation, pasteurization and storage time for milk-carrot drink were 40 and 50% carrot juice substitution, 70 °C pasteurization and 10 d cold shelf stability respectively.
Volume 18, Issue 4 (7-2016)
Abstract
In this research, two field crops(Zea mays L. and Phaseolus vulgaris L.), two medicinal plants(Hyssopus officinalis L. and Nigella sativa L.)and two weeds(Amaranthus retroflexus L. and Taraxacum officinale F. H. Wigg) were separately treated with three concentrations of SiO2 nanoparticles (400, 2,000, and 4,000 mg L-1). The effects of these treatments on morphological and biochemical characteristics of the plants were assessed, including germination, root and shoot length, root and shoot fresh weight, root and shoot dry weight, photosynthetic pigments, total carbohydrates, total protein, total amino acid, and proline content. In the crops and medicinal plants, 400 mg L-1 SiO2 NPs significantly increased seed germination, root and shoot lengths, fresh weights (except for H. officinalis) and dry weights, photosynthetic pigments, total protein, and total amino acid (except for H. officinalis). In weeds, as SiO2 NP concentration increased from 400 to 4,000 mg L-1, germination, root and shoot lengths, fresh and dry weights, and photosynthetic pigments as well as total protein decreased. Total carbohydrates in all plants decreased significantly, except for A. retroflexus at 400 mg L-1 SiO2 NPs. In all plant species, with increasing SiO2 NP concentration, proline content increased significantly. According to these results, a lower concentration of SiO2 NPs can have beneficial effects on morphological, physiological, and biochemical characteristics of plants.
