Showing 6 results for Transcription Factor
Ebrahim Mahmoudi, ,
Volume 5, Issue 1 (11-2014)
Abstract
One of the largest gene families in plants is MYB which contain a DNA-binding domain regulating gene transcription. These transcription factors (TF) have various functions in a wide range of plants. For example, biosynthesis pathway of anthocyanin as secondary metabolites and responsible for coloration in different plant tissues is regulated by the above-mentioned TFs. In the current study, Using homologous genes in the species close to mulberry (Morus Alba), a fragment of a new MYB transcription factor belonging to MYB gene family was isolated from this plant. First protein sequence of 14 different genes regulating anthocyanin from the species close to mulberry family (moraceae) were selected and then aligned, subsequently the region with the highest similarity was nominated. Following this, the cDNA sequence of these genes was aligned and a location which had a high rate of similarity was considered. With regard to large polymorphism in this sequence, degererate primers were designed and used to isolate the target gene.
I. Yousefi Javan , F. Gharari ,
Volume 9, Issue 4 (12-2018)
Abstract
Aims: Osmotic stress such as drought, salinity, and cold is one of the most important stresses. The aim of this study was to isolate and evaluate the genes of AREB and MPK2 in order to study the resistance to drought of tomato plants.
Materials and Methods: In this experimental study, seeds of two varieties of Tomato (Red Cloud) and (Peto Pride; resistant and susceptible to drought stress, respectively) were grown in drought treatment levels of -2 and -4. This study used 3 replications by a model based on a completely randomized block design. Sampling was done for Thiobarbituric acid reactive material (TBARM) for each treatment in 3 replications. Randomized and repeated sampling were done for molecular studies and the genes expression. AREB1 and MPK2 genes were studied, using bioinformatics resources and with the help of specific primers, making cDNA, PCR, and Electrophoresis. The analysis of variance test and SPSS 15 software were used
Findings: With increasing drought stress, most of morphological traits had a considerable decline, but cellular oxidative index increased with the increase of stress, so that TBARM increased. The expression of AREB1 was higher than that of MPK2 gene expression. The rate of similarity between LeAREB and kinase 2 protein sequences in resistant tomatoes was 31%.
Conclusion: With increasing drought stress, most morphological traits have a significant decline, but TBARM shows a significant increase with increasing stress. The AREB1 resistant drought gene is induced by the effects of drought stresses, while the expression of the MPK2 gene does not show a significant difference.
Leila Pourhang, Zahra Ghorbanzadeh, Mehrbanoo Kazemi Alamuti, Seyyed Mohammad Mousavi Pakzad, Elahe Moatamed, Mona Mapar, Aliakbar Ebadi, Mohammad Reza Ghaffari*, Katayoun Zamani, Ghasem Hosseini Salekdeh, Behzad Ghareyazie, Motahhareh Mohsenpour,
Volume 14, Issue 1 (3-2023)
Abstract
Production of drought tolerant crop is an important strategy for avoiding water scarce crisis. Improvement of the root structure leading to the higher yield and seed quality. In this study, three genes affecting root structure, drought tolerance and phosphorous absorbance are used in producing hybrid constructs used for the rice transformation. Three genes: a serine/threonine protein kinase (PSTOL1), a gene from the cytokinin oxidase/dehydrogenase family (OsCKX4) and a transcription factor induced under stress from the NAM-ATAF-CUC family (OsNAC5) isolated from the rice wild cultivars are cloned under separate regulatory elements in the T-DNA region of the Agrobacterium binary vector. OsNAC5 gene was cloned under RCc3 root specific promoter and PSTOL1 gene under ubiquitin promoter. Also, OsCKX4 gene was cloned once under ubiquitin promoter and once under RCc3 promoter. Two hybrid multi-gene constructs named pUhrN5CkPstol and pUhrCkPstol harboring multiple genes are synthetized and used for the gene transformation into the Hashemi cultivar. Gene transfer was done to callus obtained from mature rice seeds. Transgenic plants were confirmed using PCR analysis. From the number of 107 regenerated plants in which the presence of transgenes was proved, 14 transgenic events were finally obtained. Root structure of the T0 plants showed drastic phenotypic difference in comparison to the non-transgenic ones. By now, one transgenic event harboring CKX4 and PSTOL1 is confirmed to have a homozygous line in T2 generation. It is hoped that genetic engineering of rice for enhanced root structure lead to drought tolerance, reduce water consumption and improve yield under stress conditions.
Volume 14, Issue 5 (9-2012)
Abstract
Interleukin-1β (IL-1β), the prototypical pro-inflammatory cytokine, is produced by macrophages following exposure to bacterial products. Its role is to act upon several cell types at the site of infection to stimulate the production of pro-inflammatory molecules that will cause increase in vascular permeability. Therefore, IL-1β regulates the initiation and development of acute inflammation that may have a role in mammary gland defense during mastitis. Single nucleotide polymorphisms (SNPs) in the 5'-flanking region of this gene can modulate IL-1β function. The aim of the present study was to discover and analyze SNPs in promoter region of IL-1B gene in cattle (Bos taurus). The 5'-flanking region of IL-1B gene was screened by single strand conformation polymorphism (SSCP) in Holstein and Iranian local cattle breeds (50 local and 50Holstein). A total of 4 distinct SSCP patterns were observed, which further revealed 5 novel SNPs upon sequence analysis in Iranian local breed. From the SNPs identified in this region, polymorphism at nucleotide position -534 was found to lie in the vicinity of potential GATA and ZNF transcription factor binding sites. The SNPs identified at -383 position was shown to be present within the putative ETS factor and also core sequence of CARE transcription factor. Two SNPs at positions -534 and -340 were found within the EBF binding site. The SNPs identified in the 5'-flanking region of IL-1B gene may serve as potential candidate genetic marker(s) for disease resistant traits in cattle.
Volume 16, Issue 3 (5-2014)
Abstract
The Melanocortin-1 Receptor MC1R is encoded by the extension locus, playing a fundamental role in the determination of coat color in a number of mammalian species. However, so far there has been no report regarding the Single Nucleotide Polymorphisms (SNPs) of the MC1R promoter region and the potential association of its SNPs with coat color in sheep (Ovis aries). Throughout the present study, the promoter region of the MC1R gene was screened using Single Strand Conformation Polymorphism SSCP and DNA sequencing in the Karakul breed of sheep. A total of 4 distinct SSCP patterns were observed which revealed 3 novel SNPs and an insertion/deletion of 26 nucleotides upon sequence analysis in the analyzed population. In silico analysis of the MC1R promoter sequence predicted no consensus TATA-box motif at an appropriate position but detected multiple putative transcription factor binding sites for Ets, AML-1a , NF-E2, MZF1, USF, Oct-1 and GATA-1. The analysis of identified polymorphic sites also showed that the polymorphism at nucleotide position -89 relative to the start codon abolishes the USF transcription factor binding site. The SNP identified at the -100 position is located within a putative AML-1a transcription factor binding site. The insertion of 26 nucleotides at position -126 made a putative binding site for the MOK2 transcription factor. The possible functional activity of the identified genetic variations could be confirmed using gene expression analysis.
Volume 23, Issue 2 (3-2021)
Abstract
The objective of this work was to understand the mechanisms of physiological, biochemical, and molecular responses to salinity stress of three Turkish melon genotypes (YYU 1, YYU 4 and CU 196) and cv. Ananas. The study used Randomized Complete Block Design (RCBD) and pots were irrigated with Hoagland nutrient solution after two-leaf stage until harvesting by 50 and 75 mM NaCl concentrations. For evaluation of responses, chlorophyll and carotenoid content, total phenolic and flavonoid amount, proline variations, and nutrient elements were determined. Moreover, qRT-PCR analyses were performed to identify the expression level of six TF (Transcription Factors) genes (WRKY24, TCP15, CmHD-Zip, mTERF2, Dof3 and CmADH2). Increase in salt application led to increase in chlorophyll content in the melon genotypes, but decrease (about 55%) in cv. Ananas. Phenolic, flavonoid, and proline contents varied based on the melon genotypes, but generally increased in Ananas. Expression levels of TCP15 and WRKY24 showed more fold change at 75 mM NaCl treatment. On the other hand, the expression of CmADH2 and Dof3 showed more fold change at 50 mM NaCl treatment. Finally, according to adaptation mechanisms of melon genotypes, the study might help in selection and detection of the salt tolerant ones.
