Showing 7 results for Vascular Endothelial Growth Factor
Volume 1, Issue 2 (7-2014)
Abstract
Background: Henoch-Schönlein purpura (HSP) is an lgA mediated small vessel systemic vasculitis disease in children. The etiology and pathogenesis of HSP disease remain unknown. However, environmental and genetic risk factors could play important roles in susceptibility to HSP disease. In this study we investigated the association of 5՛-untranslated region polymorphism (-634G/C) of VEGF gene with HSP among Iranian Azeri Turkish population. Methods: Thirty unrelated Iranian Azeri Turkish children with HSP and fifty healthy unrelated subjects without HSP and other inflammatory diseases were enrolled in this population. -634G/C polymorphism of VEGF gene was genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique. Results: The distribution of CC genotype in VEGF -634G/C polymorphism statistically showed a significant difference in HSP patients in compare to that of control group (P= 0.009). Conclusions: The CC genotype of VEGF -634G/C polymorphism could be associated with susceptibility to HSP disease in Iranian Azeri Turkish ethnic group.
S. Shirin Shahangian, Shirin Jalili, Ammar Mohseni, Reza Hassan Sajedi, Sadegh Hasannia, Majid Taghdir, Mohammad Mohammadi, Rayhaneh Sariri,
Volume 5, Issue 1 (11-2014)
Abstract
Regarding the importance of inhibiting VEGF and unique features of VHHs as a new generation of antibody-based therapeutics, the present study aimed to generate VHHs against the receptor binding domain of VEGF, thereby blocking of VEGF binding to its receptor. After preparing the gene repertoire of VHH fragments from an immunized camel, a VHH phage display library was constructed. We adopted a stringent successive biopanning to isolate the phages displaying VHH with high affinity to VEGF-RBD.A significant enrichment of phages that specifically bound to the target protein was obtained after six rounds of panning. Of the specific clones with high binding affinity screened by monoclonal phage ELISA, 52% shared the same VHH sequence, showing its high enrichment. Using molecular simulation of antigen-antibody interaction based on the crystallographic information of VEGF/VEGFR2, molecular dynamics simulations and MM/PBSA free energy calculations, we provide a reliable picture of the binding site of antibody on antigen. The key residues in the VEvhh1-VEGF interface were dissected and the energetics was analyzed by MM/PBSA. The results of studies revealed that VEvhh1 binds to the receptor binding site of VEGF with high binding energy and showed the highest affinity to the residues of VEGF which are responsible for VEGF binding to VEGFR2. Also the antibody potently covers these key functional residues of VEGF, thereby inhibiting VEGF binding to its receptor and probably abrogating its biological activity. This study may represent VEvhh1 as an anti-VEGF and anti-angiogenic candidate.
Vahid Zeraati, Parviz Abdolmaleki, Reza Hassan Sajedi, Abdolkarim Moazeni-Roodi,
Volume 13, Issue 1 (3-2022)
Abstract
Investigation of factors affecting endothelial cell proliferation is an essential part of angiogenesis studies. Given the importance of inhibiting angiogenesis in the treatment of cancers, and due to the side effects and high cost of anti-angiogenic drugs such as Avastin, the use of physical agents to aid in treatment and reduce the need for high doses of the drug is noteworthy. Magnetic fields are of interest due to their long-distance and non-invasive effects, and many studies have been conducted on their effects on biological phenomena, including angiogenesis, with inconsistent results. In the present study, the effect of a 2 mT alternating magnetic field with a frequency of 200 Hz and Austin on the proliferation of human umbilical vein endothelial cells (HUVEC) was investigated. Cells were treated for 48 hours under a mixture of 50 μg/ml solution of vascular endothelial growth factor (VEGEF) and Avastin at concentrations (zero (drug control), 50, 100, 200 and 400 μg/ml) as well as field treatment groups for They were exposed to magnetic fields for 3, 6, 12, 24 and 48 hours. Then, cell proliferation was assessed using Alamar Blue colorimetric test. Data were analyzed by three-way analysis of variance. According to the findings, the exposure times of 12, 24 and 48 hours showed a significant reduction in cell proliferation compared to the control group, but this difference was not significant in the 3 and 6 hour treatments. Also, the degree of interaction of these factors with each other on HUVEC proliferation was investigated.
Salim Alhafyan, Zeynab Rezaei, S.s. Shahangian, Reza H. Sajedi,
Volume 14, Issue 2 (5-2023)
Abstract
Angiogenesis is involved in many physiological and pathological processes, including tumor growth, and VEGF is considered as the most important factor in this process. Nowadays, the production of single-domain antibodies (VHH) with the characteristic of inhibiting growth factors in cancer tumors is one of the new strategies for cancer treatment. In the previous research, it was found that camel VHHs isolated from phage display against VEGF play an essential role in inhibiting it. Here, the VHH that had the highest affinity for the VEGF was selected. Ice nucleation protein (INP) was used as anchoring motif for surface expression of E. coli, owing to the efficiency of its N terminal domain, this system is used to express the VHH. Accordingly, a construct harboring the first 537 nucleotide of InaK gene and nucleotide fragments of TEV protease recognition site and VEvhh10 was designed to express this protein in the surface display of E. coli cells. The results showed that the INP anchor is a suitable candidate for promoting the surface expression of VEvhh10 in E. coli. After expression of VEvhh10, isolation and purification were performed using centrifugation and washing, and its binding to VEGF was investigated. The results showed that VEvhh10 successful bind to VEGF and it can be used for therapeutic applications and clinical diagnosis of patients in the future.
Volume 21, Issue 1 (4-2018)
Abstract
Aims: Exercise activity and the use of herbal supplements are effective in regulating vascular contraction by influencing on angiogenesis factors. The purpose of this study was to investigate the effect of caffeine and genistein supplement on cardiac levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in wistar rats, following an acute swimming exercise.
Materials and Methods: In this experimental study, 49 male Wistar 8-10-week rats were randomly divided into 7 groups of control, saline, acute exercise, caffeine, genistein, acute swimming exercise plus caffeine, and acute swimming exercises plus genistein (7 rats in each group). Acute exercise included 90 minutes swimming. Dietary supplement groups, taked the caffeine at a rate of 5mg/kg of body weight by gavage and Genistein in the amount of 100mg/kg of body weight by intra peritoneal injection. Two hours after the end of the training session, the levels of NO and VEGF were measured by ELISA. Data were analyzed by SPSS 20 software using one-way ANOVA.
Findings: 90 minutes swimming exercise caused a significant increase in cardiac levels of NO and VEGF in acute exercise, acute exercise plus caffeine, acute exercise plus genistein in comparison to the control and saline groups (p=0.01), while supplementation of caffeine or genistein alone did not significantly increase the levels of cardiac level of these factors compared to control and saline groups.
Conclusion: The supplementation of caffeine and genistein increases the levels of NO and VEGF of the heart tissue of rats following an acute swimming session.
Volume 21, Issue 3 (10-2018)
Abstract
Aims: Vascular disorder is one of the consequences of diabetes. Physical activity also plays an important role in the improvement of this disorder. Therefore, the aim of this study was to investigate the effect of vigorous aerobic training on serum levels of inhibitory and excitatory factors of Angiogenesis in women with type 2 diabetes.
Materials and Methods: In this quasi-experimental study with pre-test and post-test design that was performed among women with diabetes population in Gorgan in volunteer sampling, 28 women with type 2 diabetes in Gorgan were selected By … sampling method and were divided into training and control groups (14 individuals each group). Training intervention consisted 8 weeks of vigorous aerobic exercise (70%-80%) with 3 times per week. Just before the exercise and 48 hours after that, serum levels of Nitric Oxide (NO), Vascular Endothelial Growth Factor (VEGF), and Endostatin (ES) were evaluated. The data were analyzed by SPSS24 software, using repeated measures ANOVA..
Findings: Eight weeks of aerobic training caused a significant increase in serum levels of NO (p=0.016), VEGF (p=0.036), and an insignificant increase in ES (p=0.237). Also, a significant decrease was observed in fasting blood glucose (p=0.027) and A1C (p=0.003) compared to the control group.
Conclusion: Regular aerobic exercise improves serum levels of angiogenesis-related and metabolic factors in women with type 2 diabetes.
Volume 21, Issue 3 (10-2018)
Abstract
Aims: The aging process is associated with alterations in brain neuroangiogenic factors. Exercise training plays an important role in the development of brain, especially in the elderly. The aim of this study was to investigate the effect of high-intensity interval training on some of the brain neuroangiogenic factors of old female rats.
Materials and Methods: The present experimental research was carried out on 14 two-year-old female Wistar rats, weighing 250-300 grams. The rats were randomly divided into 2 control and exercise groups (7 rats per group). The old animals performed 10 bouts of 1-min high-intensity treadmill running (20–34 m/min), separated by 2 minutes of rest 3 days a week for 8 weeks. The rats were killed 48 hours after the last exercise training session and brain vascular endothelial growth factor (VEGF) and transforming growth factor‐β1 (TGF‐β1) levels were evaluated by ELIZA method. The data were analyzed by SPSS 20, using independent T-test.
Findings: VEGF levels in the exercise group were significantly higher in the brain tissue of old rats than in the control group. TGF-β1 brain levels decreased compared to the control group.
Conclusion: High-intensity interval training improves age-induced neuroangiogenic changes in the aged brain via the up-regulation of VEGF and decrease in TGF‐β1 level.
