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Showing 2 results for Magnetosome
Abbas Sepahi, , ,
Volume 6, Issue 1 (10-2015)
Abstract
Magnetotactic bacteria can be affected by Earth's magnetic field orientation. They have been observed various forms of cocci, bacilli and spirilla. These bacteria make magnetosomes affected by the magnetic field and its orientation as part of the iron is stored. In this study, the presence of magnetotactic bacteria in different parts of Iran with features freshwater pearls saline containing iron mine in Zanjan, Persian Gulf, Mighan and Qom wetlands was investigated. Sediments and water collected were put under magnetic field. Results showed that all types of isolated bacteria were, Gram-negative and shapes to bacillus, spirillum and coccus. The magnetic field in the capillary tube containing a magnetotactic bacterium that responds was observed to magnetic field. Mighan and Qom wetlands and Zanjan's iron mine samples have more cases of magnetotactic bacteria,were studied in particular. Transmission electron microscopy images of iron nanoparticles were visible within it. Results molecular analysis, sequencing and BLAST in NCBI website showed in sample Znjan's iron mine there is Magnetospirillum magnetotacticum strain. Higher concentrations indicate of iron nanoparticles in the sample compared to the Mighan wetland with iron mine, that the abundance of iron ions alone did not increase the level of magnetic nanoparticles of Fe by bacteria.
S.m.r. Aghamiri , S. Akbari-Karadeh , P. Tajer Mohammad Ghazvini , S. Ghorbanzadeh Mashkani ,
Volume 9, Issue 2 (9-2018)
Abstract
Aims: In recent years, magnetotactic bacteria and their magnetic nanoparticles (magnetosomes) were considered in different fields of science, including medicine, biotechnology, and nanobiotechnology due to their novel and unique magnetic properties. The present study was performed with the aim of evaluating the effect of temperature and reducing agent on labeling of magnetosomes with 188Re and biodistribution of labeled magnetic nanoparticles.
Materials and Methods: In this experimental study, Alphaproteobacterium MTB-KTN90 and sonication extraction method were used for the extraction of magnetic nanoparticles. After bacterial lysis, the magnetic nanoparticles produced by electron microscope were investigated and tin (II) chloride, as reducing agent, was used to check the labeling efficiency and rats were used to examine the biodistribution of the labeled magnetosomes.
Findings: The highest efficiency in magnetosome labeling experiments was 11100kBq in the initial activity, which decreased with increasing activity. The increase in temperature did not have much effect on increasing the labeling efficiency. The labeling value in the absence of a reducing agent was 721.5kBq, while at a concentration of 2mg of this agent, the labeling value increased to 10745.91kBq. After the injection of magnetosomes through the sublingual vein of the rat, the magnetosomes accumulated in the liver.
Conclusion: Magnetosomes extracted from Alphaproteobacterium MTB-KTN90 have a high potential for labeling by 188Re. Increasing temperature does not affect the labeling efficiency, but tin (II) chloride is a very important factor in optimizing the labeling efficiency, and the highest accumulation of magnetosomes labeled with 188Re after injection is in the liver of the rat.
