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Showing 2 results for Podophyllotoxin
, Mozafar Sharifi, Mehrdad Behmanesh,
Volume 4, Issue 1 (10-2013)
Abstract
Linum album is an herbaceous and medicinal plant that has important lignan such as podophyllotoxin (PTOX). PTOX has antiviral and anticancer properties. Since the chemical synthesis of PTOX is an expensive process, production of PTOX using cell and cultures of linum species is a cost-effective alternative approach. Various strategies have been employed to increase the production of secondary metabolites in cell cultures. In this study, we have verified the effect of chitosan on cell growth, PTOX production in 1, 2, 3 and 5 days after treatment. Cells elicited with chitosan for 5 days yielded the highest amount of PTOX. To study mechanism of chitosan action, expression of phenylalanine ammonio-lyase (PAL), cinnamoyl-CoA reductase (CCR), cinnamyl alcohol dehydrogenase (CAD) and pinoresinol lariciresinol reductase (PLR) genes were investigated. The expression of genes were increased, reaching a peak at 3 day after treatment. Chitosan up-regulate the production of PTOX, by effecting on gene expression of PTOX biosynthesis pathway.
Parvaneh Peyvand, Zahra Vaezi, Hossein Naderi-Manesh,
Volume 16, Issue 1 (12-2024)
Abstract
Hepatocellular carcinoma, predominant form of liver cancer, is the main cause of death in patients with liver cirrhosis. Podophyllotoxin, a natural anticancer compound, has ideal anti-tumor properties. However, its use is limited due to poor solubility and bioavailability. Finding a suitable drug delivery system have great importance in improving the bioavailability of podophyllotoxin. In this study, mPEG-PCL nanoparticles have been used for delivery of podophyllotoxin to liver cancer cells. mPEG-PCL copolymers were synthesized and characterized by DLS, FTIR and NMR analyses methods. The critical micellization concentration was 0.055 µg/ml. The z-average and surface charge of micelle was 186 ± 12 nm and -5.13 mV, respectively. podophyllotoxin was loaded in micelles in different w/w ratios of drug: copolymer. The size of the nanodrug was 214 ± 20 nm and the weight ratio of 1:1 with encapsulation efficiency of 77.36 ± 1.23 % was selected as the optimal ratio. The drug release results showed a significant difference between the rapid release of free podophyllotoxin and the more stable release of the loaded drug. At 37°C, drug release was higher, which was attributed to the destruction of polymersome structure at this temperature. According to the cytotoxicity study, the IC50 value for nanodrug (8.64 μg/ml) was lower than the IC50 value for the free drug (12.79 μg/ml), which showed the effect of improved cytotoxicity of nanodrug compared to the free drug. The results proved the polymersome can be potential carriers for delivery, controllable release and improve the toxicity effect of podophyllotoxin in cancer chemotherapy.
