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Probiotics are live microbial feed supplements, which beneficially affect the host by improving its intestinal microbial balance[8].Recently, researches have shown that lactic Acid Bacteria(LAB), especially Bacillus (because of having endospore) remain stable at cooking temperature and retain their probiotic benefits in baked goods [3]. The purposeof this study,was using of Bacillus coagolans, as a resistant probiotic,for enrichment of  bread. First, these probiotic bacteria were determined by the tests of salt tolerance, heat resistance, bile tolerance, tolerance of acid and pepcin, resistance of antibiotics, and preventing the growth of pathogenic starins[18].Then a certain number cells of  BC were entered in bread dough,before& after  baking the number of live bacteria were calculated by colony count in 1g of dough and bread. The number of BC decreased from 10⁸ to 10⁶ units per gram, after baking. Also,the amount of starch decreasd and changed into simple sugers. The pH was estimated about 4.5-5 and TTA (Total Titritable Acidity) was between 6-8. Finally, the enrichment of bread was  evaluated  by experts and its quality and taste were compared with a control sample. The Results showed this bacterium survives in baked bread and makes good chemical changes on it.

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The effect of three probiotics (Lactobacillus plantarum, L. casei and Bacillus subtilis) on growth and survival of artemia against a pathogenic bacteria, Vibrio campbellii, in gnotobiotic conditions were investigated. A total of 1560 nauplii were equally transferred to 78 falcon tube containing 30 ml of autoclaved sea water with 70 ppt salinity. Treatments consisted of a control (alga, Dunaliella tertiolecta) and 12 treatments of 3 bacteria (each with 25%, 50%, 75%, and 100% replacement) and each treatment was done in 6 replicates. On the sixth day, survival rate, length and the number of bacterial colonies in the nauplius were studied. Also, in this day the nauplius was challenged with the pathogenic bacteria, V. campbellii. The maximum length of the nauplius was observed in the group supplied with 25% L. plantarum and 75% of alga (P<0.05). The results of bacterial colonies showed significant differences between groups (P<0.05). The highest and lowest number of colony were shown in groups supplied with 100% B. subtilis and 25 % L. casei, respectively. Based on challenge results, after 24 and 48 h of challenging with the pathogenic bacteria, the groups supplied with 100% L. plantarum showed the highest survival rate and the lowest survival was in group fed with 100% alga. In conclusion, the use of 25% L. plantarum combined with algae showed a better performance than the L. casei and B. subtilis.

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Introduction: Since the gastrointestinal system plays an important role in the function of the immune system, its role in the control or treatment of autoimmune diseases cannot be ignored. Therefore, the intestinal strengthening, which much of gastrointestinal function depends on it, can be effective in this direction. Also, because the intestine plays an important role in the immune system in addition to digestion, it can help maintain the immune system's function by keeping its bacteria balanced. In this regard, probiotics and prebiotics can be useful, which this issue was investigated in the present study.
Conclusion: Probiotics have an important role in the prevention and control of multiple sclerosis.
 

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Because of their positive effect, probiotic bacteria widely used in the manufacture of food, particularly in the production of dairy products. but having high cholesterol and lactose intolerance problem is an important restrictions on the use of dairy products. Recently, the consumption of non-dairy probiotic foods specifically non-dairy probiotic juices were developed. The aim of this study was to examine the growth trend of Lactobacillus acidophilus in the two varieties of Pomegranate juice Shahreza and Natanz. After inoculation of bacteria to each samples, they were stored at 4 ° C and changes in pH, titratable acidity, the sediment, as well as live cell counts were measured under controlled conditions at distinct times during fermentation. The results showed that there was lower survival of bacteria in Natanz pomegranate juice due to the lower pH. The microbial populations during the first week was decreased from 7.43 to 4.5 logarithmic cycles, While microbial populations of Shahreza pomegranate juice was decreased from 7.49 to 5.33 logarithmic cycle at the same time. In the second week of research, there was no bacteria detected in both treatments. Both treatments showed a significant decrease in pH, sediment and bacteria population at the end of maintenance, but the changes in acidity were not significant (p˂0.01). There was no desirable effect on sensory properties of pomegranate juice after adding probiotics Bacteria

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Reducing of salt content in cheese production is an important challenge for the dairy industry. Based on consumer demand for functional and healthy food, in this study, the feasibility of salt reduction in ultrafiltered white probiotic cheese was studied. For this purpose, white probiotic cheese was prepared with different levels of salt (2, 3 and 4%) with two probiotic strains (Lactobacillus acidophilus and Bifidobacteriumlactis) using response surface methodology and central composite design, and evaluated during 60 days storage time. Fitted models showed a high correlation coefficient to predict selected response variables including chemical and sensory characteristics, survival of probiotic bacteria and textural properties in UF-cheese. ANOVA results showed that change in salt concentration had a significant effect on physicochemical characteristics, texture parameters and overall acceptance (P<0.05), while the survival of probiotic bacteria was unaffected. According to the obtained results, by increasing the salt concentration in cheese the syneresis and dry matter, as well as the hardness of the samples increased, while the acidity and cohesiveness were decreased. Viability of probiotic bacteria was affected by storage time, so that the number of Lactobacillus acidophilus in low salt probiotic cheese and at the end of storage period was more than Bifidobacterium lactis. Lowering the salt content of probiotic cheese led to a lower sensory acceptability. Optimum conditions to produce low salt probiotic cheese through Bifidobacterium lactis and Lactobacillus acidophilus were obtained 2.61% salt ( 18 days storage) and 2.49% salt (60 days storage), respectively.

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For many years, human tend to use fermentation products due to the presence of nutrients that are effective on health. The health effects of the Functionl fermentation products are or directly due to the presence of microorganisms, and their function in the host body or indirectly the result of their metabolites during fermentation. Cereal-based products have suitable bioavailability for probiotic cells and furthmore they have desirable sensory and nutritional properties. The purpose of this study was to use probiotic bacteria and yeast alone and also simultaneously at three levels of wheat sprout flour to prepare a functional beverage. The study evalated the changes of pH , acidity , counting of probiotic cells and folic acid content . furthermore , the sensory characteristics were checked using a 5-point Hedonic test. Data were analyzed by SPSS software. Results showed that acidity increased with increasing flour content. The highest survival of probiotic beverage containing 10 grams of wheat sprout flour with bacteria and yeast Initiation culture. in terms of the sensory characteristics and folic acid, the drink contains 15 sprout of flour with bacteria and yeast.

 

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Free radicals are a major cause of many diseases, such as cancer, by damage to the cells. The use of probiotic strains in fermented dairy foods has expanded due to the health-promoting effects of the consumer. Regarding antioxidant potentials of probiotic bacteria, the aims of this study compared the antioxidant activity of probiotic species L. acidophilus LA5 and B. animalis subsp. lactis BB12 used in foods and investigate effects of incubation temperature, Initial pH, fermentation time, yeast extract concentration and linoleic acid concentration on their antioxidant activity in enriched cheese whey and milk permeate. The results showed that fermentation time, incubation temperature and concentration of yeast extract were the most important factors that had a significant effect on both DPPH free radical inhibitory activity and hydroxyl radical scavenging activity (p <0.05). DPPH free radical inhibitory activity and hydroxyl radical scavenging activity increased with increasing incubation temperature and yeast extract concentration, respectively. Antioxidant activity was observed in the first 24 hours of the fermentation process, which was proportional to bacterial growth. Hydroxyl radical scavenging activity and DPPH free radical scavenging activity, as a result of the probiotic culture activity and their effect on the substrate, increased and decreased, respectively, in the first 24 hours of fermentation time, by the destruction the polymers. This study showed that the fermentation bioprocess by L. acidophilus LA5 and B. animalis subsp. lactis BB12 in the cheese whey as a medium had high antioxidant activity.

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In this study, the milk-derived GalactoOligoSaccharides (GOS) were produced by Lactobacillus delbrueckii subsp. bulgaricus and refined by an ultrafiltration-nanofiltration continuous membrane. By further investigation, we found that the GOS product purified by gel permeation chromatography mainly contained low molecular weight disaccharide and trisaccharide, that is, 4-β-galactobiose and tri-galacto-oligosaccharides. The cellular immune activity of the purified GOS was evaluated by using Intestinal Epithelial Cells (IECs). Results showed that GOS could significantly (P< 0.05) promote IECs proliferation in a dose and time dependent manner, and the relative proliferation rate after 24 hours culture was high up to 158% at the concentration of 100 μg mL^-1, which was three time the value after 4 hours culture without GOS. Moreover, the production of IL-6 was observably increased and up to 133.54 ng L^-1 with addition of 100 μg mL^-1 GOS. These data implied that the purified GOS might have a role in promoting the immune adjustment, which could be utilized as a novel and natural immunoregulatory agent in the field of medicine and functional food. This work also revealed that the employment of transgalactosylation activity of β-galactosidase derived from the fermentation of probiotics such as Lactobacillus delbrueckii subsp. bulgaricus would enhance the value of the milk product due to the form of GOS.

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In this research, the production of a probiotic drink based on jujube extract by means of ‎fermentation with Lactobacillus plantarum and Lactobacillus delbrueckii as probiotic ‎lactic acid bacteria has been studied. The fermentation was performed for 72 hours at ‎‎37°C. The changes in microbial population, pH and titratable acidity as well as sugar and ‎organic acid metabolism during the fermentation period were evaluated‎. Appropriate growth of L. plantarum and L.delbruckii resulted in an increase in acidity to 1.86 and 1.75, and a decline in pH to 3.4 and 3.56, respectively after 72 hours. Glucose and fructose were significantly consumed by the strains. Citric acid concentration reduced to 1.12 g/l and 5.8 g/l in the extract fermented by L. plantarum and L. delbrueckii, respectively after 72 hours. Lactic acid was produced by L. plantarum and L. Delbrueckii with 23.8 and 11.4 g/l lactic acid, respectively at the end of fermentation. L.plantarum cells exhibited more resistance toward cold storage conditions. So that at the end of the third week of cold storage conditions, the viable cells of L. plantarum was 3.2 × 101 × CFU / mL, while no viable cells of L.delbruekii was observed at this week. In general, it can be concluded that the fermentation of jujube by L. plantarum and L. delbrueckii could be considered as an appropriate way to improve the functional properties of beverages.
 

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Today, use of functional food, such as probiotic products, is important due to their health benefits against diseases. In this study, the essential oil of Ducrosia anethifolia and Teucrium polium (DAEO and TPEO, respectively) were added to probiotic yogurt (Lactobacillus acidophilus and Bifidobacterium bifidum bacteria) to investigate their effects on the survival of probiotic bacteria during 21 days and assess its various properties. The results showed upon increasing the concentration of DAEO and TPEO, the value of acidity and viscosity of yogurt treatments increased, and the pH and syneresis showed a decreasing trend (P<0.05). The survivability of the investigated probiotic bacteria demonstrated a decreased trend during storage in all treatments, but finally, the number of probiotic bacteria in all treatments was significantly higher than that of the control samples. Based on the results of all tests, the addition of 0.03% of DAEO is the best way to realize the goals of the research.

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Dark chocolate as a best-selling product worldwide with the main health-oriented ingredients such as cocoa powder and butter, along with a high level of consumer awareness of maintaining and enhancing health, reinforces the idea of ​​enriching all types of chocolate with probiotic strains. The aim of this study was to investigate the effects of the presence of microencapsulated bacteria Lactobacillus acidophilus, Streptococcus thermophilus and Bifidobacterium using sodium alginate and resistant corn starch on physicochemical properties including moisture, acidity, water activity, particle size distribution and texture, the plastic and apparent viscosity of probiotic dark chocolate were investigated during storage for 180 days at 4 ° C and 25 ° C to determine the storage temperature. In order to analyze the data obtained from the research, factorial experiment in the form of a completely randomized design was used and the comparison of means was performed by Duncanchr('39')s multiple range test at the probability level (α = 1%). According to the results, in days 0, 7, 30, 90, 150 and 180 physicochemical, chocolate and rheological properties at two storage temperatures and also between different treatments of probiotic dark chocolate obtained from the strains, no significant difference (P <0.05) was observed. Thus, all three products as probiotic dark chocolate have suitable characteristics in terms of the mentioned characteristics to be present in the consumer market.

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In the first step of this study, Anethum graveolens and Nasturtium officinale essential oils prepared with Clevenger apparatus and then, essential oils ingredients evaluated by GC-Mass. Minimum Inhibitory (MIC) and Minimum Bactericidal Concentration (MBC) and antioxidant activity of essential oils were evaluated at concentrations of 350, 400, 500, 600 and 650 mg/L during 60 days. Then, encapsulated essential oils of A. graveolens and N. officinale in the mentioned concentrations by maltodextrin and coacervation methods to evaluate morphology and particle size distribution. Finally, to achieve the optimal concentration of essential oils in order to further preserve the probiotic population of Bifidobacterium lactis, the viability of this bacterium in doogh was evaluated for 21 days. In the second step of the study, the effect of adding essential oils on optimal concentration to the product investigated according to dry matter content, pH, acidity, serum separation rate, rheology and sensory evaluation. The results showed the essential oils have high terpene and phenolic content, which caused proper antioxidant and antimicrobial activity. The viscosity, consistency and acidity of the final product increased on the other hand, pH and serum separation rate decreased significantly. Considering the results of sensory evaluation and lack of any negative effects at optilam concentrations of 400 mg/L and 500 mg/L for A. graveolens and N. officinale essential oils, respectively, the possibility of its industrial application can be examined.

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Lukom is a sort of food that is mainly composed of sugar and starch and, it creates variety in the consumer basket as a snack. Sugar is used as a sweetener in the production of lukom. Grape juice contains high amounts of natural sugars, minerals, vitamins, organic acids and antioxidants, so grape juice can be used as a sweetener as a sugar alternative. In this study, different levels for starch, sugar and grape juice were determined using Design Expert software. For this purpose, 16 treatments were selected using the software for independent variables, including 10-15% starch, 7-35% sugar and 1.5 - 35% was grape juice. The tests included pH, water activity, stiffness, hardness and adhesion force of the tissue and sensory evaluation. Also, after selecting the optimal sample, the survival rate of probiotic bacteria in a fourteen-day period was assessed. The results showed that a linear model was proposed for the trend of all the studied factors so that only the amount of grape juice and sugar was significant for pH. Grape juice and starch also had a greater effect on water activity. With changes in the percentage of starch, sugar and grape juice, the light intensity decreased and the changes in grape juice were more noticeable. The adhesion force and texture stiffness for the independent variables showed the fit between the selected model and the increase in grape juice, stiffness and adhesion of the texture of Lukom samples had a significant increase in the study area. The number of probiotics consumed during the 14-day period was significantly reduced. In the sensory evaluation of the samples, a significant difference was observed between the produced samples and the overall score was calculated to be about 4.1.

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The aim of this study was to investigate the effect of different ratios of starter and kind of probiotic culture on the physicochemical, textural, microbial and sensory properties of probiotic soy cheese. Different ratios of starter (50% thermophilic + 50% mesophilic , 25% thermophilic + 75% mesophilic, 75% thermophilic + 25% mesophilic) with different species of probiotic bacteria (Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium lactis were used to produce probiotic soy cheese. The results were analyzed in a completely randomized design consisting of 12 treatments with 3 replications using two-way analysis of variance. The means were compared by Duncan's multiple range test at 5% probability level. The results showed that treatment 10 (cheese sample containing starter compound (75% thermophilic + 25% mesophilic) + Lactobacillus acidophilus probiotic bacterium) had the lowest pH and the highest acidity. The highest amount of acetic acid was related to treatment 12 (cheese sample containing starter compound (75% thermophilic + 25% mesophilic) + Bifidobacterium lactis probiotic bacterium). In all samples of probiotic soy cheese, the population of probiotic bacteria was in the range of 7-8 log cfu / g. Treatment 6 (cheese sample containing starter compound (25% thermophilic + 75% mesophilic) + Lactobacillus acidophilus probiotic bacterium) had the highest hardness, cohesiveness and tissue springiness, also treatment 6 had the highest sensory quality score, therefore this sample the best treatment was selected. Overall, the results showed that probiotic soy cheese is a suitable environment for the growth of probiotic bacteria and the production of a functional product.
 

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Due to the importance of diet in consumer health, the use of functional foods has become widespread. Foods containing probiotics are a good example of these foods, which have both high nutritional value and prevent disease. In addition, the use of bioactive compounds in food formulations is also technologically important. Natural protection with the use of biological preservatives, in addition to improving food safety and increasing its shelf life, as a technological effect, also has a positive effect on promoting consumer health. The mechanisms by which probiotic bacteria develop health-promoting properties can be complex and multifaceted. These beneficial microorganisms produce a potential and almost unlimited source of bioactive substances that can directly or indirectly affect human health. In this regard, active and live cultures of probiotic bacteria can be used to produce postbiotic bioactive compounds, such as exopolysaccharides, enzymes, organic acids, bioactive and antimicrobial peptides such as bacteriocins and unsaturated fatty acids such as conjugated linoleic acid. In recent years, new concepts related to probiotics, such as postbiotics, have been used to describe inanimate microorganisms or extracts free of bacteria that may provide benefits to the host by providing additional bioactivity to probiotics. Therefore, the present review article investigates various aspects of postbiotics and their bioactivity with the aim of expressing the valuable and practical potential of these compounds for the food industry.

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The aim of this study was to identify the lactic acid of the dominant bacteria in pickled garlic, using initial biochemical tests and PCR technique and to investigate their potential probiotic potential. For this purpose, to determine the probiotic potential of these isolates, some confirmatory tests including resistance to acid, resistance to simulated gastric conditions, resistance to bile salts, activity against pathogenic bacteria, susceptibility to some common antibiotics and hemolytic activity of isolates were performed. Based on BLAST results, five isolates of Lactobacillus plantarum, two isolates as Pediococcus ethanolidurans and one isolate as Lactobacillus brevis were identified. The lactic acid bacteria isolated in this study had the ability to survive at pH 2.5, grow in medium containing 0.3% bile salt, were sensitive to antibiotics and lacked hemolytic activity and therefore have probiotic properties. Supernatant of isolates of this study in high concentrations had the ability to inhibit growth of pathogenic bacteria and the lethal activity against them. Finally, the isolates of this study can be added to other products in the future as a strain with probiotic potential.

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Paraprobiotic is a new group of functional compounds that obtains from the inactivation of a certain probiotics strain. In the present study, the effect of using paraprobiotic Lactobacillus gasseri and storage time (30 days) and temperature (4 and 24 ˚C) on the physicochemical and sensory properties of grape juice was investigated. Grape drink characteristics such as pH, acidity, total soluble solids, turbidity, and sensory attributes were evaluated. The addition of paraprobiotics did not change pH and acidity, and the control specimen (pure grape juice) and the paraprobiotic sample (grape juice containing paraprobiotics) did not differ in this respect. The addition of paraprobiotics increased the turbidity and total soluble solid in the paraprobiotic drink, and these two characteristics also increased with the increase in the storage time.  Considering the sensory properties, the paraprobiotic drink was similar to pure grape juice and had similar overall acceptance scores to pure grape juice, which did not change during storage at different temperatures.
 

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In this study, the survival of Lactobacillus casei probiotic bacteria added to polylactic acid-nanocellulose nanocomposite film during 16 days of storage in the refrigerator was investigated. For this purpose, three edible films including polylactic acid, polylactic acid + nano cellulose, polylactic acid + nano cellulose+ Lactobacillus casei (9 log CFU/g) were prepared and the physical and mechanical characteristics of the films as well as the survival of Lactobacillus casei bacteria were investigated. The results of the mechanical test showed that the use of probiotic bacteria decreased the tensile strength and elongation at break of the polylactic acid-nanocellulose film (P < 0.05), but the addition of nanocellulose improved the mechanical properties of the polylactic acid film. The results of physical tests including humidity, solubility, water vapor permeability showed that the addition of probiotic bacteria and nanocellulose improved the physical properties of the film, but the opacity of the films increased (P<0.05). According to the results of the present study, with the increase in storage time, the survival of probiotic bacteria in the nanofilm decreased, and at the end of the storage period, its values were equal to 6.12 log CFU/g, but it was within the permissible range (6 log CFU/g).Therefore, the composition of Lactobacillus casei probiotic strain in the edible film can be its suitable carrier at refrigerator temperature