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Showing 2 results for Scopolamine
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Volume 2, Issue 1 (9-2011)
Abstract
Atropine and scopolamine are two important tropane alkaloids, widely use as anticholinergic drugs. Such as most solanaceae plants, Atropa belladonna produces these two tropane alkaloids. Carbohydrates are not only act as carbon sources for solanaceus plants in vitro cultures, but also have some effects as signal molecules on tropane alkaloids metabolism. Effect of sorbitol, mannitol and sucrose on growth, chlorophyll, hyoscyamine and scopolamine contents, and hyoscyamine 6- beta hydroxylase (H6H) gene expression, investigated. These treatments decreased the growth of plantlets but increased chlorophyll, hyoscyamine and scopolamine contents. The treatments increase h6h gene expression in different organs of Atropa belladonna plantlets. There was an agreement between h6h expression pattern and scopolamine contents.
P. Fathi Rezaei, E. Rakee,
Volume 10, Issue 3 (9-2019)
Abstract
Datura (Datura stramonium) is known as a rich source of tropane alkaloids, including scopolamine and hyoscyamine as parasympatholytics that competitively antagonize acetylcholine. Production of secondary metabolites often occurs in plants against the various elicitors or signal molecules. In this study, the effect of yeast extract as a biotic elicitor on tropane alkaloid production was evaluated. Surface-sterilized Datura seeds were cultured on 1/2 MS medium supplemented with different concentrations of yeast extract (0, 1.5, and 3g/L). One month after germination, alkaloid yield of Datura plantlets was measured by the use of High-Performance Liquid Chromatography (HPLC). Also, total protein content and antioxidative enzymes activity were determined by spectrophotometry method. According to the results, the fresh weight of root and shoot parts of Datura plantlets at 1.5g/L yeast extract was increased about 2 and 4 times, respectively. Yeast extract (1.5g/L) caused to 1.7 times increase of hyoscyamine amount of root and shoot parts and 2.5 folds scopolamine of Datura plantlets. Moreover, on yeast extract (1.5g/L)-treated plantlets, total protein content, and activity of catalase and guaiacol peroxidase were almost the same as the control group. Altogether, yeast extract (1.5g/L) can be used as a good candidate for enhancement production of tropane alkaloids especially hyoscyamine and scopolamine with high medicinal value.
