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Showing 2 results for Sperm Parameters
Matinsadat Ghafelebashi, Parvaneh Maghami, Abdolhossin Shahverdi, Davoud Doranian, Marjan Sabbaghian,
Volume 11, Issue 3 (10-2020)
Abstract
According to the formation and evolution of life along with static magnetic fields,the permanent exposure has given adaptive ability to beings. Therapeutic magnetism is one of the branches of complementary medicine which uses the low intensity and non-harmful magnetic fields to the body. By studying in infertile couples (20% male factor), the only cause of infertility and in 50% of cases it is considered as an intermediate factor. One of the influential factors in infertility in men is sperm. In the present study, normal specimens in the magnetic field under the intensities of 1,6 and 12 millitesla and at 1,3 and 5 h intervals.
Sperm movement rate was evaluated by CASA, as well as sperm viability, by eosin staining of necrosin and morphology by staining Papanicula. The results of this step on normal sperm showed a significant reduction in the sperm movement ,which that was not affected by the field.
Morphological studies also show that sperm motility is not affected by magnetic field.. the survival rate of sperm was affected by the magnetic field was significantly reduced, and the sperm morphology remained unchanged
Volume 16, Issue 1 (8-2013)
Abstract
Objective: Antioxidants are essential for sperm motility. Calligonum extract possesses the important antioxidants catechin and quercetin. This study investigates the effects of calligonum extract on sperm parameters and the rate of apoptosis in testes of aging male mice. Methods: We initially performed a dose response test with using three doses of calligonum (10 mg/kg, 30 mg/kg and 50 mg/kg). A total of 25 aging male mice (11-13 months) were divided into the following groups of five mice each: control, sham and three experimental groups. The experimental groups received IP injections of calligonum extract (10 mg/kg, 30 mg/kg, 50 mg/kg) weekly for up to five weeks. The sham group received IP injections of DMSO. At the end of the injection period, mice were sacrificed and sperm parameters analyzed. To determine apoptosis in testes, we performed TUNEL staining. Results: Our results showed that after calligonum treatment, there were improved sperm parameters in the 30 mg/kg-treated group compared to the other groups (P≤0.05). Conclusion: Calligonum extract (30 mg/kg) can improve sperm parameters and decrease apoptosis in the testes of aging male mice. This herbal extract can be employed as an antioxidant component for clinical usage.