Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

---

Salmonella is the serious and prevalent bacteria that has important role in epidemic infections. Beside increase of antibiotics resistance in bacteria make that abundance researches did for introduction of replacement method for beard with bacteria infections. Copper NANO oxide particles are components that their anti-microbial nature be evidenced. In this research for discover of NANO particles probably mechanism on the genome of bacteria, salmonella selected as a model for Gram-negative bacteria. In this regard, at first the bacteria were treated with 30 and 60 µg/ml copper oxide NANO particles. At time intervals of 2, 4, and 24 hours. In this doses, bacteria was growth .So bacteria were treated with 90 and 120 µg/ml copper oxide NANO particles. In these doses growth of bacteria even after 24 h completely were stopped .Then their DNA were extracted. In order to investigate the effects of copper oxide NANO particles on the genome, the chain reaction techniques of (RAPD-PCR) was employed .Using the software NTSYS-PC, the results obtained from electrophoresis of PCR products on agarose gel were analysis. The results of the study revealed that copper oxide NANO particles not only affects the growth of bacteria but also affect the sequencing of genomic DNA and leads to the changes of them in different points.

Keywords: salmonella", " RAPD polymerase chain reaction (RAPD-PCR), " copper nano oxide particles "

Full-Text [PDF 739 kb]   (4812 Downloads)    

Add your comments about this article : Your username or Email:

---