Volume 9, Issue 3 (2018)
JMBS 2018, 9(3): 435-440 |
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Amirinezhad M, Yousefzadi M, Arman M, Rahimzadeh M. Comparison of Essential Oils Toxicity of Satureja Khuzistanica and Satureja Rechingeri on Larvae of the Barnacle Amphibalanus Amphitrite. JMBS 2018; 9 (3) :435-440
URL: http://biot.modares.ac.ir/article-22-13450-en.html
URL: http://biot.modares.ac.ir/article-22-13450-en.html
1- Marine Biology Department, Marine Science & Technology Faculty, Hormozgan University, Bandar Abbas, Iran
2- Marine Biology Department, Marine Science & Technology Faculty, Hormozgan University, Bandar Abbas, Iran, University of Hormozgan, Minab Road, Bandar Abbas, Hormozgan Province, Iran , morteza110110@gmail.com
3- Biology Department, Pyam-e-Noor University, Tehran, Iran
4- Biochemistry Department, Medicine Faculty, Hormozgan University of Medical Science, Bandar Abbas, Iran
2- Marine Biology Department, Marine Science & Technology Faculty, Hormozgan University, Bandar Abbas, Iran, University of Hormozgan, Minab Road, Bandar Abbas, Hormozgan Province, Iran , morteza110110@gmail.com
3- Biology Department, Pyam-e-Noor University, Tehran, Iran
4- Biochemistry Department, Medicine Faculty, Hormozgan University of Medical Science, Bandar Abbas, Iran
Abstract: (3433 Views)
Aims: Barnacles are benthos crustacean with a calcareous place. In the state of puberty, they do not move and stick on their feet to the objects in the water. The life cycle of a typical barnacle includes two stages. The aim of this study was to compare essential oils toxicity of Satureja khuzistanica and Satureja rechingeri on larvae of the barnacle Amphibalanus amphitrite.
Materials and Methods: In this experimental study, leaves of S. khuzestanica and S. rechingeri were collected. The extraction lasted 3 to 4 hours. Essential oil composition was detected by Gas chromatography–mass spectrometry (GC-MS). In order to evaluate the toxicity, the effect of essential oils with 50, 25, 12.5, 6.25, 3.125, and 1.5µg/ml concentrations was investigated on larvae of the barnacle Amphibalanus amphitrite. For data analysis, one way ANOVA, SPSS 16 software, Probit analysis with 95% confidence interval, and Excel 2010 were used.
Findings: Both S. khuzestanica and S. rechingeri had a high toxicity effect on larvae of the barnacle Amphibalanus amphitrite, which had a 100% lethal effect at 50μg/ml concentration and with increasing concentrations, more mortality was observed in the barnacle larval stages. S. khuzestanica with LC50 of 23.48μg/ml had a stronger effect on stage II nauplius. Stages 5 and 6 of barnacle larvae were also more susceptible than the rest of the stages.
Conclusion: Both S. rechingeri and S. khuzestanica have a high toxicity effect on larvae of the barnacle Amphibalanus amphitrite.
Materials and Methods: In this experimental study, leaves of S. khuzestanica and S. rechingeri were collected. The extraction lasted 3 to 4 hours. Essential oil composition was detected by Gas chromatography–mass spectrometry (GC-MS). In order to evaluate the toxicity, the effect of essential oils with 50, 25, 12.5, 6.25, 3.125, and 1.5µg/ml concentrations was investigated on larvae of the barnacle Amphibalanus amphitrite. For data analysis, one way ANOVA, SPSS 16 software, Probit analysis with 95% confidence interval, and Excel 2010 were used.
Findings: Both S. khuzestanica and S. rechingeri had a high toxicity effect on larvae of the barnacle Amphibalanus amphitrite, which had a 100% lethal effect at 50μg/ml concentration and with increasing concentrations, more mortality was observed in the barnacle larval stages. S. khuzestanica with LC50 of 23.48μg/ml had a stronger effect on stage II nauplius. Stages 5 and 6 of barnacle larvae were also more susceptible than the rest of the stages.
Conclusion: Both S. rechingeri and S. khuzestanica have a high toxicity effect on larvae of the barnacle Amphibalanus amphitrite.
Article Type: _ |
Subject:
Agricultural Biotechnology
Received: 2016/05/4 | Accepted: 2017/01/24 | Published: 2018/09/22
Received: 2016/05/4 | Accepted: 2017/01/24 | Published: 2018/09/22
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