Volume 9, Issue 2 (2018)
JMBS 2018, 9(2): 219-226 |
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Seyedalipour B, Dadoei Z, Ebrahimzadeh M. Antioxidant Activity and Free Radical Scavenging of the Extract Obtained from Alchemilla persica by Percolation, Polyphenol Fraction, and Ultrasonic Methods. JMBS 2018; 9 (2) :219-226
URL: http://biot.modares.ac.ir/article-22-24445-en.html
URL: http://biot.modares.ac.ir/article-22-24445-en.html
1- Cellular & Molecular Biology Department, Basic Sciences Faculty, University of Mazandaran, Babolsar, Iran, Cellular & Molecular Biology Department, Basic Sciences Faculty, University of Mazandaran Pardis, Babolsar, Iran. Postal code: 4741695447 , b.alipour81@gmail.com
2- Biochemistry Department, Biology Faculty, Science & Research Branch, Islamic Azad University, Sanandaj, Iran
3- “Pharmaceutical Sciences Research Center” and “Medicinal Chemistry Department, Pharmacy Faculty”, Mazandaran University of Medical Sciences, Sari, Iran
2- Biochemistry Department, Biology Faculty, Science & Research Branch, Islamic Azad University, Sanandaj, Iran
3- “Pharmaceutical Sciences Research Center” and “Medicinal Chemistry Department, Pharmacy Faculty”, Mazandaran University of Medical Sciences, Sari, Iran
Abstract: (5168 Views)
Aims: Alchemilla L. genus (Rosaceae) has various biological activities such as anti-inflammatory, anticarcinogenic, antioxidant and antimicrobial. The aim of the present study was to investigate antioxidant activity and free radical scavenging of the extract obtained from Alchemilla persica by percolation, polyphenol fraction, and ultrasonic methods.
Materials and Methods: In the present experimental research, Alchemilla persica was used and percolation, polyphenol, and ultrasonics methods were used for extraction and the antioxidant activities of the extracts were determined by different tests, including 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging activity, Iron chelating activity, and reducing power. Total phenolic and flavonoid content were measured by the Folin Ciocalteu and AlCl3 methods, respectively. The data were analyzed by SPSS 22 software, using one-way ANOVA and tukey test.
Findings: In DPPH radical scavenging activity, the polyphenol extract had a significant different with other extracts (p=0.001). Polyphenolic extract showed higher reducing power than other extacts and Vitamin C (p=0.001). Percolation extract had higher amounts of total phenolic and flavonoid content than other extracts.
Conclusion: Polyphenolic extracts have the highest DPPH, nitric oxide scavenging activity, Iron chelating activity, and reducing power compared to ultrasonic and percolation methods. Aerial parts of Alchemilla persica extracts have high levels of antioxidant activity including phenols and flavonoids.
Materials and Methods: In the present experimental research, Alchemilla persica was used and percolation, polyphenol, and ultrasonics methods were used for extraction and the antioxidant activities of the extracts were determined by different tests, including 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging activity, Iron chelating activity, and reducing power. Total phenolic and flavonoid content were measured by the Folin Ciocalteu and AlCl3 methods, respectively. The data were analyzed by SPSS 22 software, using one-way ANOVA and tukey test.
Findings: In DPPH radical scavenging activity, the polyphenol extract had a significant different with other extracts (p=0.001). Polyphenolic extract showed higher reducing power than other extacts and Vitamin C (p=0.001). Percolation extract had higher amounts of total phenolic and flavonoid content than other extracts.
Conclusion: Polyphenolic extracts have the highest DPPH, nitric oxide scavenging activity, Iron chelating activity, and reducing power compared to ultrasonic and percolation methods. Aerial parts of Alchemilla persica extracts have high levels of antioxidant activity including phenols and flavonoids.
Subject:
Agricultural Biotechnology
Received: 2016/09/4 | Accepted: 2017/12/6 | Published: 1937/03/31
Received: 2016/09/4 | Accepted: 2017/12/6 | Published: 1937/03/31
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