Volume 4, Issue 2 (2013)                   JMBS 2013, 4(2): 1-8 | Back to browse issues page

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Talesh Sasani S, Mohammad Soltani B, Behmanesh M, Safaie N. Detection of Avr-pita1 gene in Rhizoctonia solani AG1-IA. JMBS 2013; 4 (2) :1-8
URL: http://biot.modares.ac.ir/article-22-5290-en.html
Detection of Avr-pita1 gene in Rhizoctonia solani AG1-IA
Soheila Talesh Sasani1 , Bahram Mohammad Soltani * 2, Mehrdad Behmanesh3 , Naser Safaie3
1- PhD. student / Dept. Genetics, Fac. Biol. Sci., TMU, Tehran
2- Assisstant professor/TMU, Tehran
3- Associate professor/TMU, Tehran
Abstract:   (9922 Views)
Sheath blight, caused by Rhizoctonia solani AG1-IA, is one of the most destructive disease. Conventional methods of disease control using fungicides may develop new problems. Therefore, understanding molecular mechanisms of plant–pathogen interaction is necessary to adopt effective approaches for managing the disease. Here for the first time, by using bioinformatics tools and RT-PCR analysis and sequencing confirmed the presence of a Magnaporthe oryzae Avr-pita gene orthologous sequence designated as Rhiz-pita1 gene in three different geographic isolates of R. solani AG1- IA( A2,R1 and T2) genome. SignalP program predicted a secretion signal upstream of Rhiz-pita1 gene. Nucleotide sequences of 5' region of Rhiz-pita1 gene from geographical isolates showed 99% identity in exons and 100% in introns which are characteristics of fast evolving effector proteins. Also, 98% homology between Rhiz-pita and M.oryza-pita1gene suggests that Rhiz-pita encodes an effector protein. Howevere, more researchs are necessary to confirm of this suggestion. Keywords: Rhizoctonia solani, signal peptide Rice blast , Effector
Keywords: Rhizoctonia solani, Rhizoctonia solani, signal peptide Rice blast, Effector
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Article Type: _ | Subject: Biotechnology
Received: 2014/08/4 | Accepted: 2013/09/23 | Published: 2015/02/1
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