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Optimization of Fermentative Production of Cephalosporin C by Acromonium chrysogenum Strain using Response Surface Method
Omid Seyedvand1 , Ghazal Labbeiki * 2, Saeid Akbarzadeh kolahi3
1- MsC of Microbial Biotechnology , Department of Biotechnology ,Faculty of Advanced Sciences and Technology , Tehran Medical Sciences , Islamic Azad University, Tehran , Iran
2- Assistant Professor, Department of Food Sciences and Technology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences , Islamic Azad University, Tehran , Iran , labeiki@iaups.ac.ir
3- Assistant Professor, Department of Pharmacology & Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran , Iran
Abstract:   (178 Views)
  The use of productive microorganisms in the production of primary and secondary metabolites is part of the knowledge of microbial biotechnology, which is carried out with the aim of using huge cellular factories and considering their inherent and latent ability to produce products. Investigating and optimizing the factors affecting the synthesis of metabolites will increase the growth of the strain and the yield of the product. In this regard, in this research, by using the response surface method (RSM) and Box-Behnken design, the fermentation production of cephalosporin C by Acromonium chrysogenum PTCC 5271, and the effect of three factors on the fermentation process, including pH of Fermentation culture media, rice bran and magnetite iron oxide nanoparticles have been measured at three levels. The obtained results showed that changes in the concentration of magnetite iron oxide nanoparticles and rice bran in the fermentation media significantly affect the amount of antibiotics produced. The highest amount of Cephalosporin C produced (224 mg/l) was obtained in the culture containing 0.04 mg/l magnetite iron oxide nanoparticles, 2.5 g/l rice bran and pH of 6.5. The optimized values ​​for the factors were calculated as 0.0325 mg/l, 2.6162 g/l, and 6.4545 respectively by the Mini Tab program, and finally the mathematical model for the response variable was obtained. Based on the results of this research, magnetite iron oxide nanoparticles and rice bran are suitable substrates in fermentation culture media and biological processes.
Keywords: Fermentation, Acromonium chrysogenum strain, Cephalosporin C, Response Surface Method
     
Article Type: Original Research | Subject: Microbial biotechnology
Received: 2022/12/23 | Accepted: 2023/06/25
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