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Abstract The amount of waste and agricultural wastes in Iran is very high due to the combination of resources that they have become suitable for ethanol production. Molasses is one of the most abundant and inexpensive carbon sources available and usable for ethanol production. With this application to prevent it from getting into the environment and the product is obtained as a clean and environmental fuel. The main objective of this study was to compare the production of ethanol from molasses by Saccharomyces cerevisiae and Zaymomonas Mobilis. In this study, the fermented juices of grapes are cultivated on the RM culture containing 1% Nystatin at Aerobic conditions and temperature is 30℃. Then the Zymomonas Mobilis was isolated and identified by using staining techniques, biochemical tests, growth in the presence of 7% ethanol and ribotyping. To determine the amount of ethanol production, 10% molasses medium was used. The amount of ethanol at 24, 48, 96, 120, 144 hours in 10% molasses were 1.45, 3.4 and 5.05% for Zymomonas mobili subsp. mobilis IRMH52, Zymomonas mobilis subsp. mobilis ATCC 10988 and Saccharomyces cerevisiae, respectively. In this study, a new strain of Zymomonas mobilis were isolated and compared the production of ethanol with the same conditions showed that this strain of Saccharomyces cerevisiae to produce less ethanol.

Keywords: Ethanol, Zymomonas mobilis, Saccharomyces cerevisiae, molasses

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