Volume 8, Issue 2 (2017)
JMBS 2017, 8(2): 15-24 |
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Hassanshahian M. Study the crude oil degrading bacteria associated with bivalve Crassostrea gigas collected from Persian Gulf (Bandar Abbas provenance). JMBS 2017; 8 (2) :15-24
URL: http://biot.modares.ac.ir/article-22-4646-en.html
URL: http://biot.modares.ac.ir/article-22-4646-en.html
1- Kerman
Abstract: (9946 Views)
Crude oil is comprised of four groups: Saturates Aromatics, Resins and Asphaltenes. Oil pollution has irreversible effects on marine ecosystems. Therefore paying attention to oil pollution and the management of the ports is very important. Biodegradation of oil derivatives is more effective, more powerful and more economically efficient method for remediation in polluted sites rather than physicochemical methods. In this study, for isolation of crude oil degrading bacteria, seawater and mussels were collected from Persian Gulf. Enumeration of bacteria were done in collected samples. Isolated bacteria were identified by biochemical and molecular tests. Crude oil biodegradation for each strain was assessed by spectrophotometer and Gas Chromatography (GC). The results of this study show that the quantity and biodiversity of heterotrophic and crude oil degrading bacteria in Crassostrea gigas mussels was higher than surrounding environment (seawater). Eleven crude oil degrading bacteria were isolated from Persian Gulf, 7 strains were identified biochemically and 2 strains were selected on the basis of higher degradation. These isolated strains were identified as Shewanella and Alcanivorax. The half percentage of oil was removed by these strains in 15 days of incubation. These bacteria could be used for cleanup oil-polluted marine areas after more research and field observation.
Article Type: Research Paper |
Subject:
Agricultural Biotechnology
Received: 2015/12/15 | Accepted: 2017/09/23 | Published: 2018/01/27
Received: 2015/12/15 | Accepted: 2017/09/23 | Published: 2018/01/27
References
1. Speight, J.G. (1999) The chemistry and technology of petroleum.Marcel DekkerNew York, 3rd Edition.
2. Todd, P.A., Ong, X., and Chou, L.M. (2010) Impacts of pollution on marine life in Southeast Asia. Biodivers. Conserv.19(4), 1063-1082.
3. Hanson, K.G., Anuranjini, N., Madhavi, K., and Anjana, J.D. (1997) Bioremediation of crude oil contamination with Acinetobacter.sp A3. Curr. Microbiol. 35(3), 191-193
4. Hasanshahian, M., and Emtiazi, G. (2008) Investigation of alkane biodegradation using the microtiter plate method and correlation between biofilm formation, biosurfactant production and crude oil biodegradation. Int. Biodegrad. 62, 170-178
5. Hassanshahian, M., Emtiazi, G., and Cappello, S. (2012) Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar. Pollut. Bull.64, 7-12.
6. Cavallo, R.A., Acquaviva, M.I., and Stabili, L. (2009) Culturableheterotrophic bacteria in sea water and Mytilus galloprovincialisfrom a Mediterranean area (Northern Ionian Sea –Italy). Environ. Monitor. Assess.149, 465–475.
7. Subarna, R., Dipak, H., Debabrata, B., Dipa, B., and Ranajit, K. (2002) Survey of petroleum-degrading bacteria in coastalwaters of Sunderban Biosphere Reserve. World J Microb Biot.18, 575–581.
8. Balba, M.T., Al-Awadhi, N., and Al-Daher, R. (1998) Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluation. J. Microbiol. Methods.32(2), 155–164
9. Wrenn, B.A., and Venosa, A.D. (1996) Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most probable number procedure. J Microbiol.42(3), 252–258.
10. Fuchsluger, C., Preims, M., and Fritz, I. (2010) Automated measurement and quantification of heterotrophic bacteria in water samples based on the MPN method. J. Ind. Microbiol. Biotechnol.38, 241-247
11. Holt, S.G., Kriey, N.R., Sneath, P.H.A., Staley, J.T and Williams, S.T. (1998) Bergy’s Manual of Determinative for Bacteriology. Williams and Wilkins, New York.
12. Yakimov, M.M., Timmis, K.N and Golyshin, P.N. (2007). Obligate oil-degrading marine bacteria. Curre. Opin. Biotechnol. 18(3), 257-266.
13. Rahman, K.S.M., Thahira-Rahman, J., Lakshmanaperumalsamy, P., and Banat, I.M. 2004. Towards efficient crude oil degradation by a mixed bacterial consortium. Bioresour Technol. 85, 257-261.
14. Wang, L., Tang, Y., Wang, Sh., Liu, R., Liu, M.Z., Zhang, Y., Liang, F.L and Feng, L. (2006) Isolation and characterization of a novel thermophilic Bacillus straindegrading long-chain n-alkanes. Extremophiles. 10, 347–356.
15. Distel, D.L. (1998) Evolution of chemoautotrophic endo symbiosis in bivalves: Bivalve-bacteria chemosymbiosis are phylogenetically diverse but morphologically similar. Biol. Sci.48, 277-286.
16. Al-Awadhi, H., Sdashti, N. (2012) waters of the Arabian Gulf. Int. Biodeterior. Biodeg.69, 10–16.
17. Gutierrez, T.,Singleton, D., Berry, D.,Yang, T.,D.Aitken, M.,andTeske, A. (2013) Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP. ISME J. 7, 2091-2104.
18. Prieur, D., Mevel, G., Nicolas, J.L., Plusguellec, A and Vigneulle, M. (1990) Interactions between bivalve mollusks and bacteria in the marine environment. Ocean. Mar. Biol. Ann. Rev. 28, 277-352
19. Cappello, S., Russo, D., Santisi, S., and Calogero, R. (2011) Presence of hydrocarbon-degrading bacteria in the gills of mussel Mytilusgallo provincialis in a contaminated environment: a mesoscale simulation study. Chem. Ecol. 3, 1–14.
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