Volume 6, Issue 1 (2015)                   JMBS 2015, 6(1): 40-50 | Back to browse issues page

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Antioxidant properties of mucus and oral plate crude extract of Stichodactyla haddoni. JMBS 2015; 6 (1) :40-50
URL: http://biot.modares.ac.ir/article-22-10326-en.html
Abstract:   (9268 Views)
A high population of the sea anemone inhabit in the intertidal zone of the Hormoz Island. The tidal zones generally are characterized by severe environment stresses (vis, UV and high temperature), that can increase the intracellular oxidation and free radicals as well. Oxidation and free radicals is paved by the animal defensive mechanisms, including accumulation of antioxidant compounds and enzymes. In this study, carpet anemones sampled from intertidal zone of the eastern city of Hormoz Island (IRAN). Extraction was performed by using of PBS and methanol 40% solvents: 2 g dry weight of the mucous, by 2 replications, and 10 g wet weight of the oral plate, by 6 replications, from 6 samples. Different concentrations of extracts were made for antioxidant tests. The antioxidant properties of extract were measured by DPPH and FRC methods. Results showed that a correlation is between the antioxidant activity and the concentration of extracts in two methods. In DPPH assay, IC50 values was 1.469±0.208, 1.85±0.016 for mucus extract by PBS and methanol 40%, respectively, and it was 0.733±0.06, 0.444±0.036 mg/ml, for oral plate extract by PBS and methanol 40%. Our results showed that the antioxidant activity of oral plate was significantly higher than mucous in this anemone.
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Article Type: Research Paper | Subject: Biotechnology
Received: 2015/01/5 | Accepted: 2015/09/23 | Published: 2015/10/13

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