Volume 8, Issue 2 (2017)                   JMBS 2017, 8(2): 160-170 | Back to browse issues page

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Zarinkamar F, tajik S. Evaluation of antioxidant activity and phenolic content from saffron organs (Crocus sativus L.). JMBS. 2017; 8 (2) :160-170
URL: http://biot.modares.ac.ir/article-22-119-en.html
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Abstract:   (5922 Views)
in recent years, interest in use of plant sources due to function of Phytochemicals and antioxidant compound in maintenance of human health is increased, phenolic and flavonoid compounds are one of important compounds of plants that have antioxidant effects. Aim of the present study is to examine and comparison of phenolic and flavonoid compounds and antioxidant capacity of different organs of saffron, that every year a huge amount of them are wested during the processing of stigma. In this study, different parts of saffron were extracted by methanol (80%), then the amount of total phenol and flavonoid compounds was assayed by means of Folin-Ciocalteu and and Aluminium choloride methods respectively. The antioxidant activity was measured by DPPH free radical reduction. According to the results of this study, The highest content of total phenolic(6.43 mg GAE g-1 DW) and flavonoid (1.33 mg RU g-1 DW) was observed in stigma compared to other organs. The result of DPPH test also showed higher antioxidant capacity of stigma in comparison to other organs. Comparison of phenolic compounds in various organs showed that the content of these compounds and antioxidant activity could be different related to type of organs. Also, the higher antioxidant capacity in stigma and tepal compared to leaf and corm could be as a result of more phenolic compounds in these organs.
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Article Type: Research Paper | Subject: Agricultural Biotechnology
Received: 2016/03/6 | Accepted: 2017/09/23 | Published: 2018/01/27

1. Shrififar, F., Moshafi, M.H.,Mansouri, S.H. (2007). In vitro evaluation of antibacterial and antioxidant of the essential oil and methanol extract of endemic Zatariamultiflora Boiss. Food Control 18: 800-805.
2. Naczk,M., Amarowicz,R., Zadernowski,R., Pegg, R., Shahidi,F. (2003). Antioxidant activity of crude phenolic extracts from wild blueberry leaves. Vol. 12/53, SI 1, pp. 166–169.
3. Sanchez-Vioquea,b.R., Rodreguez-Condea M., Reina-Ure ̃naa J., Escolano-Terceroa M., Herraiz-Pe ̃nalvera D.Santana-Méridasa,b(2012). In vitro antioxidant and metal chelating properties of corm, tepal and leaf from saffron (Crocus sativusL.). ndustrial Crops and Products 39,149–153
4. Ghasemzadeh A., Jaafar H., Karimi E.,Ibrahim M.(2012).Combined effect of CO2 enrichment and foliar application of salicylic acid on the production and antioxidant activities of anthocyanin, flavonoids and isoflavonoids from ginger. BMC Complementary and Alternative Medicine, 12, 1-12.
5. Wojdylo, A., Oszmianski, J., Czemerys, R.,(2007).Antioxidant activity and phenolic compounds in 32 selected herbs, FoodChem., 105: 940–949.
6. Goli S. A.H, Mokhtari F & Rahimmalek M (2012).Phenolic Compounds and Antioxidant Activity from Saffron (Crocus sativusL.) Petal. Journal of Agricultural Science; 4, 175-181.
7. Abdullaev FI. Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativusL.). Exp Biol Med. 2002; 227: 20-25
8. Gil, M.I., Tomás-Barberán, F.A.,Hess-Pierce, B., and Kader, A.A. (2002). Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. Journal of Agricultural and Food Chemistry 50: 4976-4982.
9. Li, C.Y., Lee, E.J., Wu, T.S., (2004). Antityrosinase principles and constituents of the petals of Crocus sativus. J. Nat. Prod. 67, 437–440.
10. Hosseinzadeh, H., Younesi, H.M., (2002). Antinocicetive and anti-inflammatory effects of Crocus sativusL. stigma and petals extracts in mice. BMC Pharmacol. 2, 1–8
11. Fernández, J.A., (2004). Biology, biotechnology and biomedicine of saffron. Recent Res. Dev. Plant Sci. 2, 127–159
12. Esmaeili, N., Ebrahimzadeh, H., Abdi, K., Safarian, S., (2011). Determination of some phenolic compounds in Crocus sativusL. corms and its antioxidant activities study. Phcog. Mag. 7, 74–80
13. Niknam,V. Ebrahimzadeh, H.(2002).Phenolics content in Astragalus species. Pak. J. Bot., 34 (3): 283-289.
14. Pinelo, M., Rubilar,M., Sineiro, J., & Nunez, M. J (2004).Extraction of antioxidant phenolics from almond hulls (Prunus amygdalus) and pine sawdust (Pinus pinaster). Food Chemistry, 85, 267-273.
15. Chang C., Yang M., Wen H., Chern J.(2002).Estimation of total flavonoid content in Propolis by two complementary calorimetric methods. Journal of Food and Drug Analysis; 10: 178-18.
16. Singh, R. P., Murthy, K. N. C., & Jayaprakasha, G. K.(2002).Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of Agricultural and Food Chemistry, 50, 81-86.
17. Braca, N.D. Tommasi, L.D. Bari, C. Pizza, M. Politi, I. Morelli (2001). Antioxidant principles from Bauhinia terapotensis. J.Nat. Prod., 64, 892–895
18. Gursoy, N., Sarikurkcu, C., cengiz, M., & Solak, M. H. (2009). Antioxidant activities, metal contents, total phenolics and flavonoids of seven morchell species. Food and Chemical Toxicology, 47, 2381-2388.
19. Ahmad B.S., Malik A.H., Wani Z.A., Mohiuddin T., Shah Z., Abbas N., Ashraf N. (2015). Phytochemical analysis and antioxidant activity of different tissue types of Crocus sativusand oxidative stress alleviating potential of saffron extract in plants, bacteria, and yeast. South African Journal of Botany 99 , 80–87.
20. Falleh,H., Ksouri,R., Medini,F., Guyot, S., Abdelly,C., Magné,C. (2011). Antioxidant activity and phenolic composition of the medicinal and edible halophyte Mesembryanthemum edule L. Industrial Crops and Products 34.
21. Carbone, K., Giannini B., Picchi V., Lo Scalzo R., Cecchini F.(2011).Phenolic composition and free radical scavenging activity of different apple varieties in relation to the cultivar, tissue type and storage. Food Chemistry. 127, 493–500.
22. Carvalho IS, Cavaco T., Brodelius M.(2011).Phenolic composition and antioxidant capacity of six Artemisia species. IND Crop Prod.33, 382–388
23. Trabelsi, N., Falleh H., Jallali I., Daly A., Hajlaoui H., Smaoui A, Abdelly C., Ksouri R (2012). Variation of phenolic composition and biological activities in Limoniastrum monopetalum L organs. Acta Physiol Plant.34:87–96
24. Falleh, H., Ksouri, R., Chaieb, K., Karray-Bouraoui, N., Trabelsi, N., Boulaaba, M., Abdelly, C.(2008).Phenolic composition of Cynara cardunculusL. organs, and their biological activities, C. R. Biologies. 331 372–379.
25. Lisiewska, Z., Kmiecik, W., Korus, A.(2006) .Content of vitamin C, carotenoids, chlorophylls and polyphenols in green parts of dill (Anethum graveolens L.) depending on plant height, Journal of Food Composition and Analysis. 19 134–140.
26. Gardner, P.T., White, T.A.C., McPhail, D.B., and Duthie, G.C. (2000).The relative contributions of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chemistry 68: 471-474
27. Conforti, F., Statti, G. A. and Menichini, F. (2007).Chemical and biological variability of hot pepper fruits (Capsicum annuum var. acuminatum L.) in relation to maturitystage. Food Chemistry 102: 1096–1104.
28. Ksouri, R., Falleh, H., Megdiche, W., Trabelsi, N., Hamdi, B., Chaieb, K., Bakhrouf, A.,Magné, C., Abdelly, C.(2009).Antioxidant and antimicrobial activities of the ediblemedicinal halophyte Tamarix gallicaL. and related polyphenolic constituents.Food Chem. Toxicol. 47, 2083–2091
29. Matkowski, A., Zielin ́ ska, S., Oszmian ́ ski, J., Lamer-Zarawska, E (2008).Antioxidantactivity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. Biores. Technol. 99, 7892–7896
30. Shan, B.; Cai, Y.Z.; Sun, M.; Corke, H(2005).Antioxidant capacity of 26 spice extracts andcharacterization of their phenolic constituents. J. Agric. Food Chem., 53, 7749-7759.
31. Karimi, E., Oskoueian, E., Hendra, R., & Jaafar, H. Z. E (2010).Evaluation of Crocus sativusL. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules, 15, 6244-6256
32. Akhondzadeh BA, Ghoreishi SA. Noorbala AA, Akhondzadeh SH, Rezazadeh SH (2008).Petal and stigma of Crocus sativusL. in the treatment of depression: A pilot double-blind randomized trial. J Med Plants.; 7: 29-36
33. Chen Y., Zhang H., Tian X., Zhao C., Cai L., Liu Y., Jia L., Yin HX., Chen C.(2008).Antioxidant potential of crocins and ethanol extracts of Gardenia jasminoidesELLIS andCrocus sativusL. A relationship investigation between antioxidant activity and crocin contents. Food Chem. 109: 484-492.
34. Tajik S., Zarinkamar F., Niknam V.(2015).Effects of Salicylic Acid on Carotenoids and Antioxidant Activity of Saffron (Crocus sativusL.).Applied food biotechnology. 2: 33-37.
35. Mashmoul M., Azlan A.,Khazaai Mohd Yusof B.N., Mohd N.S.(2013).Saffron: a natural potent antioxidant as a promising anti-obesity drug. Antioxidant. 2: 293-308
36. Assimopoulou, A., Sinakos,Z., Papageorgiou, P.(2005). Radical Scavenging Activity of Crocus sativus L. Extract and its Bioactive Constituents. Phytother. Res. 19, 997–1000.

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