Volume 9, Issue 4 (2018)
JMBS 2018, 9(4): 621-626 |
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Zafari J, Javani Jouni F, Satari keykaleh M, Abdolmaleki P, Khodayar M, Jalali A. Toxicity of Cisplatin under the Influence of Static Magnetic Field in Susceptible and Drug-Resistant Cells. JMBS 2018; 9 (4) :621-626
URL: http://biot.modares.ac.ir/article-22-13684-en.html
URL: http://biot.modares.ac.ir/article-22-13684-en.html
J. Zafari1 
, F. Javani Jouni2 , M. Satari keykaleh3 , P. Abdolmaleki4 , M.J. Khodayar5 , A. Jalali * 6
, F. Javani Jouni2 , M. Satari keykaleh3 , P. Abdolmaleki4 , M.J. Khodayar5 , A. Jalali * 6
1- “Toxicology Research Center” and “Toxicology Department, Pharmacy Faculty”, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Microbiology Department, Biological Sciences Faculty, Tehran North Branch, Islamic Azad University, Tehran, Iran
3- Biology Department, Sciences Faculty, Malayer University, Hamedan, Iran
4- Biophysics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
5- Toxicology Department, Pharmacy Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
6- “Toxicology Research Center” and “Toxicology Department, Pharmacy Faculty”, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Pharmacy Faculty, Ahvaz Jundishapur University of Medical Sciences, Golestan Boulevard, Ahvaz, Iran. Postal Code: 6135733184 ,amjalali@hotmail.com
2- Microbiology Department, Biological Sciences Faculty, Tehran North Branch, Islamic Azad University, Tehran, Iran
3- Biology Department, Sciences Faculty, Malayer University, Hamedan, Iran
4- Biophysics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
5- Toxicology Department, Pharmacy Faculty, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
6- “Toxicology Research Center” and “Toxicology Department, Pharmacy Faculty”, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Pharmacy Faculty, Ahvaz Jundishapur University of Medical Sciences, Golestan Boulevard, Ahvaz, Iran. Postal Code: 6135733184 ,
Abstract: (5278 Views)
Aims: Regarding the treatment of cancer, due to the limitation in the use of high dose and resistance of cancer cells, it is necessary to use optimal methods that have high therapeutic efficacy and reduce the dose of radiation and medicine. The aim of the present research was to investigate toxicity of cisplatin under the influence of static magnetic field in susceptible and drug-resistant cell.
Materials and Methods: In the present experimental study, A2780-CP resistant cell classes and susceptible to A2780 cisplatin were investigated in the field and drug-treated cell groups compared to the drug-receiving group alone, and to determine the effect of static magnetic field and concentration of drug, 10mT for 24 hours and logarithmic drug concentration (1, 10, 50, 100, and 500mcg/ml) were used. Inhibitory concentration of 50% cell growth (IC50) was obtained for the cells in the absence and presence of the magnetic field after conversion of the absorption obtained in the ELISA from the MTT test to cytotoxicity percentage. Data were analyzed with Prism software using two-way ANOVA and T-test.
Findings: In the presence of a static magnetic field and different drug concentrations, a greater reduction in the percentage of In vivo cells was observed. IC50 values for A2780 cells in the absence and presence of magnetic fields were 27.69±9.58 and 8.96±1.48μg/ml for A2780-CP, and 61.61±8.03 and 9.58±3.13μg/ml, respectively.
Conclusion: The mortality rate of the cells treated with cisplatin under the influence of the magnetic field is more in susceptible and drug-resistant cells than that of only drug use. Drug-resistance decreases in the drug-resistant cell class in the presence of a magnetic field.
Materials and Methods: In the present experimental study, A2780-CP resistant cell classes and susceptible to A2780 cisplatin were investigated in the field and drug-treated cell groups compared to the drug-receiving group alone, and to determine the effect of static magnetic field and concentration of drug, 10mT for 24 hours and logarithmic drug concentration (1, 10, 50, 100, and 500mcg/ml) were used. Inhibitory concentration of 50% cell growth (IC50) was obtained for the cells in the absence and presence of the magnetic field after conversion of the absorption obtained in the ELISA from the MTT test to cytotoxicity percentage. Data were analyzed with Prism software using two-way ANOVA and T-test.
Findings: In the presence of a static magnetic field and different drug concentrations, a greater reduction in the percentage of In vivo cells was observed. IC50 values for A2780 cells in the absence and presence of magnetic fields were 27.69±9.58 and 8.96±1.48μg/ml for A2780-CP, and 61.61±8.03 and 9.58±3.13μg/ml, respectively.
Conclusion: The mortality rate of the cells treated with cisplatin under the influence of the magnetic field is more in susceptible and drug-resistant cells than that of only drug use. Drug-resistance decreases in the drug-resistant cell class in the presence of a magnetic field.
Article Type: Research Paper |
Subject:
Agricultural Biotechnology
Received: 2016/09/21 | Accepted: 2018/02/9 | Published: 2018/12/21
Received: 2016/09/21 | Accepted: 2018/02/9 | Published: 2018/12/21
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