Volume 9, Issue 2 (2018)
JMBS 2018, 9(2): 227-232 |
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Khorasani A, Firoozabadi S, Shankayi Z. Conductivity Changes of Liver Tissue during Irreversible Electroporation and Calculation of the Electric Field Distribution. JMBS 2018; 9 (2) :227-232
URL: http://biot.modares.ac.ir/article-22-14227-en.html
URL: http://biot.modares.ac.ir/article-22-14227-en.html
1- Medical Physics Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Medical Physics Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Medical Physics Department, Medical Sciences Faculty, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran ,pourmir@modares.ac.ir
2- Medical Physics Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Medical Physics Department, Medical Sciences Faculty, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran ,
Abstract: (5893 Views)
Aims: In irreversible electroporation process, the membrane of cancer cells is damaged irreversibly by electric pulses of high-intensity field, which in turn leads to cell death. Factors influencing the field distribution include voltage, pulse width, and electric conductivity of tissue. The present study was conducted with the aim of evaluating conductivity changes of liver tissue during irreversible electroporation and calculation of the electric field distribution.
Materials and Methods: In the present experimental study, using simulation, the relationship between pulse width and voltage intensity of each pulse was investigated in conductivity changes during irreversible electroporation, and the electric field distribution was calculated. In this simulation, in order to solve the equations, the software COMSOL 5 was used. Needle electrodes were used, and the liver tissue was considered as the target tissue. Eight pulses with the stimulated frequency of 1Hz, pulse width of 100µs and 2ms, and the intensity of the electric fields ranging from 1000 to 3000v/cm were used as electric pulses.
Findings: Conductivity of tissue increased during sending the electrical pulses. The conductivity changes in the tip of the electrodes were more than the area between the two rows of electrodes. As the intensity of the pulsed electric field increased, the tissue conductivity also increased. When the conductivity of the tissue was constant and variable, the maximum electric field intensity was obtained 3879 and 3448v/cm.
Conclusion: While electric pulse transmission, tissue conductivity increases. The electric field distribution depends on the conductivity at the desired point and by changing this conductivity due to the electroporation, the electric field distribution also changes and the maximum intensity of the electric field decreases.
Materials and Methods: In the present experimental study, using simulation, the relationship between pulse width and voltage intensity of each pulse was investigated in conductivity changes during irreversible electroporation, and the electric field distribution was calculated. In this simulation, in order to solve the equations, the software COMSOL 5 was used. Needle electrodes were used, and the liver tissue was considered as the target tissue. Eight pulses with the stimulated frequency of 1Hz, pulse width of 100µs and 2ms, and the intensity of the electric fields ranging from 1000 to 3000v/cm were used as electric pulses.
Findings: Conductivity of tissue increased during sending the electrical pulses. The conductivity changes in the tip of the electrodes were more than the area between the two rows of electrodes. As the intensity of the pulsed electric field increased, the tissue conductivity also increased. When the conductivity of the tissue was constant and variable, the maximum electric field intensity was obtained 3879 and 3448v/cm.
Conclusion: While electric pulse transmission, tissue conductivity increases. The electric field distribution depends on the conductivity at the desired point and by changing this conductivity due to the electroporation, the electric field distribution also changes and the maximum intensity of the electric field decreases.
Subject:
Agricultural Biotechnology
Received: 2016/10/6 | Accepted: 2018/02/11 | Published: 2018/06/21
Received: 2016/10/6 | Accepted: 2018/02/11 | Published: 2018/06/21
References
1. Dunki-Jacobs EM, Philips P, Martin RC. Evaluation of resistance as a measure of successful tumor ablation during irreversible electroporation of the pancreas. J Am Coll Surg. 2014;218(2):179-87. [Link] [DOI:10.1016/j.jamcollsurg.2013.10.013]
2. Shankayi Z, Firoozabadi SM, Hassan ZS. Optimization of electric pulse amplitude and frequency in vitro for low voltage and high frequency electrochemotherapy. J Membr Biol. 2014;247(2):147-54. [Link] [DOI:10.1007/s00232-013-9617-9]
3. Shankayi Z, Firoozanadi SM, Saraf Hassan Z. Comparison of low voltage amplitude electrochemotherapy with 1 Hz and 5 KHz frequency in volume reduction of mouse mammary tumor in Balb/c Mice. Koomesh. 2012;13(4):486-90. [Persian] [Link]
4. Shankayi Z, Pourmirjafari Firoozabadi SM, Zohair Saraf H. The endothelial permeability increased by low voltage and high frequency electroporation. J Biomed Phys Eng. 2013;3(3):87-92. [Link]
5. Čorović S, Pavlin M, Miklavčič D. Analytical and numerical quantification and comparison of the local electric field in the tissue for different electrode configurations. Biomed Eng Online. 2007;6:37. [Link] [DOI:10.1186/1475-925X-6-37]
6. Lu DS, Kee ST, Lee EW. Irreversible electroporation: Ready for prime time?. Tech Vasc Interv Radiol. 2013;16(4):277-86. [Link] [DOI:10.1053/j.tvir.2013.08.010]
7. Garcia PA, Davalos RV, Miklavcic D. A numerical investigation of the electric and thermal cell kill distributions in electroporation-based therapies in tissue. PLoS One. 2014;9(8):e103083. [Link] [DOI:10.1371/journal.pone.0103083]
8. Adeyanju OO, Al-Angari HM, Sahakian AV. The optimization of needle electrode number and placement for irreversible electroporation of hepatocellular carcinoma. Radiol Oncol. 2012;46(2):126-35. [Link] [DOI:10.2478/v10019-012-0026-y]
9. Corovic S, Zupanic A, Miklavcic D. Numerical modeling and optimization of electric field distribution in subcutaneous tumor treated with electrochemotherapy using needle electrodes. IEEE Trans Plasma Sci. 2008;36(4):1665-72. [Link] [DOI:10.1109/TPS.2008.2000996]
10. Moisescu MG, Radu M, Kovacs E, Mir LM, Savopol T. Changes of cell electrical parameters induced by electroporation, a dielectrophoresis study. Biochim Biophys Acta. 2013;1828(2):365-72. [Link] [DOI:10.1016/j.bbamem.2012.08.030]
11. Kranjc M, Bajd F, Serša I, Miklavčič D. Magnetic resonance electrical impedance tomography for measuring electrical conductivity during electroporation. Physiol Meas. 2014;35(6):985-96. [Link] [DOI:10.1088/0967-3334/35/6/985]
12. Ivorra A, Rubinsky B. In vivo electrical impedance measurements during and after electroporation of rat liver. Bioelectrochemistry. 2007;70(2):287-95. [Link] [DOI:10.1016/j.bioelechem.2006.10.005]
13. Corovic S, Lackovic I, Sustaric P, Sustar T, Rodic T, Miklavcic D. Modeling of electric field distribution in tissues during electroporation. Biomed Eng Online. 2013;12:16. [Link] [DOI:10.1186/1475-925X-12-16]
14. Sano MB, Neal II RE, Garcia PA, Gerber D, Robertson J, Davalos RV. Towards the creation of decellularized organ constructs using irreversible electroporation and active mechanical perfusion. Biomed Eng Online. 2010;9:83. [Link] [DOI:10.1186/1475-925X-9-83]
15. Pliquett UF, Schoenbach KH. Changes in electrical impedance of biological matter due to the application of ultrashort high voltage pulses. IEEE Trans Dielectr Electr Insul. 2009;16(5):1273-9. [Link] [DOI:10.1109/TDEI.2009.5293938]
16. Lackovic I, Magjarevic R, Miklavcic D. Three-dimensional finite-element analysis of joule heating in electrochemotherapy and in vivo gene electrotransfer. IEEE Trans Dielectr Electr Insul. 2009;16(5):1338-47. [Link] [DOI:10.1109/TDEI.2009.5293947]
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