Volume 9, Issue 1 (2018)                   JMBS 2018, 9(1): 9-16 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghoreishi F, Etemadifar ‎ Z. Heavy Metal Removal by Phosphate Solubilizing Extraced from ‎Metal Waste and Phosphatase Enzyme Role in the Removal ‎. JMBS 2018; 9 (1) :9-16
URL: http://biot.modares.ac.ir/article-22-14277-en.html
1- Biology Department, Science Faculty, University of Isfahan, Isfahan, Iran
2- Biology Department, Science Faculty, University of Isfahan, Isfahan, Iran, Biology Department, Science Faculty, University of Isfahan, Hezar Jarib Sreet, Isfahan, Iran. Postal Code: ‎‎8174673441‎ , zetemedifar@gmail.com
Abstract:   (10032 Views)
Aims: Heavy metals are one of the most important pollutants in earth and water environments due to long-term durability. The aim of this study was to isolate phosphate solubilizing bacteria from metal waste, investigate the amount of resistance, remove the metal by it and the effect of phosphatase on removal of metals.
Materials & Methods: In this experimental study, the isolation of phosphate solubilizing bacteria and detection of isolates were carried out, using biochemical and molecular tests. The phosphatase was measured by colorimetric method, the resistance of the separated to the metals with the minimum inhibitory concentration (MIC50), minimum bactericidal concentration (MBC) and the rate of removal of metals by atomic absorption was measured. The surface changes of the exposed metal cells were investigated by Fourier Transform Infrared Spectroscopy (FTIR) and the effect of phosphatase on metal removal. Data analysis was done with Duncan's test, using Excel 2013 and SPSS 20 software.
Findings: Serratia proteamaculans was identified as producer of the acid phosphatase. The highest MIC and MBC were obtained for Nickel (Ni) and Lead (Pb), respectively. The most metal removal was for Pb. MIC50 of Chrome and Cadmium were obtained less than 0.1mM and 1mM, and their removal percentage by the isolate were 18% and 48%, respectively. According to the FTIR, 988.339cm-1 wavelength was observed in the cells treated by 5mM Pb that is related to the Pb3(PO4)2. The isolate showed the highest resistance and removal of Pb. The mechanism of Ni removal was associated to the cell surface, while Pb was removed by both of the cells and supernatant containing phosphatase.
Conclusion: Serratia proteamaculans is the phosphate solubilizing bacterium in metal waste. This bacterium produces an enzyme called phosphatase, which is a cause of lead removal.
Full-Text [PDF 642 kb]   (3464 Downloads)    
Article Type: Research Paper | Subject: Agricultural Biotechnology
Received: 2016/04/19 | Accepted: 2017/10/7 | Published: 2018/05/22

1. Martinez RJ, Beazley MJ, Sobecky PA. Phosphate-mediated remediation of metals and radionuclides. Adv ‎Ecol. 2014;2014:786929.‎ [Link]
2. Godt J, Scheidig F, Grosse-Siestrup C, Esche V, Brandenburg P, Reich A, et al. The toxicity of cadmium and ‎resulting hazards for human health. J Occup Med Toxicol. 2006;1:22.‎ [Link]
3. Järup L. Hazards of heavy metal contamination. Br Med Bull. 2003;68:167-82.‎ [Link] [DOI:10.1093/bmb/ldg032]
4. Shen HM, Zhang QF. Risk assessment of nickel carcinogenicity and occupational lung cancer. Environ ‎Health Perspect. 1994;102(Suppl 1):275-82.‎ [Link] [DOI:10.1289/ehp.94102s1275]
5. Pellerin C, Booker SM. Reflections on hexavalent chromium: Health hazards of an industrial heavyweight. ‎Environ Health Perspect. 2000;108(9):A402-7.‎ [Link] [DOI:10.1289/ehp.108-a402]
6. Zaidi A, Saghir Khan M, Ahemad M, Oves M, Wani PA. Recent advances in plant growth promotion by ‎phosphate-solubilizing microbes. In: Saghir Khan M, Zaidi A, Musarrat J, editors. Microbial strategies for crop ‎improvement. Berlin: Springer Science & Business Media; 2009. pp. 23-50.‎ [Link] [DOI:10.1007/978-3-642-01979-1]
7. Lovley DR, Phillips EJP. Bioremediation of uranium contamination with enzymatic uranium reduction. ‎Environ Sci Technol. 1992;26(11):2228-34.‎ [Link] [DOI:10.1021/es00035a023]
8. Martinez RJ, Beazley MJ, Taillefert M, Arakaki AK, Skolnick J, Sobecky PA. Aerobic uranium (VI) ‎bioprecipitation by metal‐resistant bacteria isolated from radionuclide-and metal-contaminated subsurface ‎soils. Environ Microbiol. 2007;9(12):3122-33.‎ [Link] [DOI:10.1111/j.1462-2920.2007.01422.x]
9. Appukuttan D, Rao AS, Kumar Apte Sh. Engineering of deinococcus radiodurans R1 for bioprecipitation of ‎uranium from dilute nuclear waste. Appl Environ Microbiol. 2006;72(12):7873-8.‎ [Link] [DOI:10.1128/AEM.01362-06]
10. Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA. Phosphate solubilizing microbes: Sustainable approach for ‎managing phosphorus deficiency in agricultural soils. Springerplus. 2013;2:587.‎ [Link] [DOI:10.1186/2193-1801-2-587]
11. Nautial CS. An efficient microbiological growth medium for screening phosphate solubilizing ‎microorganisms. FEMS Microbiol Lett. 1999;170(1):265-70.‎ [Link] [DOI:10.1111/j.1574-6968.1999.tb13383.x]
12. Cowan ST, Steel KJ. Cowan and Steel's manual for the identification of medical bacteria. Barrow GI, ‎Feltham RKA, editors. Cambridge: Cambridge university press; 2004.‎ [Link]
13. Chen Wp, Kuo TT. A simple and rapid method for the preparation of gram-negative bacterial genomic ‎DNA. Nucleic Acids Res. 1993;21(9):2260.‎ [Link] [DOI:10.1093/nar/21.9.2260]
14. Kier LD, Weppelman R, Ames BN. Resolution and purification of three periplasmic phosphatases of ‎Salmonella typhimurium. J Bacteriol. 1977;130(1):399-410.‎ [Link]
15. Araggon V, Kurtz S, Cianciotto NP. Legionella pneumophila major acid phosphatase and its role in ‎intracellular infection. Infect Immun. 2001;69(1):177-85.‎ [Link] [DOI:10.1128/IAI.69.1.177-185.2001]
16. Robinson JW. Atomic absorption spectroscopy. Anal Chem. 1960;32(8):17A-29.‎ [Link] [DOI:10.1021/ac60164a712]
17. Schmitt J, Flemming HC. FTIR-spectroscopy in microbial and material analysis. Int Biodeter Biodegr. ‎‎1998;41(1):1-11.‎ [Link] [DOI:10.1016/S0964-8305(98)80002-4]
18. Miller FA, Wilkins CH. Infrared spectra and characteristic frequencies of inorganic ions. Anal Chem. ‎‎1952;24(8):1253-94.‎ [Link] [DOI:10.1021/ac60068a007]
19. Rameshkumar P, Pothana Sh, Manivannan G, Murali M. Heavy metal response behaviors of sulfur-‎oxidizing Pseudomonas sp. PRK786. Int J Adv Biotechnol Res. 2014;15(2):106-16.‎ [Link]
20. Zwaig N, Milstein C. The amino acid sequence around the reactive serine residue in alkaline phosphatase ‎of serratia marcescens. Biochem J. 1964;92(2):421-2.‎ [Link] [DOI:10.1042/bj0920421]
21. Tahri Joutey N, Bahafid W, Sayel H, Ananou S, El Ghachtouli N. Hexavalent chromium removal by a ‎novel Serratia proteamaculans isolated from the bank of Sebou River (Morocco). Environ Sci ‎Pollut Res. 2014;21(4):3060-72.‎ [Link] [DOI:10.1007/s11356-013-2249-x]
22. Pflicke H. Purification of Serratia sp. phosphatase, identification/localisation of the two phosphatase ‎isoenzymes and large scale production of the enzyme [Intrnet]. Munich: Grin Verlag; 2003 [cited 2017 May ‎‎10]. Available from: https://www.grin.com/document/18268.‎ [Link]
23. Rajendran P, Muthukrishnan J, Gunasekaran P. Microbes in heavy metal remediation. Indian J Exp Biol. ‎‎2003;41(9):935-44.‎ [Link]
24. Javanbakht V, Alavi SA, Zilouei H. Mechanisms of heavy metal removal using microorganisms as ‎biosorbent. Water Sci Technol. 2014;69(9):1775-87.‎ [Link] [DOI:10.2166/wst.2013.718]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.