Volume 11, Issue 1 (2020)
JMBS 2020, 11(1): 21-28 |
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Karbalaei-Heidari H, Taghavi L, Hasani Zadeh P. Induction of Biosurfactant Production from a Native Isolated Moderately Halophilic Bacterium, Halomonas sp. MM93 in the Presence of Olive Oil and Study of its Stability. JMBS 2020; 11 (1) :21-28
URL: http://biot.modares.ac.ir/article-22-15105-en.html
URL: http://biot.modares.ac.ir/article-22-15105-en.html
1- Biology Department, Sciences Faculty, Shiraz University, Shiraz, Iran, Biology Department, Sciences Faculty, Shiraz University, Shiraz, Iran , karbalaei@shirazu.ac.ir
2- Biology Department, Sciences Faculty, Shiraz University, Shiraz, Iran
3- Biological Sciences Faculty, University of Tehran, Tehran, Iran
2- Biology Department, Sciences Faculty, Shiraz University, Shiraz, Iran
3- Biological Sciences Faculty, University of Tehran, Tehran, Iran
Abstract: (4672 Views)
Biosurfactants are valuable microbial metabolites that have considerable applications in different industries. They offer so many advantages over their synthetic counterparts such as biodegradability, low toxicity, activity at extreme conditions, ability to be produced from renewable wastes and by-products. In the present study, biosurfactant production of Halomonas sp. MM93 in nutrient broth medium at 30°C after 72h was investigated using oil spreading and hemolysis tests. The emulsification capacity of the biosurfactant was also evaluated in a defined production medium during 96h. Effect of olive oil, n-Hexan, and kerosene as hydrophobic carbon sources to induction of biosurfactant production by the strain MM93 was also investigated. Due to the importance of stability in the case of industrial use, the effect of extreme temperature, pH and salinity on the stability of bacterial culture supernatant was evaluated. This strain created a clear zone of 2.5cm diameter in an oil-spreading test and its E24 index was 45%. Halomonas sp. MM93 could reduce the surface tension of the culture medium from 70 to 40 mN/m. Also, the produced biosurfactant showed remarkable stability at high temperature (100°C), extreme acidic and alkaline conditions (pH=2-12), and high salinity (20g/L). According to obtained data, native isolated moderately halophilic bacterium, Halomonas sp. MM93 could be considered as a potent strain in terms of producing stable biosurfactants for various industries especially the processes of increasing microbial recovery of oil that need Compounds with High surface activity and high stability.
Article Type: Original Research |
Subject:
Microbial biotechnology
Received: 2019/10/28 | Accepted: 2019/09/4 | Published: 2020/03/18
Received: 2019/10/28 | Accepted: 2019/09/4 | Published: 2020/03/18
References
1. Fakruddin MD. Biosurfactant: Production and application. J Pet Environ Biotechnol. 2012;3(4):1000124. [Link]
2. Mulligan CN, Yong RN, Gibbs BF. On the use of biosurfactants for the removal of heavy metals from oil‐contaminated soil. Environ Prog. 1999;18(1):50-4. [Link] [DOI:10.1002/ep.670180120]
3. Reis R, Pacheco G, Pereira A, Freire D. Biosurfactants: Production and applications. New York: InTech; 2013. [Link] [DOI:10.5772/56144]
4. Bezza FA, Nkhalambayausi Chirwa EM. Biosurfactant-enhanced bioremediation of aged polycyclic aromatic hydrocarbons (PAHs) in creosote contaminated soil. Chemosphere. 2016;144:635-44. [Link] [DOI:10.1016/j.chemosphere.2015.08.027]
5. Bustamante M, Duran N, Diez MC. Biosurfactants are useful tools for the bioremediation of contaminated soil: A review. J Soil Sci Plant Nutr. 2012;12(4):667-87. [Link] [DOI:10.4067/S0718-95162012005000024]
6. Al-Sulaimani H, Joshi S, Al-Wahaibi Y, Al-Bahry S, Elshafie A, Al-Bemani A. Microbial biotechnology for enhancing oil recovery: Current developments and future prospects. Biotechnol Bioinf Bioeng. 2011;1(2):147-58. [Link]
7. Muthusamy K, Gopalakrishnan S, Ravi TK, Sivachidambaram P. Biosurfactants: Properties, commercial production and application. Curr Sci. 2008;94(6):736-47. [Link]
8. Banat IM, Samarah N, Murad M, Horne R, Banerjee S. Biosurfactant production and use in oil tank clean-up. World J Microbiol Biotechnol. 1991;7(1):80-8. [Link] [DOI:10.1007/BF02310921]
9. Abdollahi M, Karbalaei Heidari HR. Isolation, identification, biochemical properties and potential application of an organic solvent-tolerant lipase from Pseudomonas sp. strain NEB-1. Iran J Sci Technol. 2014;38(3):221-9. [Link]
10. Mulligan CN, Cooper DG, Neufeld RJ. Selection of microbes producing biosurfactants in media without hydrocarbons. J Ferment Technol. 1984;62(4):311-4. [Link]
11. Qazi MA, Kanwal T, Jadoon M, Ahmed S, Fatima N. Isolation and characterization of a biosurfactant‐producing Fusarium sp. BS‐8 from oil contaminated soil. Biotechnol Prog. 2014;30(5):1065-75. [Link] [DOI:10.1002/btpr.1933]
12. Cooper DG, Goldenberg BG. Surface-active agents from two Bacillus species. Appl Environ Microbiol. 1987;53(2):224-9. [Link] [DOI:10.1128/AEM.53.2.224-229.1987]
13. Jain DK, Collins-Thompson DL, Lee H, Trevors JT. A drop-collapsing test for screening surfactant-producing microorganisms. J Microbiol Methods. 1991;13(4):271-9. [Link] [DOI:10.1016/0167-7012(91)90064-W]
14. Dhasayan A, Kiran GS, Selvin J. Production and characterisation of glycolipid biosurfactant by Halomonas sp. MB-30 for potential application in enhanced oil recovery. Appl Biochem Biotechnol. 2014;174(7):2571-84. [Link] [DOI:10.1007/s12010-014-1209-3]
15. Kebbouche-Gana S, Gana ML, Khemili S, Fazouane-Naimi F, Bouanane NA, Penninckx M, et al. Isolation and characterization of halophilic Archaea able to produce biosurfactants. J Ind Microbiol Biotechnol. 2009;36(5):727-38. [Link] [DOI:10.1007/s10295-009-0545-8]
16. Youssef NH, Duncan KE, Nagle DP, Savage KN, Knapp RM, McInerney MJ. Comparison of methods to detect biosurfactant production by diverse microorganisms. J Microbiol Methods. 2004;56(3):339-47. [Link] [DOI:10.1016/j.mimet.2003.11.001]
17. Willumsen PA, Karlson U. Screening of bacteria, isolated from PAH-contaminated soils, for production of biosurfactants and bioemulsifiers. Biodegradation. 1996;7(5):415-23. [Link] [DOI:10.1007/BF00056425]
18. Chakrabarty AM. Genetically-manipulated microorganisms and their products in the oil service industries. Trends Biotechnol. 1985;3(2):32-9. [Link] [DOI:10.1016/0167-7799(85)90056-3]
19. Tulloch AP, Spencer JF, Gorin PA. The fermentation of long-chain compounds by torulopsis magnoliae: I. structures of the hydroxy fatty acids obtained by the fermentation of fatty acids and hydrocarbons. Can J Chem. 1962;40(7):1326-38. [Link] [DOI:10.1139/v62-203]
20. Karanth NG, Deo PG, Veenanadig NK. Microbial production of biosurfactants and their importance. Curr Sci. 1999;77(1):116-26. [Link]
21. Bento FM, De Oliveira Camargo FA, Okeke BC, Frankenberger Jr WT. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol Res. 2005;160(3):249-55. [Link] [DOI:10.1016/j.micres.2004.08.005]
22. Priya T, Usharani G. Comparative study for biosurfactant production by using Bacillus subtilis and Pseudomonas aeruginosa. Bot Res Int. 2009;2(4):284-7. [Link]
23. Khopade A, Biao R, Liu X, Mahadik K, Zhang L, Kokare C. Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination. 2012;285:198-204. [Link] [DOI:10.1016/j.desal.2011.10.002]
24. Chandran PR, Das N. Biosurfactant production and diesel oil degradation by yeast species Trichosporon asahii isolated from petroleum hydrocarbon contaminated soil. Int J Eng Sci Technol. 2010;2(12):6942-53. [Link]
25. Obayori OS, Ilori MO, Adebusoye SA, Oyetibo GO, Omotayo AE, Amund OO. Degradation of hydrocarbons and biosurfactant production by Pseudomonas sp. strain LP1. World J Microbiol Biotechnol. 2009;25(9):1615-23. [Link] [DOI:10.1007/s11274-009-0053-z]
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