Volume 10, Issue 3 (2019)                   JMBS 2019, 10(3): 441-446 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rezanejad F, Hosseini A. The effect of growth factors on direct micropropagation of Physalis alkekengi L. (Solanaceae) through buds and stems explants to transfer to the greenhouse and flowering phase. JMBS 2019; 10 (3) :441-446
URL: http://biot.modares.ac.ir/article-22-16223-en.html
1- Biology Department, Science Faculty, Shahid Bahonar University of Kerman, Kerman, Iran, Biology Department, Science Faculty, Shahid Bahonar University of Kerman, Imam Khomeini Highway, Pajoohsh Square, Kerman, Iran , frezanejad@uk.ac.ir
2- Biology Department, Science Faculty, Shahid Bahonar University of Kerman, Kerman, Iran
Abstract:   (3725 Views)
Physalis alkekengi L. is planted in gardens and green spaces because of the beautiful and colorful sepals surrounding the fruit. The species is widely used in traditional medicine and treating a range of diseases. Micropropagation of P. alkekengi was evaluated using the node and internode explants. After sterilization and seed germination, sterile seedlings were transformed to basal MS medium to create sterilized seedlings as a source of explants. Regeneration of nodes and internodes explants was studied at various concentrations of growth regulators of 2, 4-D and BAP as well as in medium lacking growth regulators or control (11 various media). The experiment was conducted in a completely randomized design with three replicates. The internodal explants produced shoot on media 2 (0.2mgl-12, 4-D), 3 (0.2mgl-12, 4-D+0/2mgl-1 BAP ), and 4 (0.5mgl-12, 4-D+0.2mgl-1 BAP ) and then were rooted on these media. The nodal explants in control and different hormonal treatments generated shoots; interestingly, shoots generated in control medium successfully established roots on the same medium after 7 days (70%). The other regenerated shoots in different media (10) were rooted on ½ MS medium containing 1mgl-1IBA. The rooted plants were transplanted into pots containing sand as well as perlite to be well acclimated before transfer to the greenhouse. They grew well later in the greenhouse at a 100% success This study shows high in vitro regeneration capability of this species as an important medicinal and ornamental plant. Therefore, it is suggested to use this species in molecular and genetic studies, somaclonal variation, and the production of herbal medicine.
Full-Text [PDF 878 kb]   (1715 Downloads)    
Article Type: Research Paper | Subject: Agricultural Biotechnology
Received: 2017/09/26 | Accepted: 2018/02/3 | Published: 2019/09/21

1. Get persistent links for your reference list or bibliography. Copy and paste the list, we’ll match with our metadata and return the links. Members may also deposit reference lists here too. 1- Mohager E. New pharmaceutical plant in the North East of Mandragora turcomanica Mizg. J Phytopathol Promot. 2007;1(1):37-41. [Persian] [Link]
2. Bergier K, Kuźniak E, Skłodowska M. Antioxidant potential of Agrobacterium-transformed and non-transformed Physalis ixocarpa plants grown in vitro and ex vitro. Postepy Hig Med Dosw (online). 2012;66:976-82. [Link] [DOI:10.5604/17322693.1023086]
3. Estakhr J, Javdan N. Preliminary study of phytochemical screening and antibacterial activity of Physalis alkekengi against Staphylococcus aureus. Pharmacol online. 2011;3:97-103. [Link]
4. Ahmad W, Khan NA, Ahmad G, Ahmad S. Physico-chemical standardization of seeds of Kaknaj (Physalis alkekengi Linn.). Hamdard Med. 2010;53(3):77-82. [Link]
5. Sharma N, Bano A, Dhaliwal HS, Sharma V. Perspectives and possibilities of Indian species of genus Physalis (L.)- A comprehensive Review. Europ J Pharm Medi Res. 2015;2(2):326-53. [Link]
6. Nasimi M, Heidari Nasrabadi M, Shiravi A. Physalis alkekengi alcoholic fruit extract on the growth of the placenta in pregnant Wistar rats. J Anim Biol. 2009;1(2):51-60. [Persian] [Link]
7. Zarei A, Shariati M, Shekarforoosh S, Changizi Ashtiyani S, Rasekh F. The effect of Physalis alkekengi extract on the physiologic function of organ tissues: a mini-review. J Arak Univ Med Sci. 2012;15(7):94-104. [Persian] [Link]
8. Sunayama R, Kuroyanagi M, Umehara K, Ueno A. Physalin and neophysalin from Physalis alkekengi var. francheti and their differentiation inducing activity. Phytochemistry. 1993;34(2):529-33. [Link] [DOI:10.1016/0031-9422(93)80040-Y]
9. Ramar K, Ayyadurai V, Arulprakash T. In vitro shoot multiplication and plant regeneration of Physalis peruviana L. an important medicinal plant. Int J Curr Microbiol Appl Sci. 2014;3(3):456-64. [Link]
10. Rahula P, Raj VD, Glint K. In vitro plant regeneration in Capsicum chinense Jacq. J Appl Biol Biotechnol. 2015;3(1):030-3. [Link]
11. Jualang-Azlan GI, Marziah M. Production of anti-cancer compound, Physalin B from callus culture of Physalis minima (Linn.). J Adv Res Des. 2015;6(1):21-36. [Link]
12. Purushotam PM, Thottukara-madama A, Duraisamy P, Senthil K. In vitro prppagation and enhancement of phytoconstituent in Withania cougulans, a rare medicinal plant. Bull Environ Pharmacol Life Sci. 2015;4(7):122-31. [Link]
13. Rostami R, Abrishamchi P, lahouti M. The effect different concentrations of 2,4-D on meristem tissue culture of potato (Solanum tuberusum L.). J Sci Shahid Chamran Univ Ahvaz. 2009;1(22):117-22. [Persian] [Link]
14. Shekhawat MS, Kannan N, Manokari M, Ravindran CP. In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures. J Genet Eng Biotechnol. 2015;13(2):209-14. [Link] [DOI:10.1016/j.jgeb.2015.08.002]
15. Bhosale UP, Dubhashi SV, Mali NS, Rathod HP. In vitro shoot multiplication in different species of banana. Asian J Plant Sci Res. 2011;1:23-7. [Link]
16. Padmapriya H, Karthikeyan AVP, Jahir Hussain G, Karthi C, Velayutham P. An efficient protocol for in vitro propagation of Solanum nigrum L. from nodal explants. J Agric Technol. 2011;7(4):1063-73. [Link]
17. Nayak S, Kumar S, Satapathy K, Moharana A, Behera B, Barik D, Acharya L, Mohapatra P, Jena P, Naik S. In vitro plant regeneration from cotyledonary nodes of Withania somnifera (L.) Dunal and assessment of clonal fidelity using RAPD and ISSR markers. Acta Physiol Plant. 2013;35(1):195-203. [Link] [DOI:10.1007/s11738-012-1063-2]
18. Fadel D, Kintzios S, Economou SA, Moschopoulou G, Constantinidou HA. Effect of different strength of medium on organogenesis, phenolic accumulation and antioxidant activity of spearmint (Mentha spicata L.) Open Hortic J. 2010;3(1):31-5. [Link] [DOI:10.2174/1874840601003010031]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.