Volume 10, Issue 1 (2019)                   JMBS 2019, 10(1): 23-27 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimi E, Sadeghi A. Toxicity Effect of Silver Nanoparticles on Two Plant Growth Promoting Streptomyces Spp. Strains, Phytopathogenic Fungi Fusarium Solani and Phytopathogenic Oomycetes Pythium aphanidermatum and Pythium ultimum. JMBS 2019; 10 (1) :23-27
URL: http://biot.modares.ac.ir/article-22-16269-en.html
1- Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran, Agricultural Biotechnology Research Institute of Iran, Shahid Fahmideh Boulevard, Karaj, Iran. Postal Code: 3135933151 , ekarimi@abrii.ac.ir
2- Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Abstract:   (8662 Views)
Silver nanoparticles have antimicrobial activity and are used in various commercially produced products. In this study, the effects of two types of nanosilver formulations, including LS2000 and L2000 on two strains of Streptomyces and three phytopathogenic agents, Pythium aphanidermatum, Pythium ultimum and Fusarium solani were investigated. Streptomyces and phytopathogenic agents were cultured on ISP2 and PDA medium respectively supplemented with 0, 5, 10, 25, 50 and 70ppm of LS2000 and L2000. The influence of LS2000 and L2000 on mycelium of Streptomyces was investigated by atomic force microscopy (AFM). Colony forming unit (cfu) of the bacteria decreased in response to elevated concentrations of L2000. LS2000 completely inhibited growth of both strains at a concentration of 5ppm. The inhibitory effects of LS2000 on the phytopathogenic agents were more than L2000. P. aphanidermatum showed the highest tolerance to L2000 and only at 75ppm of the nanoparticles, the diameter of the colonies was decreased. High susceptibility of F. solani to L2000 caused a decrease in fungal colony diameter in lowest concentration of the nanoparticles. The growth of all phytopathogenic agents was decreased by LS2000 and completely stopped in a concentration of 50ppm. The results showed that LS2000 destroyed mycelial networks of the both bacteria in all tested concentrations. Vesicles appeared on the surface of the mycelium branches, subsequent to treatment with L2000. Based on the results, the inhibitory effects of silver nanoparticles on the beneficial soil bacteria were more than on the phytopathogenic agents. Therefore, more caution should be taken in using silver nanoparticles as a fungicide in agriculture.
Full-Text [PDF 737 kb]   (2425 Downloads)    
Article Type: Research Paper | Subject: Agricultural Biotechnology
Received: 2016/12/7 | Accepted: 2018/02/18 | Published: 2019/03/16

1. Lugtenberg B, Kamilova F. Plant-growth-promoting rhizobacteria. Annu Rev Microbiol. 2009;63:541-56. [Link] [DOI:10.1146/annurev.micro.62.081307.162918]
2. Berg G. Plant-microbe interactions promoting plant growth and health: Perspectives for controlled use of microorganisms in agriculture. Appl Microbiol Biotechnol. 2009;84(1):11-8. [Link] [DOI:10.1007/s00253-009-2092-7]
3. Yandigeri MS, Malviya N, Solanki MK, Shrivastava P, Sivakumar G. Chitinolytic Streptomyces vinaceusdrappus S5MW2 isolated from Chilika lake, India enhances plant growth and biocontrol efficacy through chitin supplementation against Rhizoctonia solani. World J Microbiol Biotechnol. 2015;31(8):1217-25. [Link] [DOI:10.1007/s11274-015-1870-x]
4. Kämpfer P. The family Streptomycetaceae, part I: Taxonomy. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E, editors. The Prokaryotes. New York: Springer; 2006. pp. 538-604. [Link] [DOI:10.1007/0-387-30743-5_22]
5. Koike ST, Gladders P, Paulus AO. Vegetable diseases: A color handbook. Burlington MA: Academic Press; 2007. [Link]
6. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3(1):95-101. [Link] [DOI:10.1016/j.nano.2006.12.001]
7. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004;275(1):177-82. [Link] [DOI:10.1016/j.jcis.2004.02.012]
8. Kim KJ, Sung WS, Suh BK, Moon SK, Choi JS, Kim JG, et al. Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals. 2009;22(2):235-42. [Link] [DOI:10.1007/s10534-008-9159-2]
9. Sadeghi A, Karimi E, Abbaszadeh Dahaji P, Ghorbani Javid M, Dalvand Y, Askari H. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World J Microbiol Biotechnol. 2012;28(4):1503-9. [Link] [DOI:10.1007/s11274-011-0952-7]
10. Karimi E, Sadeghi A, Abbaszadeh Dehaji P, Dalvand Y, Omidvarib M, Kakuei Nezhad M. Biocontrol activity of salt tolerant Streptomyces isolates against phytopathogens causing root rot of sugar beet. Biocontrol Sci Technol. 2012;22(3):333-49. [Link] [DOI:10.1080/09583157.2012.658552]
11. Sadegh Hassani S, Afzali J, Khosravi M. Atomic force microscopy. Tehran: Gisoom; 2014. p. 548. [Persian] [Link]
12. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005;16(10):2346-53. [Link] [DOI:10.1088/0957-4484/16/10/059]
13. Pulit J, Banach M, Szczygłowska R, Bryk M. Nanosilver against fungi: Silver nanoparticles as an effective biocidal factor. Acta Biochim Pol. 2013;60(4):795-8. [Link]
14. Mukha IP, Eremenko AM, Smirnova NP, Mikhienkova AI, Korchak GI, Gorchev VF, et al. Antimicrobial activity of stable silver nanoparticles of a certain size. Appl Biochem Microbiol. 2013;49(2):199-206. [Link] [DOI:10.1134/S0003683813020117]
15. Yang X, Yang W, Wang Q, Li H, Wang K, Yang L, et al. Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia coli and Staphylococcus epidermidis. Talanta. 2010;81(4-5):1508-12. [Link] [DOI:10.1016/j.talanta.2010.02.061]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.