Volume 12, Issue 3 (2021)                   JMBS 2021, 12(3): 123-136 | Back to browse issues page

‎ 20.1001.1.23222115.1400.12.3.1.1

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Mosaddegh E, Torkzadeh-Mahani M. Synthesis and characterization of biocompatible chitosan/aminotetrazol nanoparticles as a novel nanocarrier for gene delivery. JMBS 2021; 12 (3) : 10
URL: http://biot.modares.ac.ir/article-22-18519-en.html
Synthesis and characterization of biocompatible chitosan/aminotetrazol nanoparticles as a novel nanocarrier for gene delivery
Elaheh Mosaddegh * 1, Masoud Torkzadeh-Mahani2
1- Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Graduate University of Advanced Technology,Kerman, 76315-117 P.O Box , emosaddegh@gmail.com
2- Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Graduate University of Advanced Technology,76315-117 P.O Box
Abstract:   (1648 Views)
This article has been attempting to present a novel and biocompatible nanocarrier based on chitosan biopolymer with high efficiency and low toxicity for gene delivery. At the first, 5-amino-1H-tetrazol reacted with (3-chloropropyl)trimethoxysilane and then, the obtained organosilane intermediate was used for functionalization of chitosan amino groups. The structure and chemical composition of the synthesized nanocarrier were fully characterized by FESEM, TEM, XRD, FTIR, Zeta potential and DLS analysis. At the fallowing, the MTT test was performed in order to investigation of the nanocarrier toxicity. Furthermore, Hek-293T cell line was used for investigation of the nanocarrier efficiency in gene transfection toward the cell. The loading capacity of plasmid was optimal at an N/P ratio of 3 with excellent protection of the plasmid. The results were shown that the novel nanocarrier is high efficient and has high potential to gene transfection and protection in addition of nontoxicity, biodegradability and cost effectively.
Article number: 10
Keywords: 5-amino-1H-tetrazol, chitosan, organosilane, nanocarrier, Hek-293T, gene delivery
Full-Text [PDF 978 kb]   (836 Downloads)    
Article Type: Original Research | Subject: Pharmaceutical Biotechnology
Received: 2018/04/5 | Accepted: 2021/03/16 | Published: 2022/07/2
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