Volume 10, Issue 4 (2019)                   JMBS 2019, 10(4): 519-525 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

khatami F, najafi F, yari F, khavari nejad R A. Comparison of the Efficiency of Iron-Magnetic Nanoparticles with CTAB and Rapid Detection Methods for DNA Extraction of Rose petal (Rosa hybrida L. cv. Vendetta). JMBS 2019; 10 (4) :519-525
URL: http://biot.modares.ac.ir/article-22-18813-en.html
1- Ph.D. Student of Plant Physiology, Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code: 1571914911, Tehran, Iran.
2- Associate Professor, Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code: 1571914911, Tehran, Iran. , najafi_f@khu.ac.ir
3- Assistant Professor, Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Postal Code: 33535111, Tehran, Iran.
4- Professor, Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code: 1571914911, Tehran, Iran; and Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Abstract:   (6166 Views)
The high quality and quantity of extracted DNA is necessary for a variety of molecular biology studies. Low yields and poor quality of genomic DNA extracted from petal due to high levels of secondary metabolites. Carotenoids, anthocyanins, phenolic acids and flavonoids are the most effective secondary metabolites in petals, which are considered as contaminating compounds and could lead to interfere with DNA during extraction and purification. Considering that the basis of the most molecular research in genetic engineering and genomics is high-quality of DNA, therefore, it seems that finding an efficient method for reducing adverse effects of these contaminating compounds for the exteraction is essential. In this regard, iron-magnetic nanoparticles have been used to improve the exteraction of high yields and quality of DNA from rose petals in the present work. In the following, to compare the efficiency of DNA extraction, modified CTAB (Cetyl Trimethyl Ammonium Bromide) and rapid detection methods were used. The results showed that petal’s extracted DNA quantification and qualification by iron-magnetic nanoparticles procedure was much more reliable than two other methods. Inaddition, this method could extract optimal amount of DNA with the lowest amounts of samples within few minutes. Due to high qualification and quantification of DNA purification by iron-magnetic nanoparticles, the present procedure could be recommended as an efficient protocol for rose petal DNA extraction.
Full-Text [PDF 1219 kb]   (2485 Downloads)    
Article Type: Original Research | Subject: Molecular biotechnology
Received: 2018/04/10 | Accepted: 2019/03/18 | Published: 2019/12/21

References
1. Narayanan C, Dubey S, Wali SA, Shukla N, Kumar R, Mandal AK, et al. Optimization of DNA extraction for ISSR studies in Tectona grandis L.f.-an important forest tree species. Afr J Biotechnol. 2006;5(13):1220-3. [Link]
2. Abdel-Latif A, Osman G. Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize. Plant Methods. 2017;13:1. [Link] [DOI:10.1186/s13007-016-0152-4]
3. Dehestani A, Kazemi Tabar SK. A rapid efficient method for DNA isolation from plants with high levels of secondary metabolites. Asian J Plant Sci. 2007;6(6):977-81. [Link] [DOI:10.3923/ajps.2007.977.981]
4. Sahu SK, Thangaraj M, Kathiresan K. DNA extraction protocol for plants with high levels of secondary metabolites and polysaccharides without using liquid nitrogen and phenol. ISRN Mol Biol. 2012;2012:205049. [Link] [DOI:10.5402/2012/205049]
5. Kumar V, Prasad A, Roy Ch, Chattopadhyay T. Validation of a simple and rapid method for isolating genomic DNA from medicinal and aromatic plants for subsequent polymerase chain reaction. Int J Curr Microbiol App Sci. 2018;7(8):2562-6. [Link] [DOI:10.20546/ijcmas.2018.708.263]
6. Varma A, Padh H, Shrivastava N. Plant genomic DNA isolation: An art or a science. Biotechnol J. 2007;2(3):386-92. [Link] [DOI:10.1002/biot.200600195]
7. Tan SC, Yiap BC. DNA, RNA, and protein extraction: The past and the present. J Biomed Biotechnol. 2009;2009:574398. [Link] [DOI:10.1155/2009/574398]
8. Khan IA, Awan FS, Ahmad A, Khan AA. A modified mini-prep method for economical and rapid extraction of genomic DNA in plants. Plant Mol Biol Rep. 2004;22(1):89. [Link] [DOI:10.1007/BF02773355]
9. Lutz KA, Wang W, Zdepski A, Michael TP. Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing. BMC Biotechnol. 2011;11:54. [Link] [DOI:10.1186/1472-6750-11-54]
10. Xu Q, Wen X, Deng X. A simple protocol for isolating genomic DNA from chestnut rose (Rosa roxburghii Tratt) for RFLP and PCR analyses. Plant Mol Biol Rep. 2004;22(3):301-2. [Link] [DOI:10.1007/BF02773140]
11. Cheng YJ, Guo WW, Yi HL, Pang XM, Deng X. An efficient protocol for genomic DNA extraction from Citrus species. Plant Mol Biol Rep. 2003;21(2):177-8. [Link] [DOI:10.1007/BF02774246]
12. Gabriadze I, Kutateladze T, Vishnepolsky B, Karseladze M, Datukishvili N. Application of PCR-based methods for rapid detection of corn ingredients in processed foods. Int J Nutr Food Sci. 2014;3(3):199-202. [Link]
13. Stefanova P, Taseva M, Georgieva T, Gotcheva V, Angelov A. A modified CTAB method for DNA extraction from soybean and meat products. Biotechnol Biotechnol Equip. 2013;27(3):3803-10. [Link] [DOI:10.5504/BBEQ.2013.0026]
14. Amani J, Kazemi R, Abbasi AR, Salmanian AH. A simple and rapid leaf genomic DNA extraction method for polymerase chain reaction analysis. Iran J Biotechnol. 2011;9(1):69-71. [Link]
15. Ghahari S, Ghahari S, Nematzadeh GA. Magnetic nano fluids for isolation of genomic DNA and total RNA from various prokaryote and eukaryote cells. J Chromatogr B. 2018;(1102-1103):125-34. [Link] [DOI:10.1016/j.jchromb.2018.10.006]
16. Cai BH, Zhang JY, Gao ZH, Qu SC, Tong ZG, Mi L, et al. An improved method for isolation of total DNA from the leaves of Fragaria spp. Jiangsu J Agric Sci. 2008;24(6);875-7. [Link]
17. Olgunsoy P, Ulusoy S, Akçay UÇ. Metabolite production and antibacterial activities of callus cultures from rosa damascena mill. petals. Marmara Pharm J. 2017;21(3):590-7. [Link] [DOI:10.12991/marupj.319331]
18. Yu D, Tang H, Zhang Y, Du Z, Yu H, Chen Q. Comparison and improvement of different methods of RNA isolation from strawberry (Fragria× ananassa). J Agric Sci. 2012;4(7):51-6. [Link] [DOI:10.5539/jas.v4n7p51]
19. Klie M, Debener T. Identification of superior reference genes for data normalisation of expression studies via quantitative PCR in hybrid roses (Rosa hybrida). BMC Res Notes. 2011;4(1):518. [Link] [DOI:10.1186/1756-0500-4-518]
20. Zhang ZC, Yuan C, Wan QH. Surface modification of magnetic silica microspheres and its application to the isolation of plant genomic nucleic acids. Chin J Anal Chem. 2007;35(1):31-6. [Link] [DOI:10.1016/S1872-2040(07)60024-3]
21. Kudr J, Haddad Y, Richtera L, Heger Z, Cernak M, Adam V, et al. Magnetic nanoparticles: From design and synthesis to real world applications. Nanomaterials. 2017;7(9):243. [Link] [DOI:10.3390/nano7090243]
22. Berensmeier S. Magnetic particles for the separation and purification of nucleic acids. Appl Microbial Biotechnol. 2006;73(3):495-504. [Link] [DOI:10.1007/s00253-006-0675-0]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.