Volume 10, Issue 3 (2019)
JMBS 2019, 10(3): 503-509 |
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Samimi H, Haghpanah V, Irani S, Fallah P, Arefian E, Soleimani M. Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture. JMBS 2019; 10 (3) :503-509
URL: http://biot.modares.ac.ir/article-22-23358-en.html
URL: http://biot.modares.ac.ir/article-22-23358-en.html
1- Biology Department, Basic Sciences Faculty, Science & Research Branch, Islamic Azad University, Tehran, Iran
2- Endocrinology & Metabolism Research Center, Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3- Laboratory Science Department, Allied Medicine Faculty, Alborz University of Medical Sciences, Karaj, Iran
4- Microbiology Department, Biology Faculty, College of Science, University of Tehran, Tehran, Iran
5- Hematology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. ,soleim_m@modares.ac.ir
2- Endocrinology & Metabolism Research Center, Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3- Laboratory Science Department, Allied Medicine Faculty, Alborz University of Medical Sciences, Karaj, Iran
4- Microbiology Department, Biology Faculty, College of Science, University of Tehran, Tehran, Iran
5- Hematology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. ,
Abstract: (4497 Views)
Aims: Three-dimensional (3D) cell culture systems are important because simulating the physiological microenvironment and representing more similarity to “in vivo” conditions for anticancer drug screening. Taking the advantages of 3D cell culture in the cancer therapy field, we have developed the 3D in vitro anaplastic thyroid cancer (ATC) model for determining the cytotoxic dose of "BI-847325" chemotherapy agent in ATC cell lines with different genetic background.
Materials and Methods: C643 and SW1736 ATC cell lines were grown in alginate scaffold. Beads were incubated in medium for one week. Cells were treated with different doses (1-64μM) of BI-847325 for 24h. The cytotoxic effect of BI-847325 on 3D cultured cell lines was studied by MTT [3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay. The survival rate of alginate-encapsulated cells was analyzed by CFSE (5, 6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining in effective doses for each of the cell lines.
Findings: Cytotoxic effect of BI-847325 anticancer drug was different for two ATC cell lines. Effective doses of BI-847325 for C643 and SW1736 cell lines were at 25μM and 43μM, respectively. CFSE staining analysis confirmed these data.
Conclusion: Overall, the results of the present study showed that the cytotoxic effect of BI-847325 chemotherapy agent was different for two ATC cell lines. The importance of this subject in regard to the 3D cell culture methods can be useful for researchers in the design of the complementary experience in order to achieve the most appropriate chemotherapy drug with the most effective dose.
Materials and Methods: C643 and SW1736 ATC cell lines were grown in alginate scaffold. Beads were incubated in medium for one week. Cells were treated with different doses (1-64μM) of BI-847325 for 24h. The cytotoxic effect of BI-847325 on 3D cultured cell lines was studied by MTT [3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay. The survival rate of alginate-encapsulated cells was analyzed by CFSE (5, 6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining in effective doses for each of the cell lines.
Findings: Cytotoxic effect of BI-847325 anticancer drug was different for two ATC cell lines. Effective doses of BI-847325 for C643 and SW1736 cell lines were at 25μM and 43μM, respectively. CFSE staining analysis confirmed these data.
Conclusion: Overall, the results of the present study showed that the cytotoxic effect of BI-847325 chemotherapy agent was different for two ATC cell lines. The importance of this subject in regard to the 3D cell culture methods can be useful for researchers in the design of the complementary experience in order to achieve the most appropriate chemotherapy drug with the most effective dose.
Article Type: Original Research |
Subject:
Pharmaceutical Biotechnology
Received: 2018/07/22 | Accepted: 2019/05/12 | Published: 2019/09/1
Received: 2018/07/22 | Accepted: 2019/05/12 | Published: 2019/09/1
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