Volume 9, Issue 3 (2018)                   JMBS 2018, 9(3): 325-330 | Back to browse issues page

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Ranaei Pirmardan E, Soheili Z, Samiei S, Ahmadieh H, Mowla S, Masoumi M et al . Acute Induction of Ganglion Cell Death and Generation of Mouse Model of Glaucoma by N-Methyl-D-Aspartate. JMBS 2018; 9 (3) :325-330
URL: http://biot.modares.ac.ir/article-22-24339-en.html
1- Molecular Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Molecular Medicine Department, Medical Biotechnology Institute, National Institute of Genetic Engineering & Biotechnology, Tehran, Iran, National Institute of Genetic Engineering & Biotechnology, Pajoohesh Boulevard, Pajoohesh Township, Kilometer 15, Tehran-Karaj Highway, Tehran, Iran. Postal Code: 1497716316 , soheili@nigeb.ac.ir
3- Blood Transfusion Research Center, High Institute for Research & Education in Transfusion Medicine, Tehran, Iran
4- Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- Molecular Medicine Department, Medical Biotechnology Institute, National Institute of Genetic Engineering & Biotechnology, Tehran, Iran
6- Molecular Medicine Department, Advanced Technologies in Medicine Faculty, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (8725 Views)
Aims: Glaucoma is an optic neuropathy that causes loss of retinal ganglion cells (RGC) and leads to blindness. This disease is a leading cause of blindness worldwide. For pre-clinical studies and finding novel therapies, using functional animal models is unavoidable. One of these models is the mice treated with N-Methyl-D-Aspartate (NMDA). The aim of this study was the acute induction of ganglion cell death and generation of mouse experimental model of glaucoma by N-Methyl-D-Aspartate.
Materials and Methods: In this experimental study, the creation of model mice with NMDA neurotoxin were created. For this purpose, retinal cell damage was induced in vivo in mice by intravitreal injection of NMDA. After removing the eyes, tissue analyses were performed on sample and control eyes. After tissue staining, the number of ganglion cells and the thickness of the retina layers and Ganglion Cell Complex (GCC) were evaluated. In addition, number of ganglion cells, thicknesses of the retina, and GCC of the optic nerve disc were measured in samples.
One-way ANOVA and SPSS 22 software were used to analyze the data.
Findings: Only 3 days after the injection to eye samples of NMDA, the thickness of the GCC and retinal layers as well as the number of ganglion cells significantly decreased compared to the control samples. The 50% reduction in the number of ganglion cells in the glucoma sample was confirmed.
Conclusion: Three days after the injection of NMDA to eye samples, the thickness of the GCC and retinal layers as well as the number of ganglion cells is significantly decreased compared to the control samples.
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Subject: Agricultural Biotechnology
Received: 2016/07/1 | Accepted: 2016/08/22 | Published: 2018/09/22

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