Volume 9, Issue 3 (2018)                   JMBS 2018, 9(3): 403-409 | Back to browse issues page

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Abbasi H, Kamrani E, Amrollahi Biuki N, Ehsanpoor M. Antibacterial, Antifungal, and Cytotoxic Effect of Stichodactyla haddoni from Persian Gulf . JMBS 2018; 9 (3) :403-409
URL: http://biot.modares.ac.ir/article-22-24443-en.html
1- Marine Biology Department, Marine Sciences & Technology Faculty, Hormozgan University, Bandar Abbas, Iran
2- Fisheries Department, Marine Sciences & Technology Faculty, Hormozgan University, Bandar Abbas, Iran, Hormozgan University, 9 Kilometer Minab Road, Bandar Abbas, Iran . Postal Code: 7916193145 , ezas47@gmail.com
3- Fisheries Department, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas, Iran
Abstract:   (3462 Views)
Aims: No study has been conducted on the antibacterial activity of sea anemone in the Persian Gulf region; thus, the aim of the present study was to investigate the antibacterial, antifungal, and cytotoxic effect of Stichodactyla haddoni from Persian Gulf.
Materials and Methods: In this experimental study, sea anemone samples were collected from the coast of Persian Gulf (Hormoz Island) and their extraction was performed with organic solvents such as methanol, dichloromethane, and acetone. The effect of the extract obtained on human pathogenic bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, and fungus strains such as Candida albicans, Aspergillus niger was tested, using agar disk diffusion method. The cytotoxicity of this anemic was evaluated, using brine shrimp test (BST) method on Artemia salina. The data were analyzed by SPSS 19, using Probit regression test.
Findings: The extracts studied in this study showed little anti-bacterial effect. In antifungal assay, inhibition zone was showing maximum of 17mm at 16µg concentration against Aspergillus niger of acetone extract of S.haddoni. The methanolic extracts of S.haddoni tissue had a higher cytotoxicity with less LC50 (609 330/μg/ml) than acetone and diclormethanic extracts.
Conclusion: Methanolic, acetone, and diclormethanic extracts, from S.haddoni have an antifungal effect on their antibacterial properties. It also has significant cytotoxic effects, which is higher in methanolic extract than acetone and diclormethanic extracts.
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Subject: Agricultural Biotechnology
Received: 2017/10/27 | Accepted: 2018/03/3 | Published: 2018/09/22

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