Volume 9, Issue 2 (2018)
JMBS 2018, 9(2): 267-275 |
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Noofeli M, Asadipour M, Yari R. Analysis of Housekeeping Genes in Bordetella pertussis Vaccine Strains by Multilocus Sequence Typing (MLST). JMBS 2018; 9 (2) :267-275
URL: http://biot.modares.ac.ir/article-22-25958-en.html
URL: http://biot.modares.ac.ir/article-22-25958-en.html
1- Human Bacterial Vaccines Production & Research Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education & Extension Organisation (AREEO), Tehran, Iran, Human Bacterial Vaccines Production & Research Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education & Extension Organisation (AREEO), Tehran, Iran. Postal Code: 3197619751 , Noofeli1234@yahoo.com
2- Cellular & Molecular Biology Department, Science Faculty, Boroujerd Branch, Islamic Azad University, Borujerd, Iran
3- Biology Department, Sciences Faculty, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2- Cellular & Molecular Biology Department, Science Faculty, Boroujerd Branch, Islamic Azad University, Borujerd, Iran
3- Biology Department, Sciences Faculty, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Abstract: (6738 Views)
Aims: Bordetella pertussis is a gram negative and obligated aerobic bacterium that causes pertussis disease and it is a specific pathogen in human. Pertussis is an acute respiratory infection and leads to death in infants. The aim of this study was to analyze housekeeping genes in Bordetella pertussis vaccine strains by multilocus sequence typing (MLST).
Materials and Methods: In the present experimental study, 4 samples of 134 and 509 bacterial strains and 2 standard samples of Tohama I, and 18323 were collected. After biochemical tests, the samples were cultured and separated and the genomic purification of DNA was done by Phenol–chloroform technique and analyzed by MLST. After genome sequencing, the analysis was performed by standard software such as Clustalw 2, MEGA 5.04, and DNASIS Max 3. Sequence similarity of 16S rDNA gene nucleotides was performed, using BLAST software with sequences recorded in the GenBank genome database to compare and determine the sequences similarity.
Findings: Regarding the created bands and the sequence of the game, the housekeeping genes in Bordetella pertussis vaccine strains were approved. The results of the PCR reaction for Pgm, Icd, Gly A, and Tyr B genes showed that all specimens have homogenous genes with a molecular weight of 500bp.
Conclusion: Evaluating the housekeeping genes in Bordetella pertussis vaccine strains by MLST vaccine strains (Razi Institute; Iran) correspond with international standard series and no change or deviation has occurred in the studied genes.
Materials and Methods: In the present experimental study, 4 samples of 134 and 509 bacterial strains and 2 standard samples of Tohama I, and 18323 were collected. After biochemical tests, the samples were cultured and separated and the genomic purification of DNA was done by Phenol–chloroform technique and analyzed by MLST. After genome sequencing, the analysis was performed by standard software such as Clustalw 2, MEGA 5.04, and DNASIS Max 3. Sequence similarity of 16S rDNA gene nucleotides was performed, using BLAST software with sequences recorded in the GenBank genome database to compare and determine the sequences similarity.
Findings: Regarding the created bands and the sequence of the game, the housekeeping genes in Bordetella pertussis vaccine strains were approved. The results of the PCR reaction for Pgm, Icd, Gly A, and Tyr B genes showed that all specimens have homogenous genes with a molecular weight of 500bp.
Conclusion: Evaluating the housekeeping genes in Bordetella pertussis vaccine strains by MLST vaccine strains (Razi Institute; Iran) correspond with international standard series and no change or deviation has occurred in the studied genes.
Subject:
Agricultural Biotechnology
Received: 2016/11/5 | Accepted: 2017/12/16 | Published: 2018/06/21
Received: 2016/11/5 | Accepted: 2017/12/16 | Published: 2018/06/21
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