Volume 11, Issue 1 (2020)
JMBS 2020, 11(1): 45-52 |
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ahmadi F, abbasi A, Yakhchali B, alizade H, Pourbabaee A. Extracellular peroxidase enzyme production in native bacteria Bacillus tequilensis and determination of some biochemical properties of enzyme. JMBS 2020; 11 (1) :45-52
URL: http://biot.modares.ac.ir/article-22-31367-en.html
URL: http://biot.modares.ac.ir/article-22-31367-en.html
1- Department of biotechnology, College of Agriculture & Natural Resources, University of Tehran
2- Department of agronomy and plant breeding, College of Agricultural Science & Engineering, University of Tehran ,rezabbasi@ut.ac.ir
3- National Institute of Genetic Engineering and Biotechnology
4- Department of agronomy and plant breeding, College of Agricultural Science & Engineering, University of Tehran
5- Department of Soil Sience Faculty of Agriculture Engineering and Technology College of Agriculture & Natural Resources, University of Tehran
2- Department of agronomy and plant breeding, College of Agricultural Science & Engineering, University of Tehran ,
3- National Institute of Genetic Engineering and Biotechnology
4- Department of agronomy and plant breeding, College of Agricultural Science & Engineering, University of Tehran
5- Department of Soil Sience Faculty of Agriculture Engineering and Technology College of Agriculture & Natural Resources, University of Tehran
Abstract: (4274 Views)
Aims: Peroxidases are used in a wide range of biotechnological processes, most of which are carried out at high temperatures and high pH levels. Since most of the commonly used peroxidases are unstable and inactive in alkaline conditions and high temprature, it is necessary to find thermoalkalophilic peroxidases for practical purposes.
Materials and Methods: In this study, extracellular production of peroxidase in the native strain Bacillus tequilensis was studied. for this purpose, Enzyme activity was evaluated using two substrates 2,4-DCP and pyrogallol in bacterial liquid culture and the effect of culture time on enzyme production, as well as the effect of parameters such as pH and temperature on enzyme activity investigated. The relative purification of the enzyme was performed using ion exchange chromatography with sephadex DEAE A50 and the kinetic parameters of enzyme activity were evaluated. In this study, kinetic parameters such as Km and Vmax were calculated.
Results: Measurement of enzyme activity at different times of culture indicated that the highest amount of peroxidase production was obtained 72 h after bacterial culture.
Materials and Methods: In this study, extracellular production of peroxidase in the native strain Bacillus tequilensis was studied. for this purpose, Enzyme activity was evaluated using two substrates 2,4-DCP and pyrogallol in bacterial liquid culture and the effect of culture time on enzyme production, as well as the effect of parameters such as pH and temperature on enzyme activity investigated. The relative purification of the enzyme was performed using ion exchange chromatography with sephadex DEAE A50 and the kinetic parameters of enzyme activity were evaluated. In this study, kinetic parameters such as Km and Vmax were calculated.
Results: Measurement of enzyme activity at different times of culture indicated that the highest amount of peroxidase production was obtained 72 h after bacterial culture.
Keywords: Bacillus tequilensis, peroxidase, thermoalkylphil, kinetic parameters, pyrogallol, 2, 4-DCP
Article Type: Original Research |
Subject:
Microbial biotechnology
Received: 2019/03/16 | Accepted: 2019/09/2 | Published: 2020/06/6
Received: 2019/03/16 | Accepted: 2019/09/2 | Published: 2020/06/6
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