1- student of urmia university, urmia
2- associated professor of urmia univeristy, urmia
Abstract: (13459 Views)
Abstract-Brain shrimp Artemia can tolerate different range of ecologic conditions. This animal is also known because of high nutrition value and special application in research. The species of Artemia franciscana found endemically on American continent, but recently it has been reported from Mahalru Lake, Iran. Due to differences between ecological conditions of Maharlu Lake with originally habitat of A. franciscana, Great Salt Lake, and taking to account the 10 years successful existence of this species in the lake, be believe that this species has adapted to new environment genetically. Given the fact that any kind of adaption can endanger endemic Maharlu parthenogenetic Artemia, in present study genetically differentiation between two populations of A. franciscana, before and after migration to new environment was studied. The study was conducted on structure of two important genes as COI and HSP26 using RCP-RFLP method. Successful digestion of the 700bp fragment of COI gene by two endonucleases enzymes, TaqI and EcoRI in addition to the digestion of 217bp fragment by Eco471 revealed genetic variation between two populations. The 7 generated specific haplotypes genetic distance of D=0.18 between the populations. According to the results and also successful residence of A. franciscana in Maharlu Lake, given the fact of high production performance of A. franciscana it is believed that the settler Artemia can be account as Iranian Artemia populations which will threaten existence of endemic parthenogenetic Artemia population in the lake.
Article Type:
Research Paper |
Subject:
Genetics|Soil Science Received: 2011/07/19 | Accepted: 2012/02/22 | Published: 2012/10/22