Volume 11, Issue 1 (2020)
JMBS 2020, 11(1): 13-20 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Soltani M, Ahmadian Chashmi N, Sharifi M, Fotovat R, Behmanesh M. Investigating the Cytotoxic Effect of Pinoresinol and Lariciresinol on Breast Cancer Cell Line SKBr3. JMBS 2020; 11 (1) :13-20
URL: http://biot.modares.ac.ir/article-22-34070-en.html
URL: http://biot.modares.ac.ir/article-22-34070-en.html
Investigating the Cytotoxic Effect of Pinoresinol and Lariciresinol on Breast Cancer Cell Line SKBr3
1- Plant Production & Genetics Department, Agriculture Faculty, University of Zanjan, Zanjan, Iran
2- Biology Department, Basic Sciences Faculty, University of Mazandaran, Babolsar, Iran
3- Plant Biology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
4- Plant Production & Genetics Department, Agriculture Faculty, University of Zanjan, Zanjan, Iran, Plant Production & Genetics Department, Agriculture Faculty, University of Zanjan, Zanjan, Iran. Postal Code: 4537138791 ,r_fotovat@znu.ac.ir
5- Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Biology Department, Basic Sciences Faculty, University of Mazandaran, Babolsar, Iran
3- Plant Biology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
4- Plant Production & Genetics Department, Agriculture Faculty, University of Zanjan, Zanjan, Iran, Plant Production & Genetics Department, Agriculture Faculty, University of Zanjan, Zanjan, Iran. Postal Code: 4537138791 ,
5- Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
Abstract: (5144 Views)
Aims: Breast cancer is the most common malignancy in women throughout the world. Among the various methods for preventing and treating cancer, plant natural compounds have more benefits than chemical drugs and have less side effects. Recently, many studies have been carried out on the antioxidant, anticancer, antiproliferation, and antiinflammatory properties of plant lignans, indicating the importance of these compounds in the prevention and treatment of various diseases. In the present study, the cytotoxic effects and inducing apoptosis of pinoresinol and lariciresinol lignans were investigated on SKBr3 breast cancer cell line.
Materials & Methods: SKBr3 cells were treated with different concentrations of both pinoresinol and lariciresinol, separately for 72 hours. Then, cell viability and cells morphological changes were determined using MTT assay and inverted light microscope, respectively. Also, apoptosis induction was analyzed by flow cytometry using an Annexin V-FITC apoptosis detection kit.
Findings: Both pinoresinol and lariciresinol treatments induced morphological changes, decreased cell growth, survival, proliferation and a significant increase in apoptosis induction in SKBr3 cell line compared to control cells in a concentration-dependent manner.
Conclusion: Inducing apoptosis and preventing the growth and proliferation of cancer cells are important mechanisms in the treatment of cancer. Pinoresinol and lariciresinol can be used to reduce cell proliferation and increase apoptosis induction in prevention and treatment of breast cancer.
Materials & Methods: SKBr3 cells were treated with different concentrations of both pinoresinol and lariciresinol, separately for 72 hours. Then, cell viability and cells morphological changes were determined using MTT assay and inverted light microscope, respectively. Also, apoptosis induction was analyzed by flow cytometry using an Annexin V-FITC apoptosis detection kit.
Findings: Both pinoresinol and lariciresinol treatments induced morphological changes, decreased cell growth, survival, proliferation and a significant increase in apoptosis induction in SKBr3 cell line compared to control cells in a concentration-dependent manner.
Conclusion: Inducing apoptosis and preventing the growth and proliferation of cancer cells are important mechanisms in the treatment of cancer. Pinoresinol and lariciresinol can be used to reduce cell proliferation and increase apoptosis induction in prevention and treatment of breast cancer.
Article Type: Original Research |
Subject:
Agricultural Biotechnology
Received: 2019/06/24 | Accepted: 2019/09/18 | Published: 2020/03/18
Received: 2019/06/24 | Accepted: 2019/09/18 | Published: 2020/03/18
References
1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394-424. [Link] [DOI:10.3322/caac.21492]
2. Venkatadri R, Muni T, Iyer AK, Yakisich JS, Azad N. Role of apoptosis-related miRNAs in resveratrol-induced breast cancer cell death. Cell Death Dis. 2016;7(2):e2104. [Link] [DOI:10.1038/cddis.2016.6]
3. Rezapour Kalkhoran M, Kazerouni F, Omrani MD, Rahimipour A, Shanaki M, Dehghan Nayeri N, et al. Cytotoxic effect of emodin on growth of SKBR3 breast cancer cells. Int J Cancer Manag. 2017;10(4):e8094. [Link] [DOI:10.5812/ijcm.8094]
4. Ahmadi A, Ramazani R, Rezagholi T, Yavari P. Incidence pattern and spatial analysis of breast cancer in Iranian women: Geographical information system applications. East Mediterr Health J. 2018;24(4):360-7. [Link] [DOI:10.26719/2018.24.4.360]
5. Nafissi N, Khayamzadeh M, Zeinali Z, Pazooki D, Hosseini M, Akbari ME. Epidemiology and histopathology of breast cancer in Iran versus other Middle Eastern countries. Middle East J Cancer. 2018;9(3):243-51. [Link]
6. Gali-Muhtasib H, Hmadi R, Kareh M, Tohme R, Darwiche N. Cell death mechanisms of plant-derived anticancer drugs: beyond apoptosis. Apoptosis. 2015;20(12):1531-62. [Link] [DOI:10.1007/s10495-015-1169-2]
7. Ardalani H, Avan A, Ghayour Mobarhan M. Podophyllotoxin: A novel potential natural anticancer agent. Avicenna J Phytomed. 2017;7(4):285-94. [Link]
8. Fantini M, Benvenuto M, Masuelli L, Frajese GV, Tresoldi I, Modesti A, et al. In vitro and in vivo antitumoral effects of combinations of polyphenols, or polyphenols and anticancer drugs: Perspectives on cancer treatment. Int J Mol Sci. 2015;16(5):9236-82. [Link] [DOI:10.3390/ijms16059236]
9. Tsopmo A, Awah FM, Kuete V. Lignans and stilbenes from African medicinal plants. In: Kuete V, editor. Medicinal plant research in Africa: Pharmacology and chemistry. 1st Edition. Amsterdam: Elsevier;2013. pp. 435-78. [Link] [DOI:10.1016/B978-0-12-405927-6.00012-6]
10. Cheshomi H, Aldaghi LS, Rezaei Seresht H. Cytotoxicity of the methanol extract of datura innoxia petals on MCF-7 and HEK-293 cell lines. J Biomed. 2016;1(2):e6623. [Link] [DOI:10.17795/jmb-6623]
11. Sadeghi I, Behmanesh M, Ahmadian Chashmi N, Sharifi M, Soltani BM. 6-Methoxy podophyllotoxin induces apoptosis via inhibition of TUBB3 and TOPIIA gene expressions in 5637 and K562 cancer cell lines. Cell J. 2015;17(3):502-9. [Link]
12. Thompson LU, Seidl MM, Rickard SE, Orcheson LJ, Fong HH. Antitumorigenic effect of a mammalian lignan precursor from flaxseed. Nutr Cancer. 1996;26(2):159-65. [Link] [DOI:10.1080/01635589609514472]
13. During A, Debouche C, Raas T, Larondelle Y. Among plant lignans, pinoresinol has the strongest antiinflammatory properties in human intestinal Caco-2 cells. J Nutr. 2012;142(10):1798-805. [Link] [DOI:10.3945/jn.112.162453]
14. Bajpai VK, Alam MB, Quan KT, Kwon KR, Ju MK, Choi HJ, et al. Antioxidant efficacy and the upregulation of Nrf2-mediated HO-1 expression by (+)-lariciresinol, a lignan isolated from Rubia philippinensis, through the activation of p38. Sci Rep. 2017;7:46035. [Link] [DOI:10.1038/srep46035]
15. Ma ZJ, Lu L, Yang JJ, Wang XX, Su G, Wang ZL, et al. Lariciresinol induces apoptosis in HepG2 cells via mitochondrial-mediated apoptosis pathway. Eur J Pharmacol. 2018;821:1-0. [Link] [DOI:10.1016/j.ejphar.2017.12.027]
16. Lee JH, Khor TO, Shu L, Su ZY, Fuentes F, Kong AN. Dietary phytochemicals and cancer prevention: Nrf2 signaling, epigenetics, and cell death mechanisms in blocking cancer initiation and progression. Pharmacol Ther. 2013;137(2):153-71. [Link] [DOI:10.1016/j.pharmthera.2012.09.008]
17. Safarzadeh E, Sandoghchian Shotorbani S, Baradaran B. Herbal medicine as inducers of apoptosis in cancer treatment. Adv Pharm Bull. 2014;4(Suppl 1):421-7. [Link]
18. Ji CF, Ji YB. Apoptosis of human gastric cancer SGC-7901 cells induced by podophyllotoxin. Exp Ther Med. 2014;7(5):1317-22. [Link] [DOI:10.3892/etm.2014.1606]
19. Kelly MG, Hartwell JL. The biological effects and the chemical composition of podophyllin. A review. J Natl Cancer Inst. 1954;14(4):967-1010. [Link]
20. Zi CT, Yang L, Xu FQ, Dong FW, Yang D, Li Y, et al. Synthesis and anticancer activity of dimeric podophyllotoxin derivatives. Drug Des Dev Ther. 2018;12:3393-406. [Link] [DOI:10.2147/DDDT.S167382]
21. Kamińska M, Ciszewski T, Łopacka-Szatan K, Miotła P, Starosławska E. Breast cancer risk factors. Prz Menopauzalny. 2015;14(3):196-202. [Link] [DOI:10.5114/pm.2015.54346]
22. Satake H, Ono E, Murata J. Recent advances in the metabolic engineering of lignan biosynthesis pathways for the production of transgenic plant-based foods and supplements. J Agricu Food Chem. 2013;61(48):11721-9. [Link] [DOI:10.1021/jf4007104]
23. Peng XH, Karna P, O'Regan RM, Liu X, Naithani R, Moriarty RM, et al. Down-regulation of inhibitor of apoptosis proteins by deguelin selectively induces apoptosis in breast cancer cells. Mol Pharmacol. 2007;71(1):101-11. [Link] [DOI:10.1124/mol.106.027367]
24. Kuo HJ, Wei ZY, Lu PC, Huang PL, Lee KT. Bioconversion of pinoresinol into matairesinol by use of recombinant Escherichia coli. Appl Environ Microbiol. 2014;80(9):2687-92. [Link] [DOI:10.1128/AEM.03397-13]
25. Satake H, Koyama T, Esmaeilzadeh Bahabadi S, Matsumoto E, Ono E, Murata J. Essences in metabolic engineering of lignan biosynthesis. Metabolites. 2015;5(2):270-90. [Link] [DOI:10.3390/metabo5020270]
26. Airio M, Wärri A, Mäkelä S, Saarinen NM. In vitro proliferation assays do not fully predict the MCF-7 tumor growth response to lignan lariciresinol in vivo. Proc Am Assoc Cancer Res. 2005;46:1223-1224. [Link]
27. López-Biedma A, Sánchez-Quesada C, Beltrán G, Delgado-Rodríguez M, Gaforio JJ. Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses. BMC Complement Altern Med. 2016;16(1):350. [Link] [DOI:10.1186/s12906-016-1233-7]
28. Sepporta MV, Mazza T, Morozzi G, Fabiani R. Pinoresinol inhibits proliferation and induces differentiation on human HL60 leukemia cells. Nutr Cancer. 2013;65(8):1208-18. [Link] [DOI:10.1080/01635581.2013.828089]
29. Pfeffer CM, Singh AT. Apoptosis: A target for anticancer therapy. Int J Mol Sci. 2018;19(2):448. [Link] [DOI:10.3390/ijms19020448]
30. Bagheri E, Hajiaghaalipour F, Nyamathulla Sh, Salehen NA. The apoptotic effects of Brucea javanica fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation. Drug Des Dev Ther. 2018;12:657-71. [Link] [DOI:10.2147/DDDT.S155115]
31. Porameesanaporn Y, Uthaisang-Tanechpongtamb W, Jarintanan F, Jongrungruangchok S, Thanomsub Wongsatayanon B. Terrein induces apoptosis in HeLa human cervical carcinoma cells through p53 and ERK regulation. Oncol Rep. 2013;29(4):1600-8. [Link] [DOI:10.3892/or.2013.2288]
32. Ouyang L, Shi Z, Zhao S, Wang FT, Zhou TT, Liu B, et al. Programmed cell death pathways in cancer: A review of apoptosis, autophagy and programmed necrosis. Cell Prolif. 2012;45(6):487-98. [Link] [DOI:10.1111/j.1365-2184.2012.00845.x]
33. Ağalar HG, Çiftçi GA, Göger F, Kırımer N. Activity guided fractionation of Arum italicum miller tubers and the LC/MS-MS profiles. Rec Nat Prod. 2018;12(1):64-75. [Link] [DOI:10.25135/rnp.06.17.05.089]
34. Fini L, Hotchkiss E, Fogliano V, Graziani G, Romano M, De Vol EB, et al. Chemopreventive properties of pinoresinol-rich olive oil involve a selective activation of the ATM-p53 cascade in colon cancer cell lines. Carcinogenesis. 2008;29(1):139-46. [Link] [DOI:10.1093/carcin/bgm255]
35. Zhang L, Si J, Li G, Li X, Zhang L, Gao L, et al. Umbelliprenin and lariciresinol isolated from a long-term-used herb medicine Ferula sinkiangensis induce apoptosis and G0/G1 arresting in gastric cancer cells. RSC Adv. 2015;5(110):91006-17. [Link] [DOI:10.1039/C5RA11335K]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |